Agarose gel electrophoresis of cerebrospinal fluid proteins of dogs after sample concentration using a membrane microconcentrator technique

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dc.contributor.authorVelasque Gama, Fernanda Gomes
dc.contributor.authorSantana, Aureo Evangelista
dc.contributor.authorde Campos Filho, Eugenio
dc.contributor.authorda Silva Nogueira, Claudia Aparecida
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:19:25Z
dc.date.available2014-05-20T15:19:25Z
dc.date.issued2007-03-01
dc.description.abstractBackground: Cerebrospinal fluid (CSF) is produced in the cerebral ventricles through ultrafiltration of plasma and active transport mechanisms. Evaluation of proteins in CSF may provide important information about the production of immunoglobulins within the central nervous system as well as possible disturbances in the blood-brain barrier. Objective: the objective of this study was to measure the concentration and fractions of protein in CSF samples using a membrane microconcentrator technique followed by electrophoresis, and to compare the protein fractions obtained with those in serum. Methods: CSF samples from 3 healthy dogs and 3 dogs with canine distemper virus infection were concentrated using a membrane microconcentrator having a 0.5 to 30,000 d nominal molecular weight limit (Ultrafree, Millipore, Billerica, MA, USA). Protein concentration was determined before and after concentration. Agarose gel electrophoresis was done on concentrated CSF samples, serum, and serial dilutions of one of the CSF samples. Results: Electrophoretic bands were clearly identified in densitometer tracings in CSF samples with protein concentrations as low as 1.3 g/dL. The higher CSF protein concentration in dogs with distemper was mainly the result of increased albumin concentration. Conclusion: the microconcentrating method used in this study enables characterization of the main protein fractions in CSF by routine electrophoresis and may be useful for interpreting the underlying cause of changes in CSF protein concentrationsen
dc.description.affiliationUniv Estadual Paulista, Fac Ciências Agr & Vet, Dept Vet Clin & Surg, São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciências Agr & Vet, Dept Vet Clin & Surg, São Paulo, Brazil
dc.format.extent85-88
dc.identifierhttp://dx.doi.org/10.1111/j.1939-165X.2007.tb00187.x
dc.identifier.citationVeterinary Clinical Pathology. Baton Rouge: Amer Soc Veterinary Clinical Pathology, v. 36, n. 1, p. 85-88, 2007.
dc.identifier.doi10.1111/j.1939-165X.2007.tb00187.x
dc.identifier.issn0275-6382
dc.identifier.urihttp://hdl.handle.net/11449/30902
dc.identifier.wosWOS:000248955200015
dc.language.isoeng
dc.publisherAmer Soc Veterinary Clinical Pathology
dc.relation.ispartofVeterinary Clinical Pathology
dc.relation.ispartofjcr0.937
dc.relation.ispartofsjr0,647
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectagarose gelpt
dc.subjectcerebrospinal fluidpt
dc.subjectdogpt
dc.subjectelectrophoresispt
dc.subjectglobulinspt
dc.subjectproteinpt
dc.titleAgarose gel electrophoresis of cerebrospinal fluid proteins of dogs after sample concentration using a membrane microconcentrator techniqueen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderAmer Soc Veterinary Clinical Pathology
unesp.author.lattes8905659987198068[2]
unesp.author.orcid0000-0003-4539-1802[2]

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