Isolate((R)) and Optiprep((R)) minigradients as alternatives for sperm selection in bovine in vitro embryo production

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dc.contributor.authorVianna, L. L.
dc.contributor.authorPradiee, J.
dc.contributor.authorSantos, E. C. S.
dc.contributor.authorGoncalves, A. O.
dc.contributor.authorPfeifer, L. F. M.
dc.contributor.authorRheingantz, M. G. T.
dc.contributor.authorDode, M. A. N.
dc.contributor.authorVieira, A. D.
dc.contributor.authorLima, V. F. H. [UNESP]
dc.contributor.authorCorrea, M. N.
dc.contributor.authorPegoraro, L. M. C.
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-12-03T13:08:47Z
dc.date.available2014-12-03T13:08:47Z
dc.date.issued2014-03-01
dc.description.abstractThe objective of this study was to evaluate alternatives in small volumes to conventional gradient of Percoll((R)) on semen quality, in vitro embryo production, sex ratio and embryo survival after vitrification. Thawed semen was randomly allocated to one of four density gradient selection methods: (1) conventional Percoll((R)) (P), (2) MiniPercoll (MP), (3) MiniIsolate (MI), and (4) MiniOptiprep (MO). Sperm kinetics and quality were evaluated. Use of P, MP and MI gradients did not affect sperm motility (P > 0.05). However, there was a decrease in total and progressive sperm motility in MO (70.8 and 51.3% vs. 87.3 and 69.5% for P; 87.3 and 73% for MP; 92.3 and 78.8% for MI; P < 0.05). The MO had lower membrane integrity compared with P, MP and MI (39.7 vs. 70.5, 72.3, 63.8%, respectively, P < 0.05). The percentage of blastocysts produced was higher in MI than in MP and MO (21.1 vs. 16.1 and 16.9%, P < 0.05) and similar to P (18.4%; P > 0.05). Sex ratio and embryo survival after vitrification were similar among groups (P > 0.05). Semen selected by Isolate and Optiprep gradient, at the concentrations and small volumes used, demonstrated similar characteristics and in vitro embryo production to conventional Percoll((R)) gradient.en
dc.description.affiliationUniv Fed Pelotas UFPel, Pelotas, RS, Brazil
dc.description.affiliationEmbrapa Clima Temperado, Pelotas, RS, Brazil
dc.description.affiliationEmbrapa Rondonia, Porto Velho, RO, Brazil
dc.description.affiliationEmbrapa Recursos Genet & Biotecnol, Brasilia, DF, Brazil
dc.description.affiliationUniv Estado Sao Paulo, UNESP, Jaboticabal, SP, Brazil
dc.description.affiliationUnespUniv Estado Sao Paulo, UNESP, Jaboticabal, SP, Brazil
dc.format.extent35-42
dc.identifierhttp://dx.doi.org/10.4141/CJAS2013-101
dc.identifier.citationCanadian Journal Of Animal Science. Ottawa: Agricultural Inst Canada, v. 94, n. 1, p. 35-42, 2014.
dc.identifier.doi10.4141/CJAS2013-101
dc.identifier.fileWOS000334249800005.pdf
dc.identifier.issn0008-3984
dc.identifier.urihttp://hdl.handle.net/11449/111588
dc.identifier.wosWOS:000334249800005
dc.language.isoeng
dc.publisherAgricultural Inst Canada
dc.relation.ispartofCanadian Journal of Animal Science
dc.relation.ispartofjcr0.657
dc.relation.ispartofsjr0,403
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectIn vitro fertilizationen
dc.subjectsperm selectionen
dc.subjectsex ratioen
dc.subjectsperm qualityen
dc.subjectcryopreservationen
dc.titleIsolate((R)) and Optiprep((R)) minigradients as alternatives for sperm selection in bovine in vitro embryo productionen
dc.typeArtigo
dcterms.rightsHolderAgricultural Inst Canada
unesp.author.orcid0000-0002-9756-7251[2]

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