Parasite Killing of Leishmania (V) braziliensis by Standardized Propolis Extracts

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dc.contributor.authorReboucas-Silva, Jessica
dc.contributor.authorCeles, Fabiana S.
dc.contributor.authorLima, Jonilson Berlink
dc.contributor.authorBarud, Hernane S. [UNESP]
dc.contributor.authorOliveira, Camila I. de
dc.contributor.authorBerretta, Andresa A.
dc.contributor.authorBorges, Valeria M.
dc.contributor.institutionFundacao Oswaldo Cruz
dc.contributor.institutionUniversidade Federal da Bahia (UFBA)
dc.contributor.institutionUniv Fed Oeste Bahia
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Araraquara
dc.contributor.institutionApis Flora Ind & Comercial Ltda
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2018-11-26T15:44:20Z
dc.date.available2018-11-26T15:44:20Z
dc.date.issued2017-01-01
dc.description.abstractTreatments based on antimonials to cutaneous leishmaniasis (CL) entail a range of toxic side effects. Propolis, a natural compound widely used in traditional medical applications, exhibits a range of biological effects, including activity against infectious agents. The aim of this study was to test the potential leishmanicidal effects of different propolis extracts against Leishmania (Viannia) braziliensis promastigotes and intracellular amastigotes in vitro. Stationary-phase L. (V) braziliensis promastigotes were incubated with mediumalone or treated with dry, alcoholic, or glycolic propolis extract (10, 50, or 100 mu g/mL) for 96 h. Our data showed that all extracts exhibited a dose-dependent effect on the viability of L. (V) braziliensis promastigotes, while controlling the parasite burden inside infected macrophages. Dry propolis extract significantly modified the inflammatory profile of murine macrophages by downmodulating TGF-beta and IL-10 production, while upmodulating TNF-alpha All three types of propolis extractwere found to reduce nitric oxide and superoxide levels in activated L. braziliensis-infected macrophages. Altogether, our results showed that propolis extracts exhibited a leishmanicidal effect against both stages of L. (V) braziliensis. The low cell toxicity and efficient microbicidal effect of alcoholic or glycolic propolis extracts make them candidates to an additive treatment for cutaneous leishmaniasis.en
dc.description.affiliationFundacao Oswaldo Cruz, Inst Goncalo Moniz, Salvador, BA, Brazil
dc.description.affiliationUniv Fed Bahia, Salvador, BA, Brazil
dc.description.affiliationUniv Fed Oeste Bahia, Ctr Ciencias Biol & Saude, Barreiras, BA, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Quim, Araraquara, SP, Brazil
dc.description.affiliationUniv Araraquara, Programa Posgrad Biotecnol, Araraquara, SP, Brazil
dc.description.affiliationApis Flora Ind & Comercial Ltda, Lab Pesquisa Desenvolvimento & Inovacao, Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, Ave Cafe S-N, BR-14049900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Quim, Araraquara, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFinanciadora de Estudos e Projetos, FINEP
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdCNPq: 309988/2014-4
dc.description.sponsorshipIdFAPESP: 2013/50496-2
dc.description.sponsorshipIdFinanciadora de Estudos e Projetos, FINEP: 03.12.0056.00
dc.format.extent14
dc.identifierhttp://dx.doi.org/10.1155/2017/6067172
dc.identifier.citationEvidence-based Complementary And Alternative Medicine. London: Hindawi Ltd, 14 p., 2017.
dc.identifier.doi10.1155/2017/6067172
dc.identifier.fileWOS000403240600001.pdf
dc.identifier.issn1741-427X
dc.identifier.urihttp://hdl.handle.net/11449/159573
dc.identifier.wosWOS:000403240600001
dc.language.isoeng
dc.publisherHindawi Ltd
dc.relation.ispartofEvidence-based Complementary And Alternative Medicine
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleParasite Killing of Leishmania (V) braziliensis by Standardized Propolis Extractsen
dc.typeArtigo
dcterms.rightsHolderHindawi Ltd
unesp.author.orcid0000-0002-1403-4768[3]

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