Parasite Killing of Leishmania (V) braziliensis by Standardized Propolis Extracts
dc.contributor.author | Reboucas-Silva, Jessica | |
dc.contributor.author | Celes, Fabiana S. | |
dc.contributor.author | Lima, Jonilson Berlink | |
dc.contributor.author | Barud, Hernane S. [UNESP] | |
dc.contributor.author | Oliveira, Camila I. de | |
dc.contributor.author | Berretta, Andresa A. | |
dc.contributor.author | Borges, Valeria M. | |
dc.contributor.institution | Fundacao Oswaldo Cruz | |
dc.contributor.institution | Universidade Federal da Bahia (UFBA) | |
dc.contributor.institution | Univ Fed Oeste Bahia | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.contributor.institution | Univ Araraquara | |
dc.contributor.institution | Apis Flora Ind & Comercial Ltda | |
dc.contributor.institution | Universidade de São Paulo (USP) | |
dc.date.accessioned | 2018-11-26T15:44:20Z | |
dc.date.available | 2018-11-26T15:44:20Z | |
dc.date.issued | 2017-01-01 | |
dc.description.abstract | Treatments based on antimonials to cutaneous leishmaniasis (CL) entail a range of toxic side effects. Propolis, a natural compound widely used in traditional medical applications, exhibits a range of biological effects, including activity against infectious agents. The aim of this study was to test the potential leishmanicidal effects of different propolis extracts against Leishmania (Viannia) braziliensis promastigotes and intracellular amastigotes in vitro. Stationary-phase L. (V) braziliensis promastigotes were incubated with mediumalone or treated with dry, alcoholic, or glycolic propolis extract (10, 50, or 100 mu g/mL) for 96 h. Our data showed that all extracts exhibited a dose-dependent effect on the viability of L. (V) braziliensis promastigotes, while controlling the parasite burden inside infected macrophages. Dry propolis extract significantly modified the inflammatory profile of murine macrophages by downmodulating TGF-beta and IL-10 production, while upmodulating TNF-alpha All three types of propolis extractwere found to reduce nitric oxide and superoxide levels in activated L. braziliensis-infected macrophages. Altogether, our results showed that propolis extracts exhibited a leishmanicidal effect against both stages of L. (V) braziliensis. The low cell toxicity and efficient microbicidal effect of alcoholic or glycolic propolis extracts make them candidates to an additive treatment for cutaneous leishmaniasis. | en |
dc.description.affiliation | Fundacao Oswaldo Cruz, Inst Goncalo Moniz, Salvador, BA, Brazil | |
dc.description.affiliation | Univ Fed Bahia, Salvador, BA, Brazil | |
dc.description.affiliation | Univ Fed Oeste Bahia, Ctr Ciencias Biol & Saude, Barreiras, BA, Brazil | |
dc.description.affiliation | Univ Estadual Paulista, Inst Quim, Araraquara, SP, Brazil | |
dc.description.affiliation | Univ Araraquara, Programa Posgrad Biotecnol, Araraquara, SP, Brazil | |
dc.description.affiliation | Apis Flora Ind & Comercial Ltda, Lab Pesquisa Desenvolvimento & Inovacao, Ribeirao Preto, SP, Brazil | |
dc.description.affiliation | Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, Ave Cafe S-N, BR-14049900 Ribeirao Preto, SP, Brazil | |
dc.description.affiliationUnesp | Univ Estadual Paulista, Inst Quim, Araraquara, SP, Brazil | |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.description.sponsorship | Financiadora de Estudos e Projetos, FINEP | |
dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
dc.description.sponsorshipId | CNPq: 309988/2014-4 | |
dc.description.sponsorshipId | FAPESP: 2013/50496-2 | |
dc.description.sponsorshipId | Financiadora de Estudos e Projetos, FINEP: 03.12.0056.00 | |
dc.format.extent | 14 | |
dc.identifier | http://dx.doi.org/10.1155/2017/6067172 | |
dc.identifier.citation | Evidence-based Complementary And Alternative Medicine. London: Hindawi Ltd, 14 p., 2017. | |
dc.identifier.doi | 10.1155/2017/6067172 | |
dc.identifier.file | WOS000403240600001.pdf | |
dc.identifier.issn | 1741-427X | |
dc.identifier.uri | http://hdl.handle.net/11449/159573 | |
dc.identifier.wos | WOS:000403240600001 | |
dc.language.iso | eng | |
dc.publisher | Hindawi Ltd | |
dc.relation.ispartof | Evidence-based Complementary And Alternative Medicine | |
dc.rights.accessRights | Acesso aberto | |
dc.source | Web of Science | |
dc.title | Parasite Killing of Leishmania (V) braziliensis by Standardized Propolis Extracts | en |
dc.type | Artigo | |
dcterms.rightsHolder | Hindawi Ltd | |
unesp.author.orcid | 0000-0002-1403-4768[3] |
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