Platelets can be a biological compartment for the Hepatitis C Virus

dc.contributor.authorAriede, Jovita Ramos [UNESP]
dc.contributor.authorPardini, Maria Inês De Moura Campos [UNESP]
dc.contributor.authorSilva, Giovanni Faria [UNESP]
dc.contributor.authorGrotto, Rejane Maria Tommasini [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-08-26T19:19:03Z
dc.date.available2015-08-26T19:19:03Z
dc.date.issued2015-06-01
dc.description.abstractAlthough HCV has hepatic tropism, the presence of the virus in extra-hepatic compartments has been well documented. Platelets have been described as carriers of the virus in the circulation and may be a natural reservoir for the virus. However, few studies have been performed to evaluate the levels of HCV RNA in plasma and platelets are equal or differ in some way. Therefore, the aim of this study was to perform a comparative evaluation of the stability of HCV RNA in plasma and isolated platelets. Four aliquots of whole plasma obtained from patients infected with HCV were incubated at 37 °C for 0, 48, 96 and 144 h. After incubation, the plasma and platelet pellet was obtained from each aliquot. Viral RNA in plasma and platelets was quantified by q-PCR. The results showed a decrease in HCV RNA levels in plasma with incubation time. However, platelet HCV RNA levels were stable up to 144 h incubation. The results of this study showed that HCV RNA in platelets, although at lower concentrations than in plasma, is preserved from degradation over time, suggesting that the virus may persist longer in the body when associated with platelets, which could have an impact on the efficiency of antiviral therapy.en
dc.description.affiliationUniversidade Estadual Paulista Faculdade de Medicina de Botucatu Divisão Hemocentro
dc.description.affiliationUniversidade Estadual Paulista Faculdade de Medicina de Botucatu Departamento de Clínica Médica
dc.description.affiliationUniversidade Estadual Paulista Faculdade de Ciências Agronômicas Fazenda Experimental de Lageado
dc.description.affiliationUnespUniversidade Estadual Paulista Faculdade de Medicina de Botucatu Divisão Hemocentro
dc.description.affiliationUnespUniversidade Estadual Paulista Faculdade de Medicina de Botucatu Departamento de Clínica Médica
dc.description.affiliationUnespUniversidade Estadual Paulista Faculdade de Ciências Agronômicas Fazenda Experimental de Lageado
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent627-629
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200627&lng=en&nrm=iso&tlng=en
dc.identifier.citationBrazilian Journal of Microbiology, v. 46, n. 2, p. 627-629, 2015.
dc.identifier.doi10.1590/S1517-838246220140553
dc.identifier.fileS1517-83822015000200627.pdf
dc.identifier.issn1517-8382
dc.identifier.lattes6322604200510676
dc.identifier.lattes4619588334582084
dc.identifier.lattes7788448564326585
dc.identifier.orcid0000-0002-4035-9486
dc.identifier.scieloS1517-83822015000200627
dc.identifier.urihttp://hdl.handle.net/11449/127296
dc.language.isoeng
dc.publisherSociedade Brasileira de Microbiologia
dc.relation.ispartofBrazilian Journal of Microbiology
dc.relation.ispartofjcr1.810
dc.relation.ispartofsjr0,630
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectHepatitis C Virusen
dc.subjectplateletsen
dc.subjectViral loaden
dc.subjectVirological relapseen
dc.titlePlatelets can be a biological compartment for the Hepatitis C Virusen
dc.typeArtigo
unesp.author.lattes6322604200510676
unesp.author.lattes4619588334582084
unesp.author.lattes7788448564326585[4]
unesp.author.orcid0000-0002-4035-9486[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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