Relationship between carbon source, production and pattern action of alpha-amilase from Rhizopus sp

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dc.contributor.authorCruz, R.
dc.contributor.authordeSouza, E. L.
dc.contributor.authorHoffmann, EHE
dc.contributor.authorBellini, M. Z.
dc.contributor.authorCruz, VDA
dc.contributor.authorVieira, C. R.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:28:04Z
dc.date.available2014-05-20T15:28:04Z
dc.date.issued1997-04-01
dc.description.abstractThis paper discusses the inducer effect of corn soluble starch and the individual components (amylose and amylopectin) from corn and potatoes starch for alpha-amylase production by a strain of Rhizopus sp. The following decreasing order in the enzyme production was obtained: corn amylose > potatoes amylose > corn amylopectin > potatoes amylopectin > starch > maltose, coinciding with the ability of the enzyme to release reducing units, except the soluble starch that was more softly hydrolysed. However, when the enzyme action was measured by the iodine binding method, an inverse order of enzyme activity was obtained, that is: amylopectins > starch > amylosis. The results suggest that: a) branched structures in substrate affect the enzyme production; b) corn amylose and corn amylopectin are better inducers than their respectives homologous from potatoes; c) cc-amylase from Rhizopus sp has different action patterns on substrates with straight or branched chains: from the former, it removes only reducing units with lower molecular weight (G1-G3); from the latter it also removes oligosaccharides with higher molecular weight (G5-G6).en
dc.description.affiliationUNESP,FAC CIENCIAS & LETRAS ASSIS,DEPT CIENCIAS BIOL,AV DOM ANTONIO 2100,CAMPUS UNIV,BR-19800000 ASSIS,SP,BRAZIL
dc.description.affiliationUnespUNESP,FAC CIENCIAS & LETRAS ASSIS,DEPT CIENCIAS BIOL,AV DOM ANTONIO 2100,CAMPUS UNIV,BR-19800000 ASSIS,SP,BRAZIL
dc.format.extent101-105
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_issues&pid=0001-3714&lng=en&nrm=iso
dc.identifier.citationRevista de Microbiologia. São Paulo: Soc Brasileira Microbiologia, v. 28, n. 2, p. 101-105, 1997.
dc.identifier.issn0001-3714
dc.identifier.urihttp://hdl.handle.net/11449/37959
dc.identifier.wosWOS:A1997XU75900007
dc.language.isoeng
dc.publisherSoc Brasileira Microbiologia
dc.relation.ispartofRevista de Microbiologia
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectalpha-amylasept
dc.subjectRhizopus sppt
dc.subjectinductionpt
dc.subjectstarchpt
dc.subjectamylopectinpt
dc.subjectamylosept
dc.titleRelationship between carbon source, production and pattern action of alpha-amilase from Rhizopus spen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/rm/paboutj.htm
dcterms.rightsHolderSoc Brasileira Microbiologia
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências e Letras, Assispt
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Artigos - Ciências Biológicas - FCLAS