Perspectives on and Precautions for the Uses of Electric Spectroscopic Methods in Label-free Biosensing Applications

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dc.contributor.authorGarrote, Beatriz L. [UNESP]
dc.contributor.authorSantos, Adriano [UNESP]
dc.contributor.authorBueno, Paulo R. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2019-10-06T17:19:33Z
dc.date.available2019-10-06T17:19:33Z
dc.date.issued2019-01-01
dc.description.abstractLabel-free approaches for molecular diagnostic applications are appealing because of their inherent point-of-care advantages. Nonetheless, technical challenges impose a limit on the use of these methods as will be discussed in this paper. Electrochemical spectroscopic methods, such as impedance and impedance-derived methods, are highly effective in the development of label-free diagnostic assays, but they require careful control of the dynamics of the sensing interface. We herein report the strength and challenges of the current methodologies associated with the applications of impedance and impedance-derived methods by focusing on their principles of operation. We demonstrate that the uses of their potentialities are not based on the know-how of these methods, but on how to combine the spectroscopic features with the required chemical design for the associated sensing interfaces. Predominantly, we illustrate how to use the resistive and capacitive terms of the interface to improve its sensitivity to the target. For instance, with the proper signal amplification strategy, limitations related to target-to-receptor size ratio can be overcome. The target-to-receptor ratio is one of the difficulties that we use as an example to illustrate how the sensing of an electric signal can be improved by controlling the properties of the interface on the nanometer scale.en
dc.description.affiliationInstitute of Chemistry São Paulo State University (UNESP Universidade Estadual Paulista), 55 Prof. Francisco Degni st
dc.description.affiliationUnespInstitute of Chemistry São Paulo State University (UNESP Universidade Estadual Paulista), 55 Prof. Francisco Degni st
dc.identifierhttp://dx.doi.org/10.1021/acssensors.9b01177
dc.identifier.citationACS Sensors.
dc.identifier.doi10.1021/acssensors.9b01177
dc.identifier.issn2379-3694
dc.identifier.scopus2-s2.0-85071731514
dc.identifier.urihttp://hdl.handle.net/11449/190628
dc.language.isoeng
dc.relation.ispartofACS Sensors
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectbiosensors
dc.subjectcapacitive biosensors
dc.subjectcharge transfer resistance
dc.subjectelectrochemical capacitance
dc.subjectelectrochemical impedance spectroscopy
dc.subjectlabel-free diagnostic
dc.subjectmolecular assaying
dc.subjectpoint-of-care diagnostics
dc.titlePerspectives on and Precautions for the Uses of Electric Spectroscopic Methods in Label-free Biosensing Applicationsen
dc.typeArtigo
unesp.author.orcid0000-0003-2827-0208[3]

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Artigos - Físico Química - IQ