A fast and cheap in-house magnetic bead RNA extraction method for COVID-19 diagnosis

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dc.contributor.authorPossebon, Fabio Sossai [UNESP]
dc.contributor.authorUllmann, Leila Sabrina [UNESP]
dc.contributor.authorMalossi, Camila Dantas [UNESP]
dc.contributor.authorMiodutzki, Gabrielle Thaís [UNESP]
dc.contributor.authorda Silva, Evelyn Cristine [UNESP]
dc.contributor.authorMachado, Eduardo Ferreira [UNESP]
dc.contributor.authorBraga, Iolanda Simões [UNESP]
dc.contributor.authorPelaquim, Isadora Fernanda [UNESP]
dc.contributor.authorAraujo, João Pessoa [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-29T08:37:21Z
dc.date.available2022-04-29T08:37:21Z
dc.date.issued2022-02-01
dc.description.abstractCOVID-19 has posed a worldwide public health challenge affecting millions of people in different countries. Rapid and efficient detection of SARS-CoV-2 is essential for pandemic control. Reverse Transcription quantitative PCR (RT-qPCR) of nasopharyngeal swabs is the gold standard method for the virus detection, but the high demand for tests has substantially increased the costs and reduced the availability of reagents, including genetic material purification kits. Thus, the present study aimed to compare two bead-based RNA extraction methods (an in-house and a commercial kit) from nasopharyngeal swabs and RT-qPCR detection of SARS-CoV-2. Twenty-five positive and five negative nasopharyngeal swab samples were subjected to extraction of nucleic acids using both methods in an automated platform. Both protocols revealed a high correlation between Cycle Quantifications (Cqs) (r = 0.99, p < 0.0001). In addition, the in-house kit was 89.5 % cheaper when compared to the mean cost of commercial RNA extraction kits. The results show that the in-house protocol is an affordable and reliable option for RNA extraction for SARS-CoV-2 detection from nasopharyngeal swabs.en
dc.description.affiliationSão Paulo State University (UNESP) Institute of Biotechnology (IBTEC)
dc.description.affiliationSão Paulo State University (UNESP) Department of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Science
dc.description.affiliationSão Paulo State University (UNESP) Department of Veterinary Clinics School of Veterinary Medicine and Animal Science
dc.description.affiliationUnespSão Paulo State University (UNESP) Institute of Biotechnology (IBTEC)
dc.description.affiliationUnespSão Paulo State University (UNESP) Department of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Science
dc.description.affiliationUnespSão Paulo State University (UNESP) Department of Veterinary Clinics School of Veterinary Medicine and Animal Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 19/18581-6
dc.identifierhttp://dx.doi.org/10.1016/j.jviromet.2021.114414
dc.identifier.citationJournal of Virological Methods, v. 300.
dc.identifier.doi10.1016/j.jviromet.2021.114414
dc.identifier.issn1879-0984
dc.identifier.issn0166-0934
dc.identifier.scopus2-s2.0-85121110711
dc.identifier.urihttp://hdl.handle.net/11449/230052
dc.language.isoeng
dc.relation.ispartofJournal of Virological Methods
dc.sourceScopus
dc.subjectCOVID-19 test
dc.subjectRNA extraction
dc.subjectRT-qPCR
dc.subjectSARS-CoV-2
dc.titleA fast and cheap in-house magnetic bead RNA extraction method for COVID-19 diagnosisen
dc.typeArtigo
unesp.author.orcid0000-0002-0118-6164 0000-0002-0118-6164[1]
unesp.author.orcid0000-0003-2135-9488[7]

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Artigos - Produção Animal - FMVZ
Artigos - Clínica Veterinária - FMVZ