Inhibition of the myotoxic activity of Bothrops jararacussu venom and its two major myotoxins, BthTX-I and BthTX-II, by the aqueous extract of Tabernaemontana catharinensis A. DC. (Apocynaceae)

Página do item simplificado
dc.contributor.authorVeronese, ELG
dc.contributor.authorEsmeraldino, L. E.
dc.contributor.authorTrombone, APF
dc.contributor.authorSantana, A. E.
dc.contributor.authorBechara, G. H.
dc.contributor.authorKettelhut, I
dc.contributor.authorCintra, ACO
dc.contributor.authorGiglio, JR
dc.contributor.authorSampaio, S. V.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:13:57Z
dc.date.available2014-05-20T13:13:57Z
dc.date.issued2005-01-01
dc.description.abstractPartial neutralization of the myotoxic effect of Bothrops jararacussu venom (BV) and two of its myotoxins [bothropstoxin-I (BthTX-I), catalytically inactive, and II (BthTX-II), showing low PLA(2) activity], by the lyophilized aqueous extract of Tabernaemontana catharinensis (AE), was studied in rat isolated soleus muscle preparations (in vitro) and through i.m. injection in the gastrocnemius muscle (in vivo) by determination of creatine kinase (CK) activity and histopathological analysis. Incubation of soleus muscle for 1 h with BV or toxins (20 mug/ml) plus AE (400 mug/ml) added immediately after BV, BthTX-I or BthTX-II reduced CK levels by 53%, 37% and 56%, respectively. The myonecrotic effects of BV (20 mug/ml) upon soleus muscle was reduced 24%, 35% and 36% when AE (400 mug/ml) was added 1 h after BV and CK was evaluated 30 min, 1 and 2 h later, respectively. For BthTX-I these values were 46%, 48% and 47%, while for BthTX-II no inhibitory effect was detected. Histological analysis of soleus muscle after incubation with AE (400 mug/ml, I h) did not reveal any change in muscle fibers, but severe necrosis induced by -BV or toxins (20 mug/ml) was clearly in evidence, and decreased significantly when soleus muscle was protected by AE. This protection was also observed when AE was administered 1 h after BV or BthTX-I, but not after BthTX-II. AE did not inhibit the catalytic PLA(2), activity of BthTX-II or BV and did not change the PAGE pattern of BV, BthTX-I or BthTX-II. In vivo assays were performed in 100-g rats and maximal CK release was attained at a dose of 100 mug of BV, 3 h after injection. AE was not effective when injected 20 s after BV or toxins. However, injecting BV or toxins (100 mug), which were pre-incubated with AE (2 mg) caused an inhibition of 57%, 59% and 51%, respectively, with zero time pre-incubation, but was less effective with I h pre-incubation. This plant represents a potential source of promising myotoxin inhibitors. (C) 2004 Elsevier GmbH. All rights reserved.en
dc.description.affiliationUniv São Paulo, Fac Ciências Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14010903 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Clin & Cirurgia Vet, Fac Ciências Agrarias & Vet Jaboticabal, BR-14870000 Jaboticabal, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Patol Vet, Fac Ciências Agrarias & Vet Jaboticabal, BR-14870000 Jaboticabal, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Bioquim & Imunol, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Clin & Cirurgia Vet, Fac Ciências Agrarias & Vet Jaboticabal, BR-14870000 Jaboticabal, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Patol Vet, Fac Ciências Agrarias & Vet Jaboticabal, BR-14870000 Jaboticabal, SP, Brazil
dc.format.extent123-130
dc.identifierhttp://dx.doi.org/10.1016/j.phymed.2003.07.010
dc.identifier.citationPhytomedicine. Jena: Urban & Fischer Verlag, v. 12, n. 1-2, p. 123-130, 2005.
dc.identifier.doi10.1016/j.phymed.2003.07.010
dc.identifier.issn0944-7113
dc.identifier.lattes8905659987198068
dc.identifier.lattes2382374201685423
dc.identifier.urihttp://hdl.handle.net/11449/1579
dc.identifier.wosWOS:000227112800019
dc.language.isoeng
dc.publisherUrban & Fischer Verlag
dc.relation.ispartofPhytomedicine
dc.relation.ispartofjcr3.610
dc.relation.ispartofsjr1,087
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectphospholipase A(2)pt
dc.subjectmyotoxinspt
dc.subjectBothrops jararacussu venompt
dc.subjectbothropstoxinspt
dc.subjectTabernaemontana catharinensispt
dc.titleInhibition of the myotoxic activity of Bothrops jararacussu venom and its two major myotoxins, BthTX-I and BthTX-II, by the aqueous extract of Tabernaemontana catharinensis A. DC. (Apocynaceae)en
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderUrban & Fischer Verlag
unesp.author.lattes8905659987198068[4]
unesp.author.lattes2382374201685423
unesp.author.orcid0000-0003-4619-3744[5]
unesp.author.orcid0000-0002-8102-6098[3]
unesp.author.orcid0000-0003-3274-180X[9]
unesp.author.orcid0000-0003-4539-1802[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
unesp.departmentClínica e Cirurgia Veterinária - FCAVpt
unesp.departmentPatologia Veterinária - FCAVpt

Arquivos

Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias