Influence of the collection methodology on the Apis mellifera venom composition: Peptide analysis

dc.contributor.authorBrochetto-Braga, Márcia Regina [UNESP]
dc.contributor.authorPalma, Mario Sergio [UNESP]
dc.contributor.authorCarmona, Eleonora Cano [UNESP]
dc.contributor.authorChaud-Netto, José [UNESP]
dc.contributor.authorRodrigues, Anderson [UNESP]
dc.contributor.authorDa Silva, Giselly Pereira [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:21:53Z
dc.date.available2014-05-27T11:21:53Z
dc.date.issued2006-06-07
dc.description.abstractDifferences of venom peptide composition as function of two collection methodologies, electrical stimulation (ES) and reservoir disruption (RD), were analyzed by reverse-phase HPLC in Apis mellifera races - A. m. adansonii, A. m. ligustica and Africanized honeybee. The analyses were performed through determination of the relative number and percentage of each molecular form associated to the peaks eluted by chromatography. Comparison of these profiles revealed qualitative and quantitative differences related to the venom collection methodology as well to the three races analyzed. In contrast to data usually found for venom proteins, the three races presented a major number of peaks or molecular forms when venom was collected by ES. Besides, in general, the relative concentration of each peak was higher for ES in relation to RD. That indicates the presence of molecular precursors in the venom obtained by RD. The presence/absence pattern of the peaks, such as their relative concentrations showed a closer similarity between A. m. adansonii and the Africanized honeybees than that observed between these and A. m. ligustica. The obtained data allowed a discussion about the differences in the relative concentration of each venom component according to the collection methodology, and finally the biological action of the venom in different races. So, these results, apart from being useful to establish a peptide profile for each bee race as a function of the venom collection methodology, pointed out once more that the chromatographic techniques are a great tool for the identification of A. mellifera subspecies.en
dc.description.affiliationDepto. de Biologia Instituto de Biociências UNESP, Av. 24A, CEP 13506-900, Rio Claro, SP
dc.description.affiliationDepto. de Bioquímica e Microbiologia Instituto de Biociências UNESP, Av. 24A, CEP 13506-900, Rio Claro, SP
dc.description.affiliationUnespDepto. de Biologia Instituto de Biociências UNESP, Av. 24A, CEP 13506-900, Rio Claro, SP
dc.description.affiliationUnespDepto. de Bioquímica e Microbiologia Instituto de Biociências UNESP, Av. 24A, CEP 13506-900, Rio Claro, SP
dc.format.extent759-770
dc.identifierhttp://periodicos.uefs.br/ojs/index.php/sociobiology/issue/archive
dc.identifier.citationSociobiology, v. 47, n. 3, p. 759-770, 2006.
dc.identifier.issn0361-6525
dc.identifier.lattes2901888624506535
dc.identifier.lattes4110421764783871
dc.identifier.scopus2-s2.0-33744527805
dc.identifier.urihttp://hdl.handle.net/11449/68914
dc.identifier.wosWOS:000237498800009
dc.language.isoeng
dc.relation.ispartofSociobiology
dc.relation.ispartofjcr0.604
dc.relation.ispartofsjr0,396
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAfricanized bees
dc.subjectApis mellifera
dc.subjectHoneybee races
dc.subjectPeptides
dc.subjectVenom collection
dc.subjectVenom composition
dc.subjectApoidea
dc.titleInfluence of the collection methodology on the Apis mellifera venom composition: Peptide analysisen
dc.typeArtigo
dcterms.licensehttp://periodicos.uefs.br/ojs/index.php/sociobiology/about/submissions#copyrightNotice
unesp.author.lattes2901888624506535
unesp.author.lattes4110421764783871
unesp.author.orcid0000-0002-0230-5735[3]
unesp.author.orcid0000-0002-7982-0743[1]
unesp.author.orcid0000-0002-7363-8211[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt
unesp.departmentBiologia - IBpt
unesp.departmentBioquímica e Microbiologia - IBpt

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