A case study in flux balance analysis: Lysine, a cephamycin C precursor, can also increase clavulanic acid production

dc.contributor.authorCavallieri, André Pastrelo [UNESP]
dc.contributor.authorBaptista, Amanda Salvador [UNESP]
dc.contributor.authorLeite, Carla Andréa [UNESP]
dc.contributor.authorAraujo, Maria Lucia Gonsales da Costa [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:02:15Z
dc.date.available2018-12-11T17:02:15Z
dc.date.issued2016-08-15
dc.description.abstractStreptomyces clavuligerus is an excellent model for studying complex relationships involved in the biosynthesis of secondary metabolites. Despite the fact that several studies have investigated the mechanisms pertaining to the biosynthesis of two bioactive compounds produced by S. clavuligerus, cephamycin C (CephC) and clavulanic acid (CA), few have focused on metabolic flux analyses of this microorganism. It was proposed a novel representative metabolic model for S. clavuligerus addressing the simultaneous production of CephC and CA using flux balance analysis methodology (FBA). Although both are synthesized via different pathway, flux analysis showed that the production of these bioactives are connected. CephC production showed high dependency on N metabolism while CA production is more related with C metabolism. Adding lysine plus maltose increased CA, an interesting outcome since lysine is a CephC precursor. Notwithstanding, it was possible to propose a mechanism for this behavior by means of flux analysis. Furthermore, the results suggest that ATP yield maximization is the objective function that best suits the conditions under investigation.en
dc.description.affiliationUNESP-São Paulo State University Institute of Chemistry Department of Biochemistry and Technological Chemistry
dc.description.affiliationUnespUNESP-São Paulo State University Institute of Chemistry Department of Biochemistry and Technological Chemistry
dc.format.extent42-53
dc.identifierhttp://dx.doi.org/10.1016/j.bej.2016.03.012
dc.identifier.citationBiochemical Engineering Journal, v. 112, p. 42-53.
dc.identifier.doi10.1016/j.bej.2016.03.012
dc.identifier.issn1873-295X
dc.identifier.issn1369-703X
dc.identifier.lattes2478051365107560
dc.identifier.scopus2-s2.0-84962911719
dc.identifier.urihttp://hdl.handle.net/11449/172808
dc.language.isoeng
dc.relation.ispartofBiochemical Engineering Journal
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAntibiotic
dc.subjectFilamentous bacteria
dc.subjectFlux balance analysis
dc.subjectGrowth kinetics
dc.subjectLysine
dc.subjectPhysiology
dc.titleA case study in flux balance analysis: Lysine, a cephamycin C precursor, can also increase clavulanic acid productionen
dc.typeArtigo
unesp.author.lattes2478051365107560

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