Chemoprotective activity of the isoflavones, genistein and daidzein on mutagenicity induced by direct and indirect mutagens in cultured HTC cells

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dc.contributor.authorLepri, Sandra Regina
dc.contributor.authorLuiz, Rodrigo Cabral
dc.contributor.authorZanelatto, Leonardo Campos
dc.contributor.authorDa Silva, Patrícia Benites Gonçalves
dc.contributor.authorSartori, Daniele
dc.contributor.authorRibeiro, Lucia Regina [UNESP]
dc.contributor.authorMantovani, Mario Sergio
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:28:33Z
dc.date.available2014-05-27T11:28:33Z
dc.date.issued2013-03-01
dc.description.abstractIsoflavones are phenolic compounds widely distributed in plants and found in a high percentage in soybeans. They have important biological properties and are regarded as potential chemopreventive agents. The aim of this study was to verify the preventive effect of two soy isoflavones (genistein and daidzein) by a micronucleus assay, analysis of GST activity, and real-time RT-PCR analysis of GSTa2 gene expression. Mutagens of direct (doxorubicin) and indirect (2-aminoanthracene) DNA damage were used. Hepatoma cells (HTC) were treated with genistein or daidzein for 26 h at noncytotoxic concentrations; 10 μM when alone, and 0.1, 1.0 and 10 μM when combined with genotoxic agents. The micronucleus test demonstrated that both isoflavones alone had no genotoxic effect. Genistein showed antimutagenic effects at 10 μM with both direct and indirect DNA damage agents. On phase II enzyme regulation, the current study indicated an increase in total cytoplasmic GST activity in response to genistein and daidzein at 10 μM supplementation. However, the mRNA levels of GSTa2 isozymes were not differentially modulated by genistein or daidzein. The results point to an in vitro antimutagenic activity of genistein against direct and indirect DNA damage-induced mutagenicity. © 2012 Springer Science+Business Media B.V.en
dc.description.affiliationGeneral Biology Department State University of Londrina (UEL) Campus Universitário, Rodovia Celso Garcia Cid, Pr 445 km 380, Cx. Postal 6001, Londrina, PR CEP 86051-980
dc.description.affiliationPathological Sciences Department State University of Londrina (UEL), Londrina, PR
dc.description.affiliationInstitute of Biosciences UNESP, Rio Claro, SP
dc.description.affiliationUnespInstitute of Biosciences UNESP, Rio Claro, SP
dc.format.extent213-222
dc.identifierhttp://dx.doi.org/10.1007/s10616-012-9476-8
dc.identifier.citationCytotechnology, v. 65, n. 2, p. 213-222, 2013.
dc.identifier.doi10.1007/s10616-012-9476-8
dc.identifier.issn0920-9069
dc.identifier.issn1573-0778
dc.identifier.scopus2-s2.0-84878362110
dc.identifier.urihttp://hdl.handle.net/11449/74642
dc.identifier.wosWOS:000314310000006
dc.language.isoeng
dc.relation.ispartofCytotechnology
dc.relation.ispartofjcr1.461
dc.relation.ispartofsjr0,519
dc.relation.ispartofsjr0,519
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAntimutagenicity
dc.subjectDaidzein
dc.subjectGenistein
dc.subjectHepatoma cell line
dc.subjectPhase II enzyme
dc.subject2 aminoanthracene
dc.subjectdaidzein
dc.subjectDNA
dc.subjectdoxorubicin
dc.subjectgenistein
dc.subjectglutathione transferase
dc.subjectglutathione transferase A2
dc.subjectisoflavone
dc.subjectmutagenic agent
dc.subjectantineoplastic activity
dc.subjectcell culture
dc.subjectchemoprophylaxis
dc.subjectcytotoxicity
dc.subjectDNA damage
dc.subjectenzyme activity
dc.subjectenzyme regulation
dc.subjectgenotoxicity
dc.subjecthepatoma cell
dc.subjectmutagen testing
dc.subjectmutagenicity
dc.subjectnucleotide sequence
dc.subjectreal time polymerase chain reaction
dc.subjectGlycine max
dc.titleChemoprotective activity of the isoflavones, genistein and daidzein on mutagenicity induced by direct and indirect mutagens in cultured HTC cellsen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights

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