Evaluation of a highly sensitive amperometric biosensor with low cholinesterase charge immobilized on a chemically modified carbon paste electrode for trace determination of carbamates in fruit, vegetable and water samples

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dc.contributor.authorNunes, G. S S
dc.contributor.authorBarcelo, D.
dc.contributor.authorGrabaric, B. S.
dc.contributor.authorDiaz-Cruz, J. M.
dc.contributor.authorRibeiro, M. L.
dc.contributor.institutionUniversidade Federal do Maranhão (UFMA)
dc.contributor.institutionCSIC
dc.contributor.institutionUniv Barcelona
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:25:05Z
dc.date.available2014-05-20T15:25:05Z
dc.date.issued1999-11-08
dc.description.abstractA highly sensitive amperometric biosensor for determination of carbamate pesticides directly in water, fruit and vegetable samples has been evaluated, electrochemically characterized and optimized. The biosensor strip was fabricated in screen printed technique on a ceramic support using silver-based paste for reference electrode, and platinum-based paste for working and auxiliary electrodes. The working electrode was modified by a layer of carbon paste mixed with cobalt(II) phthalocyanine and acetylcellulose. Cholinesterase (ChE) enzymes with low enzymatic charge were immobilized on this layer. The operational simplicity of the biosensor consists in that a small drop (similar to 50 mu l) of substrate or sample is deposited on a horizontally positioned biosensor strip representing the microelectrochemical cell. The working potential of the biosensor was 370 mV versus Ag/AgI on a ship reference electrode preventing the interference of electroactive species which are oxidable at more positive potentials. The biosensor was applied to investigate the degradation of two reference ChE inhibitors in freeze dried water under different storage conditions and for direct determination of some N-methylcarbamates (NMCs) in fruit and vegetable samples at ppb concentration levels without any sample pretreatment. A comparison of the obtained results for the total carbamate concentration was done against those obtained using HPLC measurements. (C) 1999 Elsevier B.V. B.V. All rights reserved.en
dc.description.affiliationFed Univ Maranhao, UFMA, Dept Tech Chem, BR-65080040 Sao Luis Ma, Brazil
dc.description.affiliationCSIC, Cid, Dept Environm Chem, Barcelona 08040, Spain
dc.description.affiliationUniv Barcelona, Dept Analyt Chem, E-08028 Barcelona, Spain
dc.description.affiliationUNESP, Inst Chem, BR-14800900 Araraquara, SP, Brazil
dc.description.affiliationUnespUNESP, Inst Chem, BR-14800900 Araraquara, SP, Brazil
dc.format.extent37-49
dc.identifierhttp://dx.doi.org/10.1016/S0003-2670(99)00574-7
dc.identifier.citationAnalytica Chimica Acta. Amsterdam: Elsevier B.V., v. 399, n. 1-2, p. 37-49, 1999.
dc.identifier.doi10.1016/S0003-2670(99)00574-7
dc.identifier.issn0003-2670
dc.identifier.urihttp://hdl.handle.net/11449/35553
dc.identifier.wosWOS:000083467100006
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofAnalytica Chimica Acta
dc.relation.ispartofjcr5.123
dc.relation.ispartofsjr1,512
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectamperometric biosensorpt
dc.subjectcholinesterase-based biosensorpt
dc.subjectHPLC analysispt
dc.subjectcarbamatespt
dc.subjectfruit samplespt
dc.subjectvegetable samplespt
dc.titleEvaluation of a highly sensitive amperometric biosensor with low cholinesterase charge immobilized on a chemically modified carbon paste electrode for trace determination of carbamates in fruit, vegetable and water samplesen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0002-8873-0491[2]

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