3D printed geopolymer: An efficient support for immobilization of Candida rugosa lipase

dos Santos, Letícia Karen [UNESP]; Botti, Renata Fuss; Innocentini, Murilo Daniel de Mello; Marques, Rodrigo Fernando Costa [UNESP]; Colombo, Paolo; de Paula, Ariela Veloso [UNESP]; Flumignan, Danilo Luiz [UNESP]

3D printed geopolymer: An efficient support for immobilization of Candida rugosa lipase

Data

2021-06-15

Autores

dos Santos, Letícia Karen

Botti, Renata Fuss

Innocentini, Murilo Daniel de Mello

Marques, Rodrigo Fernando Costa

Colombo, Paolo

de Paula, Ariela Veloso

Flumignan, Danilo Luiz

Tipo

Artigo

Resumo

Lattice-shaped geopolymers were successfully prepared by Direct Ink Writing to act as carriers for the immobilization of Candida rugosa lipase (CRL). The supported biocatalyst was evaluated in the hydrolysis of waste cooking oil (WCO), a preliminary step for the production of biodiesel. The lattice samples presented total and open porosities of 57.2 vol% and 56.4 vol% respectively, bulk density of 0.924 ± 0.059 g/cm3, and true density of struts of 2.157 ± 0.014 g/cm3. The permeability coefficients (k1 = (9.05 ± 0.41) × 10−9 m2 and k2 = (3.64 ± 0.26) × 10−4 m) were consistently higher than those of typical enzymatic carriers. The geopolymer surface was successfully modified to allow the immobilization process by covalent bonding of CRL. The hydrolytic activity reached 847.7 ± 9.7 U/g. A free fatty acids content of 75 wt% was achieved from the hydrolysis of WCO, proving the efficiency of immobilization and the suitability of lattice-shaped geopolymers as support for biocatalysts.