Beta-hydroxy-beta-methylbutyrate associated with low-intensity exercise training improves skeletal muscle regeneration through the IGF-Akt pathway

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dc.contributor.authorYamada, A. K.
dc.contributor.authorFerretti, R. [UNESP]
dc.contributor.authorMatsumura, C. Y. [UNESP]
dc.contributor.authorAntunes, L.
dc.contributor.authorda Silva, C. A.
dc.contributor.authorPertille, A.
dc.contributor.institutionUniversidade Metodista de Piracicaba
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Paulista-Swift
dc.date.accessioned2022-04-28T19:50:06Z
dc.date.available2022-04-28T19:50:06Z
dc.date.issued2022-01-01
dc.description.abstractThe effect of beta-hydroxy-beta-methylbutyrate (HMB) supplementation associated with exercise training at different intensities and frequencies on skeletal muscle regeneration of muscle-injured rats was investigated. Male Wistar rats were divided into sedentary and trained groups. The sedentary groups were subdivided into non-injured (SED-Ct), non-injured supplemented with HMB (SED-Ct-HMB), injured (SED), and injured with HMB (SED-HMB), and the trained groups were injured, supplemented with HMB, and then divided into training three times a week without load (HT3) or with load (HT3L) and training five times a week without load (HT5) and with load (HT5L). The rats received a daily dose of HMB associated with 60 min of swimming with or without 5% body mass load for 14 days. On the 15th day, cryoinjury was performed in the right tibialis anterior muscle (TA), and 48 h later, supplementation and training continued for 15 days. After the last session, the TA was dissected and a cross-sectional area (CSA) of muscle fibers was used to determine the percentage of CSA fibers and connective tissue (%CT), as well as the total and phosphorylated protein contents. SED-HMB showed increased CSA and decreased %CT and TGF-b when compared to SED. HT3 showed increased CSA and reduced %CT accompanied by increased IGF-1/Akt, myogenin, and MuRF1, and decreased TGF-b. The CSA of HT5L also increased, but at the cost of a higher %CT compared to the other groups. Our results demonstrated that HMB associated with training without load and with lower frequency per week may be a valuable strategy for skeletal muscle regeneration.en
dc.description.affiliationPrograma de Pós-Graduac¸ão em Ciências do Movimento Humano Laboratório de Plasticidade Neuromuscular Universidade Metodista de Piracicaba, SP
dc.description.affiliationDepartamento de Biologia Estrutural e Funcional Instituto de Biociências de Botucatu Universidade Estadual Paulista, SP
dc.description.affiliationInstituto de Ciências da Saúde Faculdade de Ciências da Saúde Universidade Paulista-Swift, SP
dc.description.affiliationUnespDepartamento de Biologia Estrutural e Funcional Instituto de Biociências de Botucatu Universidade Estadual Paulista, SP
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCAPES: 001
dc.description.sponsorshipIdCNPq: 423505/2016-5
dc.identifierhttp://dx.doi.org/10.1590/1414-431X2021e11597
dc.identifier.citationBrazilian Journal of Medical and Biological Research, v. 55.
dc.identifier.doi10.1590/1414-431X2021e11597
dc.identifier.issn1678-4510
dc.identifier.issn0100-879X
dc.identifier.scopus2-s2.0-85123560490
dc.identifier.urihttp://hdl.handle.net/11449/223341
dc.language.isoeng
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.sourceScopus
dc.subjectExercise
dc.subjectInjury
dc.subjectMuscle
dc.subjectRegeneration
dc.subjectSignaling pathways
dc.subjectSupplementation
dc.titleBeta-hydroxy-beta-methylbutyrate associated with low-intensity exercise training improves skeletal muscle regeneration through the IGF-Akt pathwayen
dc.typeArtigo

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