Production and Characterization of β-glucosidase Obtained by the Solid-State Cultivation of the Thermophilic Fungus Thermomucor indicae-seudaticae N31

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dc.contributor.authorde Cassia Pereira, Josiani [UNESP]
dc.contributor.authorLeite, Rodrigo Simões Ribeiro
dc.contributor.authordo Prado, Heloiza Ferreira Alves [UNESP]
dc.contributor.authorBocchini Martins, Daniela Alonso [UNESP]
dc.contributor.authorGomes, Eleni [UNESP]
dc.contributor.authorda Silva, Roberto [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal da Grande Dourados (UFGD)
dc.date.accessioned2021-06-25T11:03:57Z
dc.date.available2021-06-25T11:03:57Z
dc.date.issued2015-01-01
dc.description.abstractIn this paper, several agro-industrial wastes (soybean meal and wheat straw, rice and peanut husks, corn cob and corn stover, and sugarcane bagasse) were tested for the production of β-glucosidase by the cultivation of thermophilic fungus Thermomucor indicae-seudaticae N31 in solid-state fermentation (SSF). Among the tested substrates, the highest yields were obtained in soybean meal. Other fermentation parameters were also evaluated, such as initial pH, merge substrates, and fermentation time, as well as the physicochemical characterization of the enzyme. The best results were obtained after 192 h of fermentation with the initial pH adjusted to 6.0. The substrate mixture did not improve the enzyme production by microorganism. The β-glucosidase showed best catalytic activity at pH 4.5 and at 75 °C and remained stable in the pH range from 4.5 to 9.5 and the temperature range 40–75 °C. The enzyme showed 80 % of its activity at a concentration of 15 mM glucose and remained stable up to 20 % ethanol.en
dc.description.affiliationLaboratory of Biochemistry and Applied Microbiology Universidade Estadual Paulista (UNESP), Av. Cristóvão Colombo, 2265
dc.description.affiliationBiological na Environment Sciences Faculty FCBA Universidade Federal da Grande Dourados (UFGD), Km 12, Rodovia Dourados—Itahum
dc.description.affiliationUnespLaboratory of Biochemistry and Applied Microbiology Universidade Estadual Paulista (UNESP), Av. Cristóvão Colombo, 2265
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent723-732
dc.identifierhttp://dx.doi.org/10.1007/s12010-014-1332-1
dc.identifier.citationApplied Biochemistry and Biotechnology, v. 175, n. 2, p. 723-732, 2015.
dc.identifier.doi10.1007/s12010-014-1332-1
dc.identifier.issn1559-0291
dc.identifier.issn0273-2289
dc.identifier.scopus2-s2.0-84921059045
dc.identifier.urihttp://hdl.handle.net/11449/207957
dc.language.isoeng
dc.relation.ispartofApplied Biochemistry and Biotechnology
dc.sourceScopus
dc.subjectSolid-state fermentation
dc.subjectThermomucor indicae-seudaticae N31
dc.subjectβ-glucosidase
dc.titleProduction and Characterization of β-glucosidase Obtained by the Solid-State Cultivation of the Thermophilic Fungus Thermomucor indicae-seudaticae N31en
dc.typeArtigo

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