Influence of glycerol and ornithine feeding on clavulanic acid production by streptomyces clavuligerus

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dc.contributor.authorTeodoro, J. C.
dc.contributor.authorBaptista-Neto, A.
dc.contributor.authorAraujo, M. L.G.C. [UNESP]
dc.contributor.authorHokka, C. O.
dc.contributor.authorBadino, A. C.
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-28T22:02:08Z
dc.date.available2022-04-28T22:02:08Z
dc.date.issued2010-01-01
dc.description.abstractThe influence of glycerol and ornithine feeding on clavulanic acid (CA) production by Streptomyces clavuligerus was investigated. In batch experiments, CA maximum concentration (Cpmax) ranged randomly from 430 to 560 mg.L-1, with a maximum increase of 10% in relation to the control run, without ornithine. However, the maximum volumetric productivity of CA (Ppmax) of 13.7 mg.L-1.h-1 was obtained with 0.66 g.L-1 of ornithine, 44.2% higher than the Ppmax in the control run. In fed-batch experiments, Cpmax varied within the narrow range from 1.254 to 1.405 g.L-1, 2.5 times higher than that obtained in the control run. The presence of ornithine increased the Pp max, although it influenced only slightly the Cpmax. Concerning glycerol, the highest CA production of 1.6 g.L-1 was obtained in the fed-batch with glycerol and ornithine (180 and 3.7 g.L -1) in a 10-L bioreactor, showing a positive effect of ornithine and glycerol, in the proper proportion (48.6:1), on CA biosynthesis.en
dc.description.affiliationUFSCar Federal University of São Carlos Department of Chemical Engineering, Rod. Washington Luiz, 13565-905, São Carlos - SP
dc.description.affiliationUNESP Institute of Chemistry Department of Biochemistry and Technological Chemistry, Rua Prof. Francisco Degni s/n, 14800-900, Araraquara - SP
dc.description.affiliationUnespUNESP Institute of Chemistry Department of Biochemistry and Technological Chemistry, Rua Prof. Francisco Degni s/n, 14800-900, Araraquara - SP
dc.format.extent499-506
dc.identifierhttp://dx.doi.org/10.1590/S0104-66322010000400001
dc.identifier.citationBrazilian Journal of Chemical Engineering, v. 27, n. 4, p. 499-506, 2010.
dc.identifier.doi10.1590/S0104-66322010000400001
dc.identifier.issn0104-6632
dc.identifier.scopus2-s2.0-78651481520
dc.identifier.urihttp://hdl.handle.net/11449/226205
dc.language.isoeng
dc.relation.ispartofBrazilian Journal of Chemical Engineering
dc.sourceScopus
dc.subjectClavulanic acid
dc.subjectFed-batch
dc.subjectGlycerol
dc.subjectOrnithine
dc.subjectStreptomyces clavuligerus
dc.titleInfluence of glycerol and ornithine feeding on clavulanic acid production by streptomyces clavuligerusen
dc.typeArtigo
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt
unesp.departmentBioquímica e Tecnologia - IQpt

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