Boa.PLI: Structural and functional characterization of the gamma phospholipase A2 plasma inhibitor from the non-venomous Brazilian snake Boa constrictor

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dc.contributor.authorBittencourt Rodrigues, Caroline Fabri
dc.contributor.authorSerino-Silva, Caroline
dc.contributor.authorMorais-Zani, Karen de
dc.contributor.authorKavazoi, Victor Koiti
dc.contributor.authorNogueira Carvalho, Marcelo Pires
dc.contributor.authorGrego, Kathleen Fernandes
dc.contributor.authorChiarelli, Tassia
dc.contributor.authorTashima, Alexandre Keiji
dc.contributor.authorToyama, Marcos Hikari [UNESP]
dc.contributor.authorTanaka-Azevedo, Anita Mitico
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionInst Butantan
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2020-12-10T20:00:06Z
dc.date.available2020-12-10T20:00:06Z
dc.date.issued2020-02-27
dc.description.abstractPlasma in several organisms has components that promote resistance to envenomation by inhibiting specific proteins from snake venoms, such as phospholipases A(2) (PLA(2)s). The major hypothesis for inhibitor's presence would be the protection against self-envenomation in venomous snakes, but the occurrence of inhibitors in non-venomous snakes and other animals has opened new perspectives for this molecule. Thus, this study showed for the first time the structural and functional characterization of the PLA(2) inhibitor from the Boa constrictor serum (Boa.PLI), a non-venomous snake that dwells extensively the Brazilian territory. Therefore, the inhibitor was isolated from B. constrictor serum, with 0.63% of recovery. SDS-PAGE showed a band at similar to 25 kDa under reducing conditions and similar to 20 kDa under non-reducing conditions. Chromatographic analyses showed the presence of oligomers formed by Boa.PLI. Primary structure of Boa.PLI suggested an estimated molecular mass of 22 kDa. When Boa.PLI was incubated with Asp-49 and Lys-49 PLA(2) there was no severe change in its dichroism spectrum, suggesting a non-covalent interaction. The enzymatic assay showed a dose-dependent inhibition, up to 48.2%, when Boa.PLI was incubated with Asp-49 PLA(2), since Lys-49 PLA(2) has a lack of enzymatic activity. The edematogenic and myotoxic effects of PLA(2)s were also inhibited by Boa.PLI. In summary, the present work provides new insights into inhibitors from non-venomous snakes, which possess PLIs in their plasma, although the contact with venom is unlikely.en
dc.description.affiliationUniv Sao Paulo, Inst Pesquisas Tecnol, Inst Butantan, Interunidades Biotecnol, Sao Paulo, SP, Brazil
dc.description.affiliationInst Butantan, Lab Herpetol, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Minas Gerais, Belo Horizonte, MG, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Bioquim, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias Litoral Paulista, Sao Vicente, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias Litoral Paulista, Sao Vicente, SP, Brazil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCAPES: 001
dc.description.sponsorshipIdFAPESP: 2017/16908-2
dc.description.sponsorshipIdFAPESP: 2017/20106-9
dc.description.sponsorshipIdFAPESP: 2018/25786-0
dc.format.extent13
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0229657
dc.identifier.citationPlos One. San Francisco: Public Library Science, v. 15, n. 2, 13 p., 2020.
dc.identifier.doi10.1371/journal.pone.0229657
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11449/196909
dc.identifier.wosWOS:000535237000056
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.sourceWeb of Science
dc.titleBoa.PLI: Structural and functional characterization of the gamma phospholipase A2 plasma inhibitor from the non-venomous Brazilian snake Boa constrictoren
dc.typeArtigo
dcterms.rightsHolderPublic Library Science

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Artigos - Ciências Biológicas - São Vicente