Fullerene and omega-3 and omega-6 fatty acids on fish brain antioxidant status

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dc.contributor.authorAcosta, Daiane da Silva
dc.contributor.authorKneip, Flavia Conde
dc.contributor.authorDe Almeida, Eduardo Alves [UNESP]
dc.contributor.authorVentura-Lima, Juliane
dc.contributor.authorMonserrat, Jose Maria
dc.contributor.authorGeracitano, Laura Alicia
dc.contributor.institutionUniversidade Federal do Rio Grande (FURG)
dc.contributor.institutionFundação Univ Fed Rio Grande
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T14:03:09Z
dc.date.available2014-05-20T14:03:09Z
dc.date.issued2012-10-01
dc.description.abstractThe carbon nanomaterial fullerene (C-60) can act as anti or pro-oxidant. The aim of this study was to evaluate, in cell suspensions of carp brains (Cyprinus carpio, Cyprinidae), the effect of C-60 after a pre-treatment with polyunsaturated fatty acid (PUFAs) such as omega-3 (docosahexaenoic acid, DHA) and omega-6 (linoleic acid, LA). Assays consisted of a pre-treatment with PUFA (48 h) and then exposure to C-60 (2 h). Cell viability and total anti-oxidant capacity did not differ (p > 0.05). A reduction (p < 0.05) was observed in reactive oxygen species (ROS) and thiobarbituric acid reactive substances (TBARS) concentration in fish brain cells pre-exposed with PUFA groups and then exposed or not with C-60. An antioxidant effect of C-60 was evident since in control group (cells not pre-exposed to PUFA), a significant (p < 0.05) reduction of intracellular ROS concentration was observed, although this reduction was not enough to reduce the TBARS levels. Cysteine levels presented a reduction (p < 0.05) in all groups exposed to C-60. For glutathione (GSH), an increase (p < 0.05) was registered in cells exposed to C-60 without PUFAs pre-treatment and in the C-60 group pre-treated with DHA. Overall C-60 appears to play an antioxidant role that is modulated by PUFA, taking into account its effects on intracellular ROS concentration and MDA levels. Results also suggest that C-60 influences GSH synthesis, as showed for the augmented levels of this antioxidant and also for the lowering of the intracellular cysteine concentration.en
dc.description.affiliationICB FURG, Inst Nacl Ciência & Tecnol Nanomat Carbono CNPq, BR-96201900 São Paulo, Brazil
dc.description.affiliationUniv Fed Rio Grande FURG, ICB, Rio Grande, RS, Brazil
dc.description.affiliationFundação Univ Fed Rio Grande, Programa Posgrad Ciencias Fisiol, Rio Grande, Brazil
dc.description.affiliationUniv Estadual Paulista, São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, São Paulo, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa no Estado do Rio Grande do Sul (FAPERGS)
dc.description.sponsorshipIdCNPq: 471459/2007-0
dc.description.sponsorshipIdFAPERGS: 10/0036-5-PRONEX/Conv. 700545/2008
dc.description.sponsorshipIdCNPq: 552131/2011-3
dc.format.extent1477-1485
dc.identifierhttp://dx.doi.org/10.1007/s10695-012-9635-z
dc.identifier.citationFish Physiology and Biochemistry. Dordrecht: Springer, v. 38, n. 5, p. 1477-1485, 2012.
dc.identifier.doi10.1007/s10695-012-9635-z
dc.identifier.issn0920-1742
dc.identifier.urihttp://hdl.handle.net/11449/22257
dc.identifier.wosWOS:000308968000022
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofFish Physiology and Biochemistry
dc.relation.ispartofjcr1.735
dc.relation.ispartofsjr0,690
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectFullereneen
dc.subjectPolyunsaturated acidsen
dc.subjectNanoparticlesen
dc.subjectFish brainen
dc.subjectCysteineen
dc.subjectGlutathioneen
dc.titleFullerene and omega-3 and omega-6 fatty acids on fish brain antioxidant statusen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer

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