Investigation of Toxoplasma gondii in semen, testicle and epididymis tissues of primo-infected cats (Felis catus)

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dc.contributor.authorTeixeira, Weslen Fabricio Pires [UNESP]
dc.contributor.authorTozato, Maria Eduarda Gonçalves [UNESP]
dc.contributor.authorPierucci, Julia Cestari [UNESP]
dc.contributor.authorVital, Giovana Pavão [UNESP]
dc.contributor.authorCruz, André Cayeiro [UNESP]
dc.contributor.authorLopes, Welber Daniel Zanetti [UNESP]
dc.contributor.authorCursino, Marina Suzuki [UNESP]
dc.contributor.authorJoaquim, Samea Fernandes [UNESP]
dc.contributor.authorSoares, Vando Edésio [UNESP]
dc.contributor.authorLangoni, Helio [UNESP]
dc.contributor.authorBresciani, Kátia Denise Saraiva [UNESP]
dc.contributor.authorCosta, Alvimar José da [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Goiás (UFG)
dc.contributor.institutionUniversidade Brasil – Campus Descalvado
dc.date.accessioned2018-12-11T17:11:12Z
dc.date.available2018-12-11T17:11:12Z
dc.date.issued2017-04-30
dc.description.abstractThis study aimed to investigate the presence of Toxoplasma gondii in semen, testicle and epididymis tissues of cats experimentally infected by this coccidium. A total of 12 male felines without a definite breed that were of reproductive age and serologically negative for T. gondii were selected and distributed to the following three experimental groups: GI, inoculated with 600 tissue cysts of the P strain of T. gondii (isolate III); GII, inoculated with 2 × 105 tachyzoites of the RH strain (isolate I); and GIII, not inoculated (control group). Prior to inoculation (day −7 and 0) and on post inoculation days (PIDs) 7, 14, 21, 28, 42, 56, and 70, all felines were subjected to assessments of anti-T. gondii IgG by indirect immunofluorescence (IIF) and assessments of parasitemia. Collection of semen (electroejaculation) was performed on the specified dates, followed by nested PCR and bioassays in mice to detect T. gondii. On PID 70, all 12 felines were orchiectomized, and the presence of the parasite in the testicles and epididymides was evaluated by nested PCR, murine bioassay, and histopathological and immunohistochemical analyses. All felines inoculated with T. gondii (GI and GII) seroconverted to the toxoplasmic infection after PID 14; on PID 7, seroconversion of three felines (P4, RH2 and RH4) could observed, and all exhibited detectable titers by PID 64. The GII felines exhibited greater serological titers compared with GI felines. The maximum serological titer (IgG) was observed in feline RH3 (titer 1024), while in other experimental felines, a maximum titer of 256 was detected. Parasitemic peaks were diagnosed in all felines of groups I and II from PIDs 7–42. A total of five parasitemic peaks were diagnosed in GI and nine in GII. In none of the experimental time points was the presence of T. gondii diagnosed in seminal samples collected from the felines or in the testicle or epididymis tissues collected from these animals. Thus, sexual transmission in domestic cats does not appear to be a major route of T. gondii infection, possibly demonstrating the tendency of this protozoan to develop a response directed to the formation and excretion of oocysts in the feces of these definite hosts, which act as its main route of perpetuation in the environment.en
dc.description.affiliationFaculdade de Ciências Agrárias e Veterinárias UNESP/CPPAR, Access route Prof. Paulo Donatto Castellane, no number, Jaboticabal, São Paulo
dc.description.affiliationInstituto de Patologia Tropical e Saúde Pública Universidade Federal de Goiás
dc.description.affiliationUniversidade Brasil – Campus Descalvado
dc.description.affiliationUnespFaculdade de Ciências Agrárias e Veterinárias UNESP/CPPAR, Access route Prof. Paulo Donatto Castellane, no number, Jaboticabal, São Paulo
dc.format.extent90-93
dc.identifierhttp://dx.doi.org/10.1016/j.vetpar.2017.04.003
dc.identifier.citationVeterinary Parasitology, v. 238, p. 90-93.
dc.identifier.doi10.1016/j.vetpar.2017.04.003
dc.identifier.file2-s2.0-85017397358.pdf
dc.identifier.issn1873-2550
dc.identifier.issn0304-4017
dc.identifier.lattes5950594366829647
dc.identifier.scopus2-s2.0-85017397358
dc.identifier.urihttp://hdl.handle.net/11449/174456
dc.language.isoeng
dc.relation.ispartofVeterinary Parasitology
dc.relation.ispartofsjr1,275
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectFelines
dc.subjectPCR
dc.subjectSexual transmission
dc.subjectTachyzoites
dc.subjectToxoplasmosis
dc.titleInvestigation of Toxoplasma gondii in semen, testicle and epididymis tissues of primo-infected cats (Felis catus)en
dc.typeArtigo
unesp.author.lattes5950594366829647
unesp.author.lattes4587241487071929[12]

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