In vitro evaluation of the antimicrobial activity of a castor oil-based irrigant

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dc.contributor.authorLeonardo, Mario Roberto [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:20:20Z
dc.date.available2014-05-27T11:20:20Z
dc.date.issued2001-12-01
dc.description.abstractThe antimicrobial activity of irrigating solutions - Endoquil (castor oil detergent), 2% chlorhexidine gluconate solution, and 0.5% NaOCI solution - was evaluated against Gram-positive cocci (Micrococcus luteus, Staphylococcus aureus, Enterococcus faecalis, Staphylococcus epidermidis, Streptococcus mutans, and Streptococcus sobrinus), Gramnegative rods (Escherichia coli and Pseudomonas aeruginosa), and the yeast Candida albicans. Activity was evaluated using the two-layer agar diffusion technique. The base layer was obtained by pouring 10.0 ml of Muller Hinton Medium or 10.0 ml of Brain Heart Infusion agar in a Petri dish. After solidification a 5.0 ml seed layer of Muller Hinton Medium or Brain Heart Infusion agar with inoculum (106/ml) was added. Absorbent paper disks (6.0 mm in diameter) immersed in the solutions were placed at equidistant points. Plates were maintained at room temperature for 2 h for prediffusion of the solutions and incubated at 37°C for 24 h. The candle jar system was used for the Brain Heart Infusion agar plates. All tests were performed in duplicate. After incubation the medium was optimized with 0.05 g% triphenyltetrazolium chlorate gel and inhibition halos were measured. All bacterial strains were inhibited by 2.0% chlorhexidine gluconate. Endoquil was effective against Grampositive microorganisms, and 0.5% NaOCI was effective only against S. aureus. Copyright © 2001 by The American Association of Endodontists.en
dc.description.affiliationDepartamento de Endodontia Faculdade de Odontologia de Araraquara UNESP, Rua Humaitá, 1680, 14801-903, Araraquara, SP
dc.description.affiliationUnespDepartamento de Endodontia Faculdade de Odontologia de Araraquara UNESP, Rua Humaitá, 1680, 14801-903, Araraquara, SP
dc.format.extent717-719
dc.identifierhttp://dx.doi.org/10.1097/00004770-200112000-00001
dc.identifier.citationJournal of Endodontics, v. 27, n. 12, p. 717-719, 2001.
dc.identifier.doi10.1097/00004770-200112000-00001
dc.identifier.issn0099-2399
dc.identifier.scopus2-s2.0-0035656306
dc.identifier.urihttp://hdl.handle.net/11449/66657
dc.identifier.wosWOS:000172573900001
dc.language.isoeng
dc.relation.ispartofJournal of Endodontics
dc.relation.ispartofjcr2.886
dc.relation.ispartofsjr1,585
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAnti Infective Agents, Local
dc.subjectbiomedical and dental materials
dc.subjectcastor oil
dc.subjectchlorhexidine
dc.subjectchlorhexidine gluconate
dc.subjectdrug derivative
dc.subjecthypochlorite sodium
dc.subjecttopical antiinfective agent
dc.subjectCandida albicans
dc.subjectcomparative study
dc.subjectdrug effect
dc.subjectGram negative bacterium
dc.subjectGram positive cocci
dc.subjectmicrobiological examination
dc.subjectAnti-Infective Agents, Local
dc.subjectCastor Oil
dc.subjectChlorhexidine
dc.subjectComparative Study
dc.subjectGram-Negative Bacteria
dc.subjectGram-Positive Cocci
dc.subjectMicrobial Sensitivity Tests
dc.subjectRoot Canal Irrigants
dc.subjectSodium Hypochlorite
dc.titleIn vitro evaluation of the antimicrobial activity of a castor oil-based irriganten
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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