Microdissection and Chromosome Painting

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2015-01-01

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Vicari, M. R.
Pansonato-Alves, J. C. [UNESP]
Foresti, F. [UNESP]

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The principle of chromosome microdissection technique is based on the identification of either a chromosome, chromosome arm or chromosome band for the subsequent removal of such target with the aid of a micromanipulator attached to an inverted microscope. Once the target is defined, whole chromosomes, or chromosomal regions, are directly isolated under an inverted microscope linked to a micromanipulator. Following dissection, chromosomal fragments pass through a step of amplification, using adapted methods of whole genome amplification (WGA) in order to increase the limited amount of DNA (Telenius et al. 1992; Zhang et al. 1992) and reduce the starting copies material (Fig. 1).

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Fish Cytogenetic Techniques: Ray-Fin Fishes and Chondrichthyans, p. 144-165.