Genotoxicity of the medicinal plant Maytenus robusta in mammalian cells in vivo.

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dc.contributor.authorRaymundo, T. M. [UNESP]
dc.contributor.authorFavilla, M. [UNESP]
dc.contributor.authorNiero, R.
dc.contributor.authorAndrade, S. F.
dc.contributor.authorMaistro, E. L. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:27:21Z
dc.date.available2014-05-27T11:27:21Z
dc.date.issued2012-12-01
dc.description.abstractPlants belonging to the Celastraceae family have been used in traditional medicine for their analgesic, anti-inflammatory and anti-ulcerogenic properties, among others. Maytenus ilicifolia is the principal species of this family, and is used in the treatment of gastric ulcers. However, owing to its inadequate management in Brazil, the species is becoming extinct and is being substituted with Maytenus robusta, which also displays gastroprotective activity. The aim of this study was to evaluate the genotoxic effects of M. robusta hydroalcoholic extract in vivo, using the comet assay and micronucleus test. Three doses (50, 250 and 500 mg/kg body weight) were administered to mice orally 2 times at 24-h intervals. Cytotoxicity was assessed by scoring 200 consecutive total polychromatic and normochromatic erythrocytes to calculate their ratio. Parametric (analysis of variance/Tukey) and non-parametric (Kruskal-Wallis/Dunn post hoc) tests were used to evaluate the results according to the nature of the data distribution. The results showed a significant increase in the frequency of DNA damage on leukocytes at the 2 higher doses tested, but the extract did not enhance micronucleus frequency in bone marrow cells. Our findings showed that after 48 h of treatment, M. robusta hydroalcoholic extract had weak genotoxic effects but no clastogenic effects in mice cells.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 08/51175-7
dc.format.extent2847-2854
dc.identifierhttp://dx.doi.org/10.4238/2012.August.24.9
dc.identifier.citationGenetics and molecular research : GMR, v. 11, n. 3, p. 2847-2854, 2012.
dc.identifier.doi10.4238/2012.August.24.9
dc.identifier.file2-s2.0-84872017335.pdf
dc.identifier.issn1676-5680
dc.identifier.scopus2-s2.0-84872017335
dc.identifier.urihttp://hdl.handle.net/11449/73902
dc.identifier.wosWOS:000308817800102
dc.language.isoeng
dc.relation.ispartofGenetics and molecular research : GMR
dc.relation.ispartofsjr0,439
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectDNA
dc.subjectmutagenic agent
dc.subjectplant extract
dc.subjectanimal
dc.subjectbone marrow cell
dc.subjectchemistry
dc.subjectcomet assay
dc.subjectdrug effect
dc.subjecterythrocyte
dc.subjectleukocyte
dc.subjectmale
dc.subjectmammal
dc.subjectMaytenus
dc.subjectmedicinal plant
dc.subjectmetabolism
dc.subjectmicronucleus
dc.subjectmouse
dc.subjectmutagen testing
dc.subjectAnimals
dc.subjectBone Marrow Cells
dc.subjectComet Assay
dc.subjectErythrocytes
dc.subjectLeukocytes
dc.subjectMale
dc.subjectMammals
dc.subjectMice
dc.subjectMicronuclei, Chromosome-Defective
dc.subjectMutagenicity Tests
dc.subjectMutagens
dc.subjectPlant Extracts
dc.subjectPlants, Medicinal
dc.titleGenotoxicity of the medicinal plant Maytenus robusta in mammalian cells in vivo.en
dc.typeArtigo
dcterms.licensehttp://www.geneticsmr.com/node/5
unesp.author.orcid0000-0003-0757-7876[5]

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