Citotoxicity of Fipronil on Hepatocytes Isolated from Rat and Effects of Its Biotransformation

Página do item simplificado
dc.contributor.authorGuelfi, Marieli [UNESP]
dc.contributor.authorMaioli, Marcos Antonio [UNESP]
dc.contributor.authorTavares, Marco Aurelio [UNESP]
dc.contributor.authorMingatto, Fabio Erminio [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-11-26T16:18:53Z
dc.date.available2018-11-26T16:18:53Z
dc.date.issued2015-11-01
dc.description.abstractThe aim of this study was to characterize the mechanism of toxicity of fipronil on hepatocytes isolated from the rat and the effect of its biotransformation on the toxicological potential. The toxicity of fipronil was assessed by monitoring the oxygen consumption and mitochondrial membrane potential, intracellular ATP concentration, Ca2+ homeostasis and cell viability. The cell viability was evaluated by trypan blue exclusion in hepatocytes that were isolated from the normal rats and by the release of the enzymes alanine transaminase and aspartate transaminase in hepatocytes that were isolated from the normal rats or proadifen-pretreated rats. Fipronil reduced mitochondrial respiration in the cells that were energized with glutamate plus malate in a dose-dependent manner and dissipated the mitochondrial membrane potential that was accompanied by a reduction in ATP concentration and a disruption of intracellular Ca2+ homeostasis. The cell viability was affected by fipronil with higher potency in hepatocytes that were isolated from the normal rats, which indicated that the metabolism of this insecticide increased its toxicological potential. The results of this study indicated that the toxicity of fipronil to the hepatocytes was related to the inhibition of mitochondrial activity, which led to decreased ATP synthesis and a consequent alteration in intracellular Ca2+ homeostasis and ultimately resulted in cell death.en
dc.description.affiliationUniv Estadual Paulista, Dept Zootecnia, Dracena, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Prod & Saude Anim, Aracatuba, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Zootecnia, Dracena, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Prod & Saude Anim, Aracatuba, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2012/15135-6
dc.description.sponsorshipIdFAPESP: 2012/24872-4
dc.format.extent843-853
dc.identifierhttp://dx.doi.org/10.1590/S1516-89132015060298
dc.identifier.citationBrazilian Archives Of Biology And Technology. Curitiba-parana: Inst Tecnologia Parana, v. 58, n. 6, p. 843-853, 2015.
dc.identifier.doi10.1590/S1516-89132015060298
dc.identifier.fileS1516-89132015000600843.pdf
dc.identifier.issn1516-8913
dc.identifier.scieloS1516-89132015000600843
dc.identifier.urihttp://hdl.handle.net/11449/161033
dc.identifier.wosWOS:000366277400004
dc.language.isoeng
dc.publisherInst Tecnologia Parana
dc.relation.ispartofBrazilian Archives Of Biology And Technology
dc.relation.ispartofsjr0,281
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectfipronil
dc.subjecthepatotoxicity
dc.subjectbioenergetics
dc.subjectbiotransformation
dc.subjectcalcium homeostasis
dc.titleCitotoxicity of Fipronil on Hepatocytes Isolated from Rat and Effects of Its Biotransformationen
dc.typeArtigo
dcterms.rightsHolderInst Tecnologia Parana
unesp.author.orcid0000-0003-3488-1814[4]
unesp.departmentZootecnia - FCATpt

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
S1516-89132015000600843.pdf
Tamanho:
198.22 KB
Formato:
Adobe Portable Document Format
Baixar

Coleções

Dracena - FCAT - Faculdade de Ciências Agrárias e Tecnológicas
Artigos - Apoio, Produção e Saúde Animal - FMVA