Cyclodextrin glycosyltransferase from Bacillus licheniformis: Optimization of production and its properties

dc.contributor.authorMartins Bonilha, Paulo Roberto
dc.contributor.authorMenocci, Vivian
dc.contributor.authorGoulart, Antonio Jose
dc.contributor.authorTeixeira de Moraes Polizeli, Maria de Lourdes
dc.contributor.authorMonti, Rubens [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:24:25Z
dc.date.available2014-05-20T13:24:25Z
dc.date.issued2006-07-01
dc.description.abstractCyclodextrin glycosyltransferase (EC 2.4.1.19) is an enzyme that produces cyclodextrins from starch via an intramolecular transglycosylation reaction. An alkalophilic Bacillus strain, isolated from cassava peels, was identified as Bacillus licheniformis. CGTase production by this strain was better when potato starch was used as carbon source, followed by cassava starch and amylopectin. Glucose and amylose, on the other hand, acted as synthesis repressors. When the cultivation was supplemented with sodium ions and had the pH adjusted between 6.0 and 9.0, the microorganism maintained the growth and enzyme production capacity. This data is interesting because it contradicts the concept that alkalophilic microorganisms do not grow in this pH range. After ultrafiltration-centrifugation, one protein of 85.2 kDa with CGTase activity was isolated. This protein was identified in plates with starch and phenolphthalein. Determination of the optimum temperature showed higher activities at 25 degrees C and 55 degrees C, indicating the possible presence of more than one CGTase in the culture filtrate. Km and Vmax values were 1.77 mg/mL and 0.0263 U/mg protein, respectively, using potato starch as substrate.en
dc.description.affiliationUniv Estadual Paulista, Inst Quim, Dept Bioquim & Quim Tecnol, Araraquara, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Ciências Farmaceut, Dept Alimentos & Nutr, Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Quim, Dept Bioquim & Quim Tecnol, Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciências Farmaceut, Dept Alimentos & Nutr, Araraquara, SP, Brazil
dc.format.extent317-323
dc.identifierhttp://dx.doi.org/10.1590/S1517-83822006000300022
dc.identifier.citationBrazilian Journal of Microbiology. São Paulo: Soc Brasileira Microbiologia, v. 37, n. 3, p. 317-323, 2006.
dc.identifier.doi10.1590/S1517-83822006000300022
dc.identifier.fileWOS000243335200022.pdf
dc.identifier.issn1517-8382
dc.identifier.scieloS1517-83822006000300022
dc.identifier.urihttp://hdl.handle.net/11449/7567
dc.identifier.wosWOS:000243335200022
dc.language.isoeng
dc.publisherSoc Brasileira Microbiologia
dc.relation.ispartofBrazilian Journal of Microbiology
dc.relation.ispartofjcr1.810
dc.relation.ispartofsjr0,630
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectcyclodextrin glycosyltransferasept
dc.subjectCGTase productionpt
dc.subjectBacillus licheniformispt
dc.subjectBacillus beta-CDpt
dc.titleCyclodextrin glycosyltransferase from Bacillus licheniformis: Optimization of production and its propertiesen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/bjm/iaboutj.htm
dcterms.rightsHolderSoc Brasileira Microbiologia
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Carregando...
Imagem de Miniatura
Nome:
WOS000243335200022.pdf
Tamanho:
865.89 KB
Formato:
Adobe Portable Document Format
Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição: