Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish Model
dc.contributor.author | Rosa, Ivana F. [UNESP] | |
dc.contributor.author | Peçanha, Ana P. B. | |
dc.contributor.author | Carvalho, Tábata R. B. | |
dc.contributor.author | Alexandre, Leonardo S. | |
dc.contributor.author | Ferreira, Vinícius G. | |
dc.contributor.author | Doretto, Lucas B. [UNESP] | |
dc.contributor.author | Souza, Beatriz M. [UNESP] | |
dc.contributor.author | Nakajima, Rafael T. [UNESP] | |
dc.contributor.author | da Silva, Patrick | |
dc.contributor.author | Barbosa, Ana P. | |
dc.contributor.author | Gomes-de-Pontes, Leticia | |
dc.contributor.author | Bomfim, Camila G. | |
dc.contributor.author | Machado-Santelli, Glaucia M. | |
dc.contributor.author | Condino-Neto, Antonio | |
dc.contributor.author | Guzzo, Cristiane R. | |
dc.contributor.author | Peron, Jean P. S. | |
dc.contributor.author | Andrade-Silva, Magaiver | |
dc.contributor.author | Câmara, Niels O. S. | |
dc.contributor.author | Garnique, Anali M. B. | |
dc.contributor.author | Medeiros, Renata J. | |
dc.contributor.author | Ferraris, Fausto K. | |
dc.contributor.author | Barcellos, Leonardo J. G. | |
dc.contributor.author | Correia-Junior, Jose D. | |
dc.contributor.author | Galindo-Villegas, Jorge | |
dc.contributor.author | Machado, Mônica F. R. | |
dc.contributor.author | Castoldi, Angela | |
dc.contributor.author | Oliveira, Susana L. [UNESP] | |
dc.contributor.author | Costa, Camila C. [UNESP] | |
dc.contributor.author | Belo, Marco A. A. [UNESP] | |
dc.contributor.author | Galdino, Giovane | |
dc.contributor.author | Sgro, Germán G. | |
dc.contributor.author | Bueno, Natalia F. | |
dc.contributor.author | Eto, Silas F. | |
dc.contributor.author | Veras, Flávio P. | |
dc.contributor.author | Fernandes, Bianca H. V. | |
dc.contributor.author | Sanches, Paulo R. S. [UNESP] | |
dc.contributor.author | Cilli, Eduardo M. [UNESP] | |
dc.contributor.author | Malafaia, Guilherme | |
dc.contributor.author | Nóbrega, Rafael H. [UNESP] | |
dc.contributor.author | Garcez, Aguinaldo S. | |
dc.contributor.author | Carrilho, Emanuel | |
dc.contributor.author | Charlie-Silva, Ives [UNESP] | |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | |
dc.contributor.institution | São Leopoldo Mandic College | |
dc.contributor.institution | Universidade de São Paulo (USP) | |
dc.contributor.institution | INCTBio | |
dc.contributor.institution | Oswaldo Cruz Foundation (Fiocruz) | |
dc.contributor.institution | Universidade de Passo Fundo | |
dc.contributor.institution | Federal University Minas Gerais | |
dc.contributor.institution | Nord University | |
dc.contributor.institution | Federal University of Jatai | |
dc.contributor.institution | Universidade Federal de Pernambuco (UFPE) | |
dc.contributor.institution | Federal University of Alfenas | |
dc.contributor.institution | FIOCRUZ Paraná | |
dc.contributor.institution | Laboratory of Development and Innovation Butantan Institute | |
dc.contributor.institution | Goiano Federal Institute | |
dc.date.accessioned | 2023-07-29T13:51:36Z | |
dc.date.available | 2023-07-29T13:51:36Z | |
dc.date.issued | 2023-04-01 | |
dc.description.abstract | Although the exact mechanism of the pathogenesis of coronavirus SARS-CoV-2 (COVID-19) is not fully understood, oxidative stress and the release of pro-inflammatory cytokines have been highlighted as playing a vital role in the pathogenesis of the disease. In this sense, alternative treatments are needed to reduce the level of inflammation caused by COVID-19. Therefore, this study aimed to investigate the potential effect of red photobiomodulation (PBM) as an attractive therapy to downregulate the cytokine storm caused by COVID-19 in a zebrafish model. RT-qPCR analyses and protein–protein interaction prediction among SARS-CoV-2 and Danio rerio proteins showed that recombinant Spike protein (rSpike) was responsible for generating systemic inflammatory processes with significantly increased levels of pro-inflammatory (il1b, il6, tnfa, and nfkbiab), oxidative stress (romo1) and energy metabolism (slc2a1a and coa1) mRNA markers, with a pattern similar to those observed in COVID-19 cases in humans. On the other hand, PBM treatment was able to decrease the mRNA levels of these pro-inflammatory and oxidative stress markers compared with rSpike in various tissues, promoting an anti-inflammatory response. Conversely, PBM promotes cellular and tissue repair of injured tissues and significantly increases the survival rate of rSpike-inoculated individuals. Additionally, metabolomics analysis showed that the most-impacted metabolic pathways between PBM and the rSpike treated groups were related to steroid metabolism, immune system, and lipid metabolism. Together, our findings suggest that the inflammatory process is an incisive feature of COVID-19 and red PBM can be used as a novel therapeutic agent for COVID-19 by regulating the inflammatory response. Nevertheless, the need for more clinical trials remains, and there is a significant gap to overcome before clinical trials can commence. | en |
dc.description.affiliation | Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP) | |
dc.description.affiliation | Department of Orthodontics São Leopoldo Mandic College | |
dc.description.affiliation | Instituto de Química de São Carlos Universidade de São Paulo | |
dc.description.affiliation | The National Institute of Science and Technology in Bioanalyses INCTBio | |
dc.description.affiliation | Institute of Biomedical Sciences University of São Paulo (USP) | |
dc.description.affiliation | Oswaldo Cruz Foundation (Fiocruz) | |
dc.description.affiliation | Laboratório de Fisiologia de Peixes Programa de Pós-Graduação em Bioexperimentação Escola de Ciências Agrárias Inovação e Negócios Universidade de Passo Fundo | |
dc.description.affiliation | Institute of Biomedical Sciences Federal University Minas Gerais | |
dc.description.affiliation | Department of Genomics Faculty of Biosciences and Aquaculture Nord University | |
dc.description.affiliation | Biological Sciences Special Academic Unit Federal University of Jatai | |
dc.description.affiliation | Keizo Asami Institute Federal University of Pernambuco | |
dc.description.affiliation | School of Agricultural and Veterinary Sciences São Paulo State University (UNESP) | |
dc.description.affiliation | Institute of Motricity Sciences Department of Physical Therapy Federal University of Alfenas | |
dc.description.affiliation | Departamento de Ciências Biomoleculares Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo | |
dc.description.affiliation | Integrated Structural Biology Platform Carlos Chagas Institute FIOCRUZ Paraná | |
dc.description.affiliation | Center of Innovation and Development Laboratory of Development and Innovation Butantan Institute | |
dc.description.affiliation | Faculty of Medicine University of São Paulo (USP) | |
dc.description.affiliation | Laboratory of Genetic and Sanitary Control Technical Board of Support for Teaching and Research Faculty of Medicine University of Sao Paulo | |
dc.description.affiliation | Department of Biochemistry and Organic Chemistry Institute of Chemistry São Paulo State University (UNESP) | |
dc.description.affiliation | Laboratory of Toxicology Applied to the Environment Goiano Federal Institute, Urutaí Campus | |
dc.description.affiliationUnesp | Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP) | |
dc.description.affiliationUnesp | School of Agricultural and Veterinary Sciences São Paulo State University (UNESP) | |
dc.description.affiliationUnesp | Department of Biochemistry and Organic Chemistry Institute of Chemistry São Paulo State University (UNESP) | |
dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
dc.description.sponsorshipId | CAPES: 0459/20 | |
dc.description.sponsorshipId | CAPES: 110 | |
dc.description.sponsorshipId | CAPES: 465389/2014-7 | |
dc.description.sponsorshipId | CAPES: 88887.504531/2020-00 | |
dc.description.sponsorshipId | CAPES: Project CG 19 | |
dc.identifier | http://dx.doi.org/10.3390/ijms24076104 | |
dc.identifier.citation | International Journal of Molecular Sciences, v. 24, n. 7, 2023. | |
dc.identifier.doi | 10.3390/ijms24076104 | |
dc.identifier.issn | 1422-0067 | |
dc.identifier.issn | 1661-6596 | |
dc.identifier.scopus | 2-s2.0-85152799400 | |
dc.identifier.uri | http://hdl.handle.net/11449/248713 | |
dc.language.iso | eng | |
dc.relation.ispartof | International Journal of Molecular Sciences | |
dc.source | Scopus | |
dc.subject | COVID-19 | |
dc.subject | cytokine storms | |
dc.subject | oxidative stress | |
dc.subject | photobiomodulation | |
dc.subject | rSpike | |
dc.subject | zebrafish | |
dc.title | Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish Model | en |
dc.type | Artigo | |
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