Photobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish Model

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dc.contributor.authorRosa, Ivana F. [UNESP]
dc.contributor.authorPeçanha, Ana P. B.
dc.contributor.authorCarvalho, Tábata R. B.
dc.contributor.authorAlexandre, Leonardo S.
dc.contributor.authorFerreira, Vinícius G.
dc.contributor.authorDoretto, Lucas B. [UNESP]
dc.contributor.authorSouza, Beatriz M. [UNESP]
dc.contributor.authorNakajima, Rafael T. [UNESP]
dc.contributor.authorda Silva, Patrick
dc.contributor.authorBarbosa, Ana P.
dc.contributor.authorGomes-de-Pontes, Leticia
dc.contributor.authorBomfim, Camila G.
dc.contributor.authorMachado-Santelli, Glaucia M.
dc.contributor.authorCondino-Neto, Antonio
dc.contributor.authorGuzzo, Cristiane R.
dc.contributor.authorPeron, Jean P. S.
dc.contributor.authorAndrade-Silva, Magaiver
dc.contributor.authorCâmara, Niels O. S.
dc.contributor.authorGarnique, Anali M. B.
dc.contributor.authorMedeiros, Renata J.
dc.contributor.authorFerraris, Fausto K.
dc.contributor.authorBarcellos, Leonardo J. G.
dc.contributor.authorCorreia-Junior, Jose D.
dc.contributor.authorGalindo-Villegas, Jorge
dc.contributor.authorMachado, Mônica F. R.
dc.contributor.authorCastoldi, Angela
dc.contributor.authorOliveira, Susana L. [UNESP]
dc.contributor.authorCosta, Camila C. [UNESP]
dc.contributor.authorBelo, Marco A. A. [UNESP]
dc.contributor.authorGaldino, Giovane
dc.contributor.authorSgro, Germán G.
dc.contributor.authorBueno, Natalia F.
dc.contributor.authorEto, Silas F.
dc.contributor.authorVeras, Flávio P.
dc.contributor.authorFernandes, Bianca H. V.
dc.contributor.authorSanches, Paulo R. S. [UNESP]
dc.contributor.authorCilli, Eduardo M. [UNESP]
dc.contributor.authorMalafaia, Guilherme
dc.contributor.authorNóbrega, Rafael H. [UNESP]
dc.contributor.authorGarcez, Aguinaldo S.
dc.contributor.authorCarrilho, Emanuel
dc.contributor.authorCharlie-Silva, Ives [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionSão Leopoldo Mandic College
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionINCTBio
dc.contributor.institutionOswaldo Cruz Foundation (Fiocruz)
dc.contributor.institutionUniversidade de Passo Fundo
dc.contributor.institutionFederal University Minas Gerais
dc.contributor.institutionNord University
dc.contributor.institutionFederal University of Jatai
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.contributor.institutionFederal University of Alfenas
dc.contributor.institutionFIOCRUZ Paraná
dc.contributor.institutionLaboratory of Development and Innovation Butantan Institute
dc.contributor.institutionGoiano Federal Institute
dc.date.accessioned2023-07-29T13:51:36Z
dc.date.available2023-07-29T13:51:36Z
dc.date.issued2023-04-01
dc.description.abstractAlthough the exact mechanism of the pathogenesis of coronavirus SARS-CoV-2 (COVID-19) is not fully understood, oxidative stress and the release of pro-inflammatory cytokines have been highlighted as playing a vital role in the pathogenesis of the disease. In this sense, alternative treatments are needed to reduce the level of inflammation caused by COVID-19. Therefore, this study aimed to investigate the potential effect of red photobiomodulation (PBM) as an attractive therapy to downregulate the cytokine storm caused by COVID-19 in a zebrafish model. RT-qPCR analyses and protein–protein interaction prediction among SARS-CoV-2 and Danio rerio proteins showed that recombinant Spike protein (rSpike) was responsible for generating systemic inflammatory processes with significantly increased levels of pro-inflammatory (il1b, il6, tnfa, and nfkbiab), oxidative stress (romo1) and energy metabolism (slc2a1a and coa1) mRNA markers, with a pattern similar to those observed in COVID-19 cases in humans. On the other hand, PBM treatment was able to decrease the mRNA levels of these pro-inflammatory and oxidative stress markers compared with rSpike in various tissues, promoting an anti-inflammatory response. Conversely, PBM promotes cellular and tissue repair of injured tissues and significantly increases the survival rate of rSpike-inoculated individuals. Additionally, metabolomics analysis showed that the most-impacted metabolic pathways between PBM and the rSpike treated groups were related to steroid metabolism, immune system, and lipid metabolism. Together, our findings suggest that the inflammatory process is an incisive feature of COVID-19 and red PBM can be used as a novel therapeutic agent for COVID-19 by regulating the inflammatory response. Nevertheless, the need for more clinical trials remains, and there is a significant gap to overcome before clinical trials can commence.en
dc.description.affiliationDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP)
dc.description.affiliationDepartment of Orthodontics São Leopoldo Mandic College
dc.description.affiliationInstituto de Química de São Carlos Universidade de São Paulo
dc.description.affiliationThe National Institute of Science and Technology in Bioanalyses INCTBio
dc.description.affiliationInstitute of Biomedical Sciences University of São Paulo (USP)
dc.description.affiliationOswaldo Cruz Foundation (Fiocruz)
dc.description.affiliationLaboratório de Fisiologia de Peixes Programa de Pós-Graduação em Bioexperimentação Escola de Ciências Agrárias Inovação e Negócios Universidade de Passo Fundo
dc.description.affiliationInstitute of Biomedical Sciences Federal University Minas Gerais
dc.description.affiliationDepartment of Genomics Faculty of Biosciences and Aquaculture Nord University
dc.description.affiliationBiological Sciences Special Academic Unit Federal University of Jatai
dc.description.affiliationKeizo Asami Institute Federal University of Pernambuco
dc.description.affiliationSchool of Agricultural and Veterinary Sciences São Paulo State University (UNESP)
dc.description.affiliationInstitute of Motricity Sciences Department of Physical Therapy Federal University of Alfenas
dc.description.affiliationDepartamento de Ciências Biomoleculares Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo
dc.description.affiliationIntegrated Structural Biology Platform Carlos Chagas Institute FIOCRUZ Paraná
dc.description.affiliationCenter of Innovation and Development Laboratory of Development and Innovation Butantan Institute
dc.description.affiliationFaculty of Medicine University of São Paulo (USP)
dc.description.affiliationLaboratory of Genetic and Sanitary Control Technical Board of Support for Teaching and Research Faculty of Medicine University of Sao Paulo
dc.description.affiliationDepartment of Biochemistry and Organic Chemistry Institute of Chemistry São Paulo State University (UNESP)
dc.description.affiliationLaboratory of Toxicology Applied to the Environment Goiano Federal Institute, Urutaí Campus
dc.description.affiliationUnespDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP)
dc.description.affiliationUnespSchool of Agricultural and Veterinary Sciences São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Biochemistry and Organic Chemistry Institute of Chemistry São Paulo State University (UNESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdCAPES: 0459/20
dc.description.sponsorshipIdCAPES: 110
dc.description.sponsorshipIdCAPES: 465389/2014-7
dc.description.sponsorshipIdCAPES: 88887.504531/2020-00
dc.description.sponsorshipIdCAPES: Project CG 19
dc.identifierhttp://dx.doi.org/10.3390/ijms24076104
dc.identifier.citationInternational Journal of Molecular Sciences, v. 24, n. 7, 2023.
dc.identifier.doi10.3390/ijms24076104
dc.identifier.issn1422-0067
dc.identifier.issn1661-6596
dc.identifier.scopus2-s2.0-85152799400
dc.identifier.urihttp://hdl.handle.net/11449/248713
dc.language.isoeng
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.sourceScopus
dc.subjectCOVID-19
dc.subjectcytokine storms
dc.subjectoxidative stress
dc.subjectphotobiomodulation
dc.subjectrSpike
dc.subjectzebrafish
dc.titlePhotobiomodulation Reduces the Cytokine Storm Syndrome Associated with COVID-19 in the Zebrafish Modelen
dc.typeArtigo
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unesp.author.orcid0000-0001-7351-8220[41]

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