Bioconversion of crude glycerol from waste cooking oils into hydrogen by sub-tropical mixed and pure cultures

dc.contributor.authorRodrigues, Caroline Varella [UNESP]
dc.contributor.authorNespeca, Maurílio Gustavo [UNESP]
dc.contributor.authorSakamoto, Isabel Kimiko
dc.contributor.authorde Oliveira, José Eduardo [UNESP]
dc.contributor.authorAmâncio Varesche, Maria Bernadete
dc.contributor.authorMaintinguer, Sandra Imaculada [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversity of Araraquara
dc.date.accessioned2018-12-11T17:36:23Z
dc.date.available2018-12-11T17:36:23Z
dc.date.issued2018-01-01
dc.description.abstractThis study compared the biohydrogen generation by sub-tropical mixed and pure cultures from the crude glycerol from the biodiesel production using waste cooking oils (WCO). The crude glycerol was pretreated by pH adjustment. The mixed culture was obtained from a subtropical granular sludge of the UASB (Upflow Anaerobic Sludge Blanket) reactor used in the treatment of vinasse from sugarcane of ethanol and sugar industry. It was heat treated in order to inactivate hydrogen-consuming bacteria, which was identified by Illumina MiSeq Sequencing with a relative abundance of 97.96% Firmicutes Philum, 91.81% Clostridia Class and 91.81% Clostridiales Order. The pure culture was isolated from a sub-tropical granular sludge from UASB reactor of treating brewery wastewater and identified as Enterobacter sp. (KP893397). Two assays were carried in anaerobic batch reactors in order to verify the hydrogen production from crude glycerol bioconversion with: (I) mixed culture and (II) pure culture. The experiments were conducted at 37 °C, initial pH of 5.5 for assay I and 7.0 for assay II, with 20 g COD L−1 of crude glycerol. The crude glycerol consumption was 56.2% and 88.0% for the assay I and II, respectively. The hydrogen yields were 0.80 moL H2 mol−1 glycerol for the assay I and 0.13 moL H2 mol−1 glycerol for the assay II. Enterobacter sp. preferred the reductive metabolic route, generating 1460.0 mg L−1 of 1,3-propanediol, and it showed to be more sensitive in the presence of methanol from crude glycerol than mixed culture that preferred the oxidative metabolic route with biohydrogen generation. The mixed culture was more able to generate H2 than pure culture from the crude glycerol coming from the biodiesel production using WCO.en
dc.description.affiliationInstitute of Chemistry-IQ UNESP, Prof. Francisco Degni 55, Zip Code
dc.description.affiliationBioenergy Research Institute-IPBEN UNESP
dc.description.affiliationCenter for Monitoring and Research of the Quality of Fuels Biofuels Crude Oil and Derivatives Institute of Chemistry-CEMPEQC UNESP, Prof. Francisco Degni 55, Zip Code
dc.description.affiliationDepartment of Hydraulics and Sanitation School of Engineering of São Carlos University of São Paulo Av. João Dagnone, 1100, Jd. Santa Angelina, Zip Code
dc.description.affiliationUniversity of Araraquara, R. Voluntários da Pátria, 1309, Zip Code
dc.description.affiliationUnespInstitute of Chemistry-IQ UNESP, Prof. Francisco Degni 55, Zip Code
dc.description.affiliationUnespBioenergy Research Institute-IPBEN UNESP
dc.description.affiliationUnespCenter for Monitoring and Research of the Quality of Fuels Biofuels Crude Oil and Derivatives Institute of Chemistry-CEMPEQC UNESP, Prof. Francisco Degni 55, Zip Code
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCAPES: 141038/2017-9
dc.description.sponsorshipIdFAPESP: 2017/11767-1
dc.description.sponsorshipIdCNPq: CNPq-Proc 457144/2014-9
dc.description.sponsorshipIdFAPESP: FAPESP Processes 2012/01318-01
dc.identifierhttp://dx.doi.org/10.1016/j.ijhydene.2018.02.174
dc.identifier.citationInternational Journal of Hydrogen Energy.
dc.identifier.doi10.1016/j.ijhydene.2018.02.174
dc.identifier.file2-s2.0-85044330357.pdf
dc.identifier.issn0360-3199
dc.identifier.lattes2967035823175406
dc.identifier.orcid0000-0002-4584-7649
dc.identifier.scopus2-s2.0-85044330357
dc.identifier.urihttp://hdl.handle.net/11449/179695
dc.language.isoeng
dc.relation.ispartofInternational Journal of Hydrogen Energy
dc.relation.ispartofsjr1,116
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subject1,3-Propanediol
dc.subjectBiodiesel
dc.subjectBiohydrogen
dc.subjectClostridium sp.
dc.subjectEnterobacter sp.
dc.subjectIllumina MiSeq sequencing
dc.titleBioconversion of crude glycerol from waste cooking oils into hydrogen by sub-tropical mixed and pure culturesen
dc.typeArtigo
unesp.author.lattes2967035823175406[6]
unesp.author.orcid0000-0002-9332-7023[2]
unesp.author.orcid0000-0003-1208-3483 0000-0003-1208-3483
unesp.author.orcid0000-0002-4584-7649[6]

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