Use of protein kinase C and phospholipase A2 inhibitors in bovine endometrial cells treated with estradiol and calcium ionophore

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dc.contributor.authorMaldonado, Mariângela Bueno Cordeiro [UNESP]
dc.contributor.authorHenry, Francine Messias Ciríaco
dc.contributor.authorFerreira, Teissiane Fernanda de Vasconcelos [UNESP]
dc.contributor.authorMello, Barbara Piffero
dc.contributor.authorBinelli, Mario
dc.contributor.authorMembrive, Claudia Maria Bertan [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionTexas Tech University
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversity of Florida
dc.date.accessioned2023-07-29T14:00:40Z
dc.date.available2023-07-29T14:00:40Z
dc.date.issued2021-03-23
dc.description.abstractThe release of endometrial prostaglandin-F2α (PGF2α) in bovine females can be induced in vivo by estradiol (E2). However, its role in this mechanism has not been clarified. We hypothesized that E2 stimulates the activity and abundance of protein kinase C (PKC) and phospholipase A2 (PLA2). Our objective in this study was to analyze the effects of PKC and PLA2 inhibitors on PGF2α synthesis induced by E2 and calcium ionophore (CI) in bovine endometrial cells (BEND cells; Experiment 1). Additionally, we evaluated the abundance of PKC and PLA2 in endometrial explants of cows treated or not with E2 17 days after estrus (D17, D0 = estrus; Experiment 2). In Experiment 1, BEND cells were submitted to a PKC inhibitor (10 µM of C25 H24 N4 O2; bisindolylmaleimide I, or BIS I), a PLA2 inhibitor (20 µM of arachydoniltrifluoromethane or AACOCF3), or none. The BEND cells were subsequently treated with E2 and CI, and PGF2α concentrations were measured in the culture medium through radioimmunoassay. For DIF-12 (PGF2α concentration 12 h after treatment subtracted from PGF2α concentration at hour 0), no PKC inhibitor effect was observed (P= 0.2709). However, DIF-12 was lower (P < 0.05) for groups treated with the PLA2 inhibitor and PLA2 inhibitor + CI + E2 groups than the control and CI + E2 groups. Thus, AACOCF3 was an efficient PLA2 inhibitor in the BEND cells culture system, and E2 did not stimulate the synthesis of PKC and PLA2. In Experiment 2, cyclic Nellore heifers received none (n = 5) or 3 mg (n = 6) of 17β-E2 on D17 and were slaughtered 2 h after administration. The abundance of PKC and PLA2 in the endometrial tissue was evaluated using Western blotting analysis. No E2 effect was observed on PKC (P = 0.08) and PLA2 (P = 0.56). We concluded that E2 did not stimulate the activity and abundance of PKC and PLA2.en
dc.description.affiliationUniversidade Estadual Paulista Faculdade de Ciências e Letras, SP
dc.description.affiliationTexas Tech University Department of Animal and Food Sciences
dc.description.affiliationUniversidade Estadual Paulista Faculdade de Ciências Agrárias e Tecnológicas Departamento de Produção Animal, SP
dc.description.affiliationUniversidade de São Paulo Faculdade de Medicina Veterinária e Zootecnia Departamento de Reprodução Animal, SP
dc.description.affiliationUniversity of Florida Department of Animal Sciences
dc.description.affiliationUnespUniversidade Estadual Paulista Faculdade de Ciências e Letras, SP
dc.description.affiliationUnespUniversidade Estadual Paulista Faculdade de Ciências Agrárias e Tecnológicas Departamento de Produção Animal, SP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2009/07482-5
dc.description.sponsorshipIdFAPESP: 2009/09234-9
dc.description.sponsorshipIdFAPESP: 2015/03331-3
dc.description.sponsorshipIdCNPq: 482786/2007-8
dc.identifierhttp://dx.doi.org/10.11606/ISSN.1678-4456.BJVRAS.2021.174355
dc.identifier.citationBrazilian Journal of Veterinary Research and Animal Science, v. 58.
dc.identifier.doi10.11606/ISSN.1678-4456.BJVRAS.2021.174355
dc.identifier.issn1678-4456
dc.identifier.issn1413-9596
dc.identifier.scopus2-s2.0-85147684612
dc.identifier.urihttp://hdl.handle.net/11449/249036
dc.language.isoeng
dc.relation.ispartofBrazilian Journal of Veterinary Research and Animal Science
dc.sourceScopus
dc.subjectBEND cells
dc.subjectEstradiol
dc.subjectPGF2α
dc.subjectPKC
dc.subjectPLA2
dc.titleUse of protein kinase C and phospholipase A2 inhibitors in bovine endometrial cells treated with estradiol and calcium ionophoreen
dc.titleUso de inibidores de proteína quinase C e fosfolipase A2 em células endometriais bovinas tratadas com estradiol e ionóforo de cálciopt
dc.typeArtigo

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