Densitometry, radiography, and histological assessment of collagen as methods to evaluate femoral bones in an experimental model of osteoporosis

dc.contributor.authorHernandes, L.
dc.contributor.authorRamos, A. L.
dc.contributor.authorMicheletti, K. R.
dc.contributor.authorSanti, A. P.
dc.contributor.authorCuoghi, O. A. [UNESP]
dc.contributor.authorSalazar, M.
dc.contributor.institutionUniversidade Estadual de Maringá (UEM)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:30:35Z
dc.date.available2014-05-20T15:30:35Z
dc.date.issued2012-02-01
dc.description.abstractAll methods to detect experimental loss of bone present technique limitations. The sensitivities of image and histological analyses to detect the effects of teriparatide in rats with bone loss after ovariectomy were evaluated. All methods were qualitatively valid.The standardization of methods to assess bone loss after ovariectomy is crucial to establish the degree of experimental osteoporosis. In general, methods per image or histological techniques are used. To validate these two ways to determine the degree of bone loss in ovariectomized rats, we evaluated the sensitivities of bone densitometry, conventional radiography, and histological analysis of the area occupied by collagen, detecting the effects of teriparatide treatment in the femur of ovariectomized rats with bone loss.Wistar rats were divided into three groups: a control group, in which the animals were only subjected to laparotomy; an ovariectomized group, in which bilateral removal of the ovaries was performed; and an ovariectomized + teriparatide group, in which bilateral removal of the ovaries was performed, and the animals were treated with 3 mu g/100 g/day of teriparatide. Three months following the ovariectomy, bone densitometry, radiographic densitometry, and histological analysis of the area occupied by collagen fibers were carried out in the femur diaphysis.The bone densitometry revealed 11.2% reduction in femur density; in the conventional radiography, the loss of bone mass was 14.5%, and with the histological analysis, a 40.9% reduction in the area occupied by collagen was detected in the femur diaphysis.In conclusion, histological analysis could not be quantitatively compared with the methods of bone densitometry and conventional radiography; however, all of these methods were qualitatively valid for assessing the loss of bone stemming from ovariectomy and the therapeutic effect of teriparatide in the prevention of osteoporosis.en
dc.description.affiliationUniversidade Estadual de Maringá (UEM), Dept Ciencias Morfol, BR-87020900 Maringa, Parana, Brazil
dc.description.affiliationUniversidade Estadual de Maringá (UEM), Odontol Dept, BR-87080000 Maringa, Parana, Brazil
dc.description.affiliationUniv Estadual Paulista, Odontol Dept, BR-16015050 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Odontol Dept, BR-16015050 São Paulo, Brazil
dc.format.extent467-473
dc.identifierhttp://dx.doi.org/10.1007/s00198-011-1539-8
dc.identifier.citationOsteoporosis International. London: Springer London Ltd, v. 23, n. 2, p. 467-473, 2012.
dc.identifier.doi10.1007/s00198-011-1539-8
dc.identifier.issn0937-941X
dc.identifier.lattes8568814008546317
dc.identifier.urihttp://hdl.handle.net/11449/39922
dc.identifier.wosWOS:000299306300007
dc.language.isoeng
dc.publisherSpringer London Ltd
dc.relation.ispartofOsteoporosis International
dc.relation.ispartofjcr3.856
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectDensitometryen
dc.subjectHistologyen
dc.subjectOsteoporosisen
dc.subjectOvariectomyen
dc.subjectRadiographic densitometryen
dc.titleDensitometry, radiography, and histological assessment of collagen as methods to evaluate femoral bones in an experimental model of osteoporosisen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer London Ltd
unesp.author.lattes8568814008546317
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araçatubapt
unesp.departmentOdontologia Infantil e Social - FOApt

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