Applied Protein and Molecular Techniques for Characterization of B Cell Neoplasms in Horses

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dc.contributor.authorBadial, Peres R. [UNESP]
dc.contributor.authorTallmadge, Rebecca L.
dc.contributor.authorMiller, Steven
dc.contributor.authorStokol, Tracy
dc.contributor.authorRichards, Kristy
dc.contributor.authorBorges, Alexandre S. [UNESP]
dc.contributor.authorFelippe, M. Julia B.
dc.contributor.institutionCornell Univ
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-11-26T15:28:09Z
dc.date.available2018-11-26T15:28:09Z
dc.date.issued2015-11-01
dc.description.abstractMature B cell neoplasms cover a spectrum of diseases involving lymphoid tissues (lymphoma) or blood (leukemia), with an overlap between these two presentations. Previous studies describing equine lymphoid neoplasias have not included analyses of clonality using molecular techniques. The objective of this study was to use molecular techniques to advance the classification of B cell lymphoproliferative diseases in five adult equine patients with a rare condition of monoclonal gammopathy, B cell leukemia, and concurrent lymphadenopathy (lymphoma/leukemia). The B cell neoplasms were phenotypically characterized by gene and cell surface molecule expression, secreted immunoglobulin (Ig) isotype concentrations, Ig heavy-chain variable (IGHV) region domain sequencing, and spectratyping. All five patients had hyperglobulinemia due to IgG1 or IgG4/7 monoclonal gammopathy. Peripheral blood leukocyte immunophenotyping revealed high proportions of IgG1- or IgG4/7-positive cells and relative T cell lymphopenia. Most leukemic cells lacked the surface B cell markers CD19 and CD21. IGHG1 or IGHG4/7 gene expression was consistent with surface protein expression, and secreted isotype and Ig spectratyping revealed one dominant monoclonal peak. The mRNA expression of the B cell-associated developmental genes EBF1, PAX5, and CD19 was high compared to that of the plasma cell-associated marker CD38. Sequence analysis of the IGHV domain of leukemic cells revealed mutated Igs. In conclusion, the protein and molecular techniques used in this study identified neoplastic cells compatible with a developmental transition between B cell and plasma cell stages, and they can be used for the classification of equine B cell lymphoproliferative disease.en
dc.description.affiliationCornell Univ, Coll Vet Med, Dept Clin Sci, Ithaca, NY 14853 USA
dc.description.affiliationUniv Estadual Paulista, Dept Vet Clin Sci, Sch Vet Med & Anim Sci, Sao Paulo, Brazil
dc.description.affiliationCornell Univ, Coll Vet Med, Dept Populat Med & Diagnost Sci, Ithaca, NY 14853 USA
dc.description.affiliationCornell Univ, Coll Vet Med, Dept Biomed Sci, Ithaca, NY 14853 USA
dc.description.affiliationUnespUniv Estadual Paulista, Dept Vet Clin Sci, Sch Vet Med & Anim Sci, Sao Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2010/08774-7
dc.format.extent1133-1145
dc.identifierhttp://dx.doi.org/10.1128/CVI.00374-15
dc.identifier.citationClinical And Vaccine Immunology. Washington: Amer Soc Microbiology, v. 22, n. 11, p. 1133-1145, 2015.
dc.identifier.doi10.1128/CVI.00374-15
dc.identifier.fileWOS:000363814200001.pdf
dc.identifier.issn1556-6811
dc.identifier.urihttp://hdl.handle.net/11449/158571
dc.identifier.wosWOS:000363814200001
dc.language.isoeng
dc.publisherAmer Soc Microbiology
dc.relation.ispartofClinical And Vaccine Immunology
dc.relation.ispartofsjr1,320
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleApplied Protein and Molecular Techniques for Characterization of B Cell Neoplasms in Horsesen
dc.typeArtigo
dcterms.rightsHolderAmer Soc Microbiology
unesp.author.lattes9643433706163946[6]
unesp.author.orcid0000-0001-6256-8089[6]

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