New primers for the amplification and sequencing of nuclear loci in a taxonomically wide set of reptiles and amphibians

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dc.contributor.authorPinho, Catarina
dc.contributor.authorRocha, Sara
dc.contributor.authorCarvalho, Bruno M.
dc.contributor.authorLopes, Susana
dc.contributor.authorMourao, Sofia
dc.contributor.authorVallinoto, Marcelo
dc.contributor.authorBrunes, Tuliana O.
dc.contributor.authorHaddad, Celio Fernando Baptista [UNESP]
dc.contributor.authorGoncalves, Helena
dc.contributor.authorSequeira, Fernando
dc.contributor.authorFerrand, Nuno
dc.contributor.institutionUniv Porto
dc.contributor.institutionUniv Vigo
dc.contributor.institutionFed Univ Para
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:59:57Z
dc.date.available2014-05-20T13:59:57Z
dc.date.issued2010-09-01
dc.description.abstractWe report new primers for the amplification and sequencing of 11 nuclear markers in squamate reptiles and anuran amphibians (five in squamates, six in anurans). Ten out of the 11 loci are introns (three of which are linked) that were amplified using an exon-primed, intron-crossing (EPIC) PCR strategy, whereas an eleventh locus spans part of a protein-coding gene. Squamate and anuran primers were initially developed for Lacerta schreiberi (Squamata: Lacertidae) and Pelodytes spp. (Anura: Pelodytidae), respectively. Cross-species amplification of the squamate markers was evaluated in four genera representing two additional families, whereas for anurans three genera corresponding to three additional families were tested. Three out of the five loci were successfully sequenced in all squamate taxa tested. Cross-amplification of the six anuran markers had lower, but still significant, success. We predict these markers will be of great utility for both population genetics and phylogenetic studies.en
dc.description.affiliationUniv Porto, CIBIO, Ctr Invest Biodiversidade & Recursos Genet, P-4485661 Vairao, Portugal
dc.description.affiliationUniv Porto, Dept Zool & Antropol, Fac Ciencias, P-4169007 Oporto, Portugal
dc.description.affiliationUniv Vigo, Dept Bioquim Genet & Inmunol, Fac Biol, Vigo 36310, Spain
dc.description.affiliationFed Univ Para, BR-68000000 Braganca, Para, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Zool, Inst Biociencias, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Zool, Inst Biociencias, BR-13506900 Rio Claro, SP, Brazil
dc.description.sponsorshipFundação para a Ciência e a Tecnologia (FCT)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFCT: POCI/BIA-BDE/60911/2004
dc.description.sponsorshipIdFCT: PTDC/BIA-BDE/69769/2006
dc.description.sponsorshipIdFCT: CAPES/FCT 244/09
dc.description.sponsorshipIdFCT: SFRH/BPD/28869/2006
dc.description.sponsorshipIdFCT: SFRH/BPD/26555/2006
dc.description.sponsorshipIdFCT: SFRH/BPD/27134/2006
dc.description.sponsorshipIdFCT: SFRH/BD/17541/2004
dc.description.sponsorshipIdCAPES: 1362-07-0
dc.description.sponsorshipIdBI/PTDC/BIA-BDE/69769/2006
dc.description.sponsorshipIdBI/PTDC/BIA-QOR/71492/2006
dc.format.extent181-185
dc.identifierhttp://dx.doi.org/10.1007/s12686-009-9126-4
dc.identifier.citationConservation Genetics Resources. Dordrecht: Springer, v. 2, p. 181-185, 2010.
dc.identifier.doi10.1007/s12686-009-9126-4
dc.identifier.issn1877-7252
dc.identifier.lattes0458077399058762
dc.identifier.urihttp://hdl.handle.net/11449/21202
dc.identifier.wosWOS:000290413000044
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofConservation Genetics Resources
dc.relation.ispartofjcr0.742
dc.relation.ispartofsjr0,430
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectAnuraen
dc.subjectSquamataen
dc.subjectNuclear sequence markersen
dc.subjectIntronsen
dc.titleNew primers for the amplification and sequencing of nuclear loci in a taxonomically wide set of reptiles and amphibiansen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.lattes0458077399058762[8]
unesp.author.orcid0000-0002-7044-5764[8]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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Artigos - Zoologia - IBRC