Effect of berberine associated with photodynamic therapy in cell lines

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dc.contributor.authorOliveira, P. M.
dc.contributor.authorLopes, T. Z. [UNESP]
dc.contributor.authorTedesco, A. C.
dc.contributor.authorRahal, P. [UNESP]
dc.contributor.authorCalmon, M. F. [UNESP]
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2021-06-25T10:36:03Z
dc.date.available2021-06-25T10:36:03Z
dc.date.issued2020-12-01
dc.description.abstractCervical cancer is a serious worldwide health problem. In view of the potentially harmful effects of current conventional therapies, photodynamic therapy may be an option as it is a minimally invasive therapy and can promote selective cytotoxic activity for neoplastic cells in the target tissue., Berberine (BBR) as an isolated molecule is a natural compound that has antineoplastic properties and potential action as a photosensitizer agent. The purpose of this study was to evaluate the use of berberine as a photosensitizer in photodynamic therapy (PDT) protocols and observe the effects produced by this association in cervical carcinoma cells and in immortalized keratinocytes. Incubation with 2.5 μM berberine promoted less than 10 % of cellular death in both cell lines studied. In addition, by fluorescence microscopy, we demonstrated that berberine was internalized by the cells, and after a period of 48 h, it was still present in the intracellular environment preferentially localized in the cytoplasm. After photodynamic therapy using berberine as a photosensitizer and visible light activation at 447 (±10) nm, we observed a phototoxic effect, which resulted in 19.84 % cell viability for Caski cells and 47.22 % cell viability for HaCaT. Treatment with berberine associated with photodynamic therapy promoted an increase in the production of reactive species of oxygen (ROS) and caspase-3 activity, indicating a preferential cell death mechanism by caspase-dependent apoptosis. Therefore, we demonstrated that berberine is an efficient photosensitizer and that its association with photodynamic therapy may be a potential anticancer treatment strategy for cervical cancer.en
dc.description.affiliationTechnological Development Center (Centro de Desenvolvimento Tecnológico – ®CDTec) Federal University of Pelotas (Universidade Federal de Pelotas-UFPel)
dc.description.affiliationInstitute of Biosciences Humanities and Exact Sciences (IBILCE) Department of Biology São Paulo State University (UNESP)
dc.description.affiliationDepartment of Chemistry Center of Nanotechnology and Tissue Engineering-Photobiology and Photomedicine Research Group Faculty of Philosophy Sciences and Letters of Ribeirao Preto University of Sao Paulo
dc.description.affiliationUnespInstitute of Biosciences Humanities and Exact Sciences (IBILCE) Department of Biology São Paulo State University (UNESP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2013/50181-1
dc.description.sponsorshipIdFAPESP: 2015/16660-5
dc.identifierhttp://dx.doi.org/10.1016/j.pdpdt.2020.102045
dc.identifier.citationPhotodiagnosis and Photodynamic Therapy, v. 32.
dc.identifier.doi10.1016/j.pdpdt.2020.102045
dc.identifier.issn1873-1597
dc.identifier.issn1572-1000
dc.identifier.scopus2-s2.0-85092532188
dc.identifier.urihttp://hdl.handle.net/11449/206661
dc.language.isoeng
dc.relation.ispartofPhotodiagnosis and Photodynamic Therapy
dc.sourceScopus
dc.subjectBerberine
dc.subjectCell death
dc.subjectCervical cancer
dc.subjectNatural compound
dc.subjectPhotodynamic therapy
dc.titleEffect of berberine associated with photodynamic therapy in cell linesen
dc.typeArtigo

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