Carbamide Peroxide Determination in Tooth Whitening Using a Reagentless HRP-Biosensor

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Data

2009-01-01

Orientador

Coorientador

Pós-graduação

Curso de graduação

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Título de Volume

Editor

Taylor & Francis Inc

Tipo

Artigo

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Acesso restrito

Resumo

A rapid and effective method for carbamide peroxide (CP) quantification in pharmaceuticals is proposed. The reagentless biosensor was prepared by using lyophilized turnip extract as horseradish peroxidase (HRP) enzyme source. The biosensor presented the best performance. The measurements were carried out in 0.1mol L-1 Pipes buffer (pH 6.0). At-100mV vs. Ag|AgCl, a linear response range from 3.0 to 25.0mmol L-1 was obtained. The quantification and detection limits were 1.2 and 0.4mmol L-1, respectively, and the response time was 0.5s. The biosensor repeatability, storage, and lifetime were excellent, allowing a satisfactory CP quantification in real pharmaceutical samples, when compared with those obtained by the official method.

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Inglês

Como citar

Analytical Letters. Philadelphia: Taylor & Francis Inc, v. 42, n. 2, p. 352-365, 2009.

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