Acetylcholine receptors of the neuromuscular junctions present normal distribution after peripheral nerve injury and repair through nerve guidance associated with fibrin biopolymer

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dc.contributor.authorLeite, Ana Paula Silveira [UNESP]
dc.contributor.authorPinto, Carina Guidi [UNESP]
dc.contributor.authorTibúrcio, Felipe Cantore [UNESP]
dc.contributor.authorMuller, Kevin Silva [UNESP]
dc.contributor.authorPadovani, Carlos Roberto [UNESP]
dc.contributor.authorBarraviera, Benedito [UNESP]
dc.contributor.authorJunior, Rui Seabra Ferreira [UNESP]
dc.contributor.authorLeal, Claudenete Vieira
dc.contributor.authorMatsumura, Cintia Yuri [UNESP]
dc.contributor.authorMatheus, Selma Maria Michelin [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2023-07-29T13:31:44Z
dc.date.available2023-07-29T13:31:44Z
dc.date.issued2023-02-01
dc.description.abstractPeripheral nerve injuries (PNI) lead to alterations in the Agrin-LRP4-MuSK pathway. This results in disaggregation of AChRs and change from epsilon (mature, innervated) to gamma (immature, denervated) subunit. Tubulization technique has been shown to be effective for PNI repair and it also allows the use of adjuvants, such as fibrin biopolymer (FB). This study evaluated the effect of the association of tubulization with FB after PNI on AChRs and associated proteins. Fifty-two adults male Wistar rats were used, distributed in 4 experimental groups: Sham Control (S), Denervated Control (D); Tubulization (TB) and Tubulization + Fibrin Biopolymer (TB+FB). Catwalk was performed every 15 days. Ninety days after surgery the right soleus muscles and ischiatic nerves were submitted to the following analyses: (a) morphological and morphometric analysis of AChRs by confocal microscopy; (b) morphological and morphometric analysis of the ischiatic nerve; (c) protein quantification of AChRs: alpha, gama, and epsilon, of Schwann cells, agrin, LRP4, MuSK, rapsyn, MMP3, MyoD, myogenin, MURF1 and atrogin-1. The main results were about the NMJs that in the TB+FB group presented morphological and morphometric approximation (compactness index; area of the AChRs and motor plate) to the S group. In addition, there were also an increase of S100 and AChRε protein expression and a decrease of MyoD. These positive association resulted in AChRs stabilization that potentiate the neuromuscular regeneration, which strengthens the use of TB for severe injuries repair and the beneficial effect of FB, along with tubulization technique.en
dc.description.affiliationMedical School São Paulo State University (Unesp), SP
dc.description.affiliationDivision of Anatomy Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (Unesp), SP
dc.description.affiliationDivision of Biostatistics Department of Biostatistics Vegetal Biology Parasitology and Zoology Institute of Biosciences São Paulo State University (Unesp), SP
dc.description.affiliationCenter for the Study of Venoms and Venomous Animals (Cevap) São Paulo State University (Unesp), SP
dc.description.affiliationSchool of Mechanical Engineering University of Campinas (Unicamp), SP
dc.description.affiliationUnespMedical School São Paulo State University (Unesp), SP
dc.description.affiliationUnespDivision of Anatomy Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (Unesp), SP
dc.description.affiliationUnespDivision of Biostatistics Department of Biostatistics Vegetal Biology Parasitology and Zoology Institute of Biosciences São Paulo State University (Unesp), SP
dc.description.affiliationUnespCenter for the Study of Venoms and Venomous Animals (Cevap) São Paulo State University (Unesp), SP
dc.format.extent345-361
dc.identifierhttp://dx.doi.org/10.1016/j.injury.2022.11.047
dc.identifier.citationInjury, v. 54, n. 2, p. 345-361, 2023.
dc.identifier.doi10.1016/j.injury.2022.11.047
dc.identifier.issn1879-0267
dc.identifier.issn0020-1383
dc.identifier.scopus2-s2.0-85143544360
dc.identifier.urihttp://hdl.handle.net/11449/248000
dc.language.isoeng
dc.relation.ispartofInjury
dc.sourceScopus
dc.subjectAcetylcholine receptors
dc.subjectFibrin biopolymer
dc.subjectNeuromuscular junction
dc.subjectPeripheral nerve injury
dc.subjectPolycaprolactone
dc.titleAcetylcholine receptors of the neuromuscular junctions present normal distribution after peripheral nerve injury and repair through nerve guidance associated with fibrin biopolymeren
dc.typeArtigo
unesp.author.orcid0000-0003-2660-6089 0000-0003-2660-6089[3]
unesp.author.orcid0000-0001-9774-1435 0000-0001-9774-1435[4]
unesp.author.orcid0000-0002-7237-7021[9]

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Botucatu - CEVAP - Centro de Estudos de Venenos e Animais Peçonhentos