Effect of EDTA and QMIX ultrasonic activation on the reduction of microorganisms and endotoxins in ex vivo human root canals

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dc.contributor.authorMatos, Felipe de Souza [UNESP]
dc.contributor.authorKhoury, Rayana Duarte [UNESP]
dc.contributor.authorCarvalho, Cláudio Antonio Talge [UNESP]
dc.contributor.authorMartinho, Frederico Canato
dc.contributor.authorBresciani, Eduardo [UNESP]
dc.contributor.authorValera, Marcia Carneiro [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionSchool of Dentistry
dc.date.accessioned2019-10-06T17:12:27Z
dc.date.available2019-10-06T17:12:27Z
dc.date.issued2019-05-01
dc.description.abstractThe present study aimed to compare the effectiveness of QMiX and 17% EDTA associated to passive ultrasonic irrigation (PUI) or manual agitation (MA) on the reduction of E. faecalis, E. coli and LPS from root canals. Forty single rooted human teeth were randomly divided into fourgroups(n=10),according to the final irrigation protocol: EDTA+MA,QMiX+MA,EDTA+PUI, QMiX+PUI. Sample collections were obtained from the root canal content immediately before preparation (baseline-S1), after instrumentation (S2), after final irrigation protocol (S3) and 7 days after instrumentation and final irrigation (S4). The antimicrobial effectivity and on endotoxin content were analyzed by culture procedure (CFU/mL) and LAL assay (EU/ mL), respectively. The results were statistically analyzed by Kruskal-Wallis and Friedman test (α=5%). QMiX+MA and QMiX+PUI reduced 100% of E. coli and E. faecalis bacteria and also preventedE. faecalis regrowthat S4. EDTAsignificantly reducedE. coli, but itwas not effective in reducing E. faecalis. All protocols reduced EU/mL when compared to S1, however at S4 there was a significant reduction of EU/mL only in the QMiX+MA and QMiX+PUI groups in relation to S3 and S2, respectively. Final irrigation with QMiX associated with MA or PUI had superior antibacterial efficacy compared to EDTA, eliminating 100% of E. coli and E. faecalis strains. In addition, QMiX+PUI reduced 97.61% of the initial content of LPS.en
dc.description.affiliationDepartment of Restorative Dentistry Institute of Science and Technology UNESP-Universidade Estadual Paulista
dc.description.affiliationUniversity of Maryland School of Dentistry
dc.description.affiliationUnespDepartment of Restorative Dentistry Institute of Science and Technology UNESP-Universidade Estadual Paulista
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2014/ 25789-9
dc.format.extent220-226
dc.identifierhttp://dx.doi.org/10.1590/0103-6440201902470
dc.identifier.citationBrazilian Dental Journal, v. 30, n. 3, p. 220-226, 2019.
dc.identifier.doi10.1590/0103-6440201902470
dc.identifier.fileS0103-64402019000300220.pdf
dc.identifier.issn1806-4760
dc.identifier.issn0103-6440
dc.identifier.lattes9304600166583100
dc.identifier.orcid0000-0003-0987-5594
dc.identifier.scieloS0103-64402019000300220
dc.identifier.scopus2-s2.0-85067513563
dc.identifier.urihttp://hdl.handle.net/11449/190415
dc.language.isoeng
dc.relation.ispartofBrazilian Dental Journal
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBacteria
dc.subjectEndotoxin
dc.subjectPassive ultrasonic irrigation
dc.subjectQMix root canal irrigant
dc.titleEffect of EDTA and QMIX ultrasonic activation on the reduction of microorganisms and endotoxins in ex vivo human root canalsen
dc.typeArtigo
unesp.author.lattes9304600166583100[3]
unesp.author.orcid0000-0001-5619-3831[1]
unesp.author.orcid0000-0001-6403-8348[2]
unesp.author.orcid0000-0003-0987-5594[3]
unesp.author.orcid0000-0002-4140-340X[4]
unesp.author.orcid0000-0001-9299-8792[5]
unesp.author.orcid0000-0001-7398-6438[6]

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