Treatment of bisphosphonate-related osteonecrosis using platelet-rich plasma: microtomographic, microscopic, and immunohistochemical analyses

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dc.contributor.authorCardoso, Camila Lopes
dc.contributor.authorCurra, Cláudia
dc.contributor.authorCuri, Marcos Martins
dc.contributor.authorMatsumoto, Mariza Akemi
dc.contributor.authorArgentino, Camila Dionísio
dc.contributor.authorFranzolin, Solange De Oliveira Braga
dc.contributor.authorConstantino, Dulce
dc.contributor.authorBarbosa, Daniela Nicolielo
dc.contributor.authorFerreira JÚnior, Osny
dc.contributor.institutionUniversidade do Sagrado Coração Department of Oral Surgery
dc.contributor.institutionHospital Santa Catarina Department of Stomatology
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade do Sagrado Coração Department of Health Sciences
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2019-10-03T17:31:39Z
dc.date.available2019-10-03T17:31:39Z
dc.date.issued2019
dc.description.abstractAbstract The present study aimed to investigate the use of platelet-rich plasma (PRP) on tooth extraction sites in rats treated with bisphosphonates. Thirty Albinus Wistar male rats were administered 0.035 mg/kg zoledronic acid intravenously for 8 weeks, divided into four administrations with a 2-week interval between each application, after which their upper right central incisors were extracted to induce the development of bisphosphonate-related osteonecrosis of the jaw (BRONJ). The samples were divided into the following two groups: Group 1 (G1) underwent marginal resection of BRONJ followed by the use of PRP, while Group 2 (G2) underwent resection of BRONJ but without the use of PRP. The treatment groups were evaluated after 14, 28, and 42 days. Clinical, microtomographic, microscopic, and immunohistochemical (IHC) evaluations were performed. Microtomography results revealed no significant difference between the groups (p <0.05) in any time period. Histomorphometric analysis showed increased bone formation over time for both groups (p < 0.001). G1 demonstrated a greater amount of new bone formation than G2 at 28 and 42 days (p < 0.001), with G1 presenting greater vascularization and a slightly higher VEGF expression. For both groups, RANKL/OPG expression levels were sufficient as a parameter for indicating the rate of bone remodeling in a previously treated area of osteonecrosis groups. Taken together, our findings indicated that the use of PRP improves the resolution process of BRONJ.en
dc.description.affiliationUniversidade do Sagrado Coração Department of Oral Surgery
dc.description.affiliationHospital Santa Catarina Department of Stomatology
dc.description.affiliationUniversidade Estadual Paulista Department of Histology
dc.description.affiliationUniversidade do Sagrado Coração Department of Health Sciences
dc.description.affiliationUniversidade de São Paulo Department of Oral Surgery
dc.description.affiliationUnespUniversidade Estadual Paulista Department of Histology
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1590/1807-3107bor-2019.vol33.0050
dc.identifier.citationBrazilian Oral Research. Sociedade Brasileira de Pesquisa Odontológica - SBPqO, v. 33, p. -, 2019.
dc.identifier.doi10.1590/1807-3107bor-2019.vol33.0050
dc.identifier.fileS1806-83242019000100242.pdf
dc.identifier.issn1806-8324
dc.identifier.lattes8621510886887389
dc.identifier.scieloS1806-83242019000100242
dc.identifier.urihttp://hdl.handle.net/11449/183790
dc.language.isoeng
dc.publisherSociedade Brasileira de Pesquisa Odontológica - SBPqO
dc.relation.ispartofBrazilian Oral Research
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectDisphosphonatesen
dc.subjectPlatelet-Rich Plasmaen
dc.subjectWound Healingen
dc.titleTreatment of bisphosphonate-related osteonecrosis using platelet-rich plasma: microtomographic, microscopic, and immunohistochemical analysesen
dc.typeArtigo
unesp.author.lattes8621510886887389

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