OPTIMIZED METHOD FOR DNA EXTRACTION AND PCR AMPLIFICATION IN AROEIRA TREE

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dc.contributor.authorSilva, Jailson Do Nascimento
dc.contributor.authorLinhares, Amanda Camila Dos Santos
dc.contributor.authorPalmieri, Dario Abel [UNESP]
dc.contributor.authorCosta, Marcones Ferreira
dc.contributor.authorde SÁ, Gisele Holanda
dc.contributor.authorGomes, Maria Fernanda da Costa
dc.contributor.authorDos Santos, Michelli Ferreira
dc.contributor.authorFeitoza, Lidiane de Lima
dc.contributor.authorValente, Sérgio Emílio Dos Santos
dc.contributor.institutionInstituto Federal do Piauí
dc.contributor.institutionUniversidade Federal do Piauí
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Estadual do Piauí
dc.date.accessioned2023-07-29T16:08:06Z
dc.date.available2023-07-29T16:08:06Z
dc.date.issued2023-01-01
dc.description.abstractMolecular markers are important tools in the characterization of plant genetic diversity and can provide support for conservation strategies for endangered populations. The different molecular techniques involve the evaluation of many individuals; therefore, it is crucial to have fast, efficient, and inexpensive methods for DNA extraction. Given the importance of the Aroeira (Myracrodruon urundeuva Fr. All.) it is pertinent to optimize a protocol that allows the obtainment of intact and pure DNA, aiming to assist conservation strategies for this species that is threatened with extinction. Thus, this study aimed to compare five DNA extraction methods: Dellaporta et al. (1983), Doyle and Doyle (1987) modified, Ferreira and Grattapaglia (1995), Romano and Brasileiro (2015), and Khanuja et al. (1999) and optimize the most efficient protocol for M. urundeuva. The modified DNA extraction protocol proposed by Doyle and Doyle (1987), using 100 mg of leaf tissue and 6 µl of β-mercaptoethanol was the protocol that presented the sharpest bands after DNA electrophoresis and after the reactions of amplification employing Polymerase Chain Reaction (PCR). Therefore, it is suggested to use the protocol described by Doyle and Doyle (1987) modified for the extraction of DNA from young M. urundeuva leaves to carry out techniques involving molecular markers.en
dc.description.affiliationDepartment of Education Instituto Federal do Piauí, Piauí
dc.description.affiliationDepartment of Biology Universidade Federal do Piauí, Piauí
dc.description.affiliationDepartment of Biotechnology Universidade Estadual Paulista (UNESP), São Paulo
dc.description.affiliationUniversidade Federal do Piauí, Piauí
dc.description.affiliationPostgraduate Program in Genetics and Breeding Universidade Federal do Piauí, Piauí
dc.description.affiliationDepartment of Biology Universidade Estadual do Piauí, Piauí
dc.description.affiliationUnespDepartment of Biotechnology Universidade Estadual Paulista (UNESP), São Paulo
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.identifierhttp://dx.doi.org/10.14393/BJ-v39n0a2023-62577
dc.identifier.citationBioscience Journal, v. 39.
dc.identifier.doi10.14393/BJ-v39n0a2023-62577
dc.identifier.issn1981-3163
dc.identifier.issn1516-3725
dc.identifier.scopus2-s2.0-85149714071
dc.identifier.urihttp://hdl.handle.net/11449/249748
dc.language.isoeng
dc.relation.ispartofBioscience Journal
dc.sourceScopus
dc.subjectCTAB
dc.subjectDNA isolation
dc.subjectMyracrodruon urundeuva
dc.titleOPTIMIZED METHOD FOR DNA EXTRACTION AND PCR AMPLIFICATION IN AROEIRA TREEen
dc.typeArtigo
unesp.departmentCiências Biológicas - FCLASpt

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