In vitro susceptibility of oral Candida albicans strains to different pH levels and calcium hydroxide saturated aqueous solution

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dc.contributor.authorWeckwerth, Paulo Henrique
dc.contributor.authorCarnietto, Cristiane
dc.contributor.authorWeckwerth, Ana Carolina Villas Boas
dc.contributor.authorDuarte, Marco Antonio Hungaro
dc.contributor.authorKuga, Milton Carlos [UNESP]
dc.contributor.authorVivan, Rodrigo Ricci
dc.contributor.institutionUniversidade do Sagrado Coração (USC)
dc.contributor.institutionInstituto Lauro de Souza Lima (ILSL)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:26:53Z
dc.date.available2014-05-27T11:26:53Z
dc.date.issued2012-07-30
dc.description.abstractCandida albicans is present in the oral cavity and in the whole digestive tract of humans and other animals, being frequently related to endodontic treatment failure. The present study determined the incidence of C. albicans in the oral cavity and the susceptibility of isolates to different pH values and saturated calcium hydroxide aqueous solution at pH 12.5. Sixty-five patients attending the Endodontic Clinic at the Sagrado Coração University participated in the study. The collected samples were cultivated in selective media for C. albicans and the isolates were tested in terms of resistance to both alkaline pH and saturated aqueous solution of calcium hydroxide. In relation to time variables, yeast viability was assessed by the Sabouraud's agar culture and fluorescein diacetate and ethidium bromide fluorescent staining method. Results from the different pHs and experimental times, including those from different techniques measuring fungal viability, were compared using the chi-square and Fisher's exact tests (α=0.05). The yeasts became completely inviable after 48 h of contact with the calcium hydroxide solution. On the other hand, when exposed to the alkaline culture broth, the yeasts were found to be viable at pHs 9.5 and 10.5 for up to 7 days. In conclusion, C. albicans can only be completely inhibited by direct contact with saturated calcium hydroxide aqueous solution after 48 h of exposure.en
dc.description.affiliationDepartment of Biological Sciences Center of Health Sciences USC - Sagrado Coração University, Bauru, SP
dc.description.affiliationLauro de Souza Lima Hospital, Bauru, SP
dc.description.affiliationDepartment of Operative Dentistry Dental Materials and Endodontics Bauru Dental School, USP - Univeristy of São Paulo, Bauru, SP
dc.description.affiliationDepartment of Restorative Dentistry Araraquara Dental School Universidade Estadual Paulista (UNESP), Araraquara, SP
dc.description.affiliationDepartment of Dentistry Center of Health Sciences USC - Sagrado Coração University, Bauru, SP
dc.description.affiliationUnespDepartment of Restorative Dentistry Araraquara Dental School Universidade Estadual Paulista (UNESP), Araraquara, SP
dc.format.extent192-198
dc.identifierhttp://dx.doi.org/10.1590/S0103-64402012000300002
dc.identifier.citationBrazilian Dental Journal, v. 23, n. 3, p. 192-198, 2012.
dc.identifier.doi10.1590/S0103-64402012000300002
dc.identifier.file2-s2.0-84864226344.pdf
dc.identifier.issn0103-6440
dc.identifier.issn1806-4760
dc.identifier.lattes1627568419811788
dc.identifier.scieloS0103-64402012000300002
dc.identifier.scopus2-s2.0-84864226344
dc.identifier.urihttp://hdl.handle.net/11449/73455
dc.language.isoeng
dc.relation.ispartofBrazilian Dental Journal
dc.relation.ispartofsjr0,476
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCalcium hydroxide
dc.subjectCandida albicans
dc.subjectOral cavity
dc.subjectSusceptibility
dc.subjectbiomedical and dental materials
dc.subjectcalcium hydroxide
dc.subjectdrug effect
dc.subjecthuman
dc.subjectisolation and purification
dc.subjectmicrobial sensitivity test
dc.subjectmicrobiology
dc.subjectmouth
dc.subjectpH
dc.subjectstaining
dc.subjectyeast
dc.subjectCalcium Hydroxide
dc.subjectHumans
dc.subjectHydrogen-Ion Concentration
dc.subjectMicrobial Sensitivity Tests
dc.subjectMouth
dc.subjectRoot Canal Irrigants
dc.subjectStaining and Labeling
dc.subjectYeasts
dc.titleIn vitro susceptibility of oral Candida albicans strains to different pH levels and calcium hydroxide saturated aqueous solutionen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/bdj/iaboutj.htm
unesp.author.lattes1627568419811788
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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