An optimised electrochemical biosensor for the label-free detection of C-reactive protein in blood
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Elsevier
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Resumo
C-reactive protein (CRP) is an acute phase protein whose levels are increased in many disorders. There exists, in particular, a great deal of interest in the correlation between blood serum levels and the severity of risk for cardiovascular disease. A sensitive, label-free, non-amplified and reusable electrochemical impedimetric biosensor for the detection of CRP in blood serum was developed herein based on controlled and coverage optimised antibody immobilization on standard polycrystalline gold electrodes. Charge transfer resistance changes were highly target specific, linear with log. CRP. concentration across a 0.5-50. nM range and associated with a limit of detection of 176. pM. Significantly, the detection limits are better than those of current CRP clinical methods and the assays are potentially cheap, relatively automated, reusable, multiplexed and highly portable. The generated interfaces were capable not only of comfortably quantifying CRP across a clinically relevant range of concentrations but also of doing this in whole blood serum with interfaces that were, subsequently, reusable. The importance of optimising receptor layer resistance in maximising assay sensitivity is also detailed. © 2012.
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Biosensor Blood C-reactive protein Electrochemical impedance spectroscopy Resistance Self-assembly Acute phase proteins Antibody immobilization Blood serum C-reactive proteins Cardio-vascular disease Charge transfer resistance Clinical methods Detection limits Electrochemical biosensor Highly-portable Label free Label-free detection Limit of detection Polycrystalline gold Receptor layers Whole blood Assays Charge transfer Chemical detection Electric resistance Proteins Self assembly Biosensors C reactive protein polyclonal antibody biosensor blood analysis controlled study electric resistance electrochemical detection electrochemical impedance spectroscopy human laboratory automation limit of detection process development process optimization protein blood level protein determination protein immobilization quantitative analysis sensitivity and specificity validation process Biosensing Techniques C-Reactive Protein Cardiovascular Diseases Dielectric Spectroscopy Humans Immunoassay Limit of Detection Serum
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Inglês
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Biosensors and Bioelectronics, v. 39, n. 1, p. 94-98, 2013.
