Interactions between Candida albicans and Candida glabrata in biofilms: Influence of the strain type, culture medium and glucose supplementation

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dc.contributor.authorHosida, Thayse Yumi [UNESP]
dc.contributor.authorCavazana, Thamires Priscila [UNESP]
dc.contributor.authorHenriques, Mariana
dc.contributor.authorPessan, Juliano Pelim [UNESP]
dc.contributor.authorDelbem, Alberto Carlos Botazzo [UNESP]
dc.contributor.authorMonteiro, Douglas Roberto [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Minho
dc.contributor.institutionUniversity of Western São Paulo (UNOESTE)
dc.date.accessioned2018-12-11T17:35:28Z
dc.date.available2018-12-11T17:35:28Z
dc.date.issued2018-04-01
dc.description.abstractThe relationship among Candida species may be influenced by several factors. Thus, this study evaluated the interactions between Candida albicans and Candida glabrata in biofilms, varying the strain type, culture medium and glucose supplementation. Biofilms were formed for 48 hours in Sabouraud dextrose broth (SDB) or RPMI 1640, supplemented with 0%, 1% or 5% glucose. Each strain of C. albicans was combined with two strains of C. glabrata, generating four biofilm associations, which were quantified by colony-forming units (CFUs), total biomass and metabolic activity. Data were analysed by ANOVA and Tukey's HSD test (α = 0.05). For CFUs, all associations were classified as indifferent for biofilms formed in RPMI 1640, while for SDB the interactions were antagonistic for C. albicans and indifferent for C. glabrata. The association of reference strains resulted in a dual-species biofilm with biomass significantly higher than that observed for each single biofilm developed in SDB. The metabolic activity of dual-species biofilms did not significantly differ from that found for single ones, except for co-culture of the reference strains. Glucose supplementation and culture media had a significant influence on all parameters. In conclusion, the strain type, culture medium and glucose supplementation influenced the interactions between C. albicans and C. glabrata.en
dc.description.affiliationDepartment of Pediatric Dentistry and Public Health São Paulo State University (Unesp) School of Dentistry
dc.description.affiliationCEB Centre of Biological Engineering LIBRO – Laboratório de Investigação em Biofilmes Rosário Oliveira University of Minho
dc.description.affiliationGraduate Program in Dentistry (GPD - Master’s Degree) University of Western São Paulo (UNOESTE)
dc.description.affiliationUnespDepartment of Pediatric Dentistry and Public Health São Paulo State University (Unesp) School of Dentistry
dc.format.extent270-278
dc.identifierhttp://dx.doi.org/10.1111/myc.12738
dc.identifier.citationMycoses, v. 61, n. 4, p. 270-278, 2018.
dc.identifier.doi10.1111/myc.12738
dc.identifier.issn1439-0507
dc.identifier.issn0933-7407
dc.identifier.scopus2-s2.0-85040673826
dc.identifier.urihttp://hdl.handle.net/11449/179512
dc.language.isoeng
dc.relation.ispartofMycoses
dc.relation.ispartofsjr1,069
dc.relation.ispartofsjr1,069
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectbiofilms
dc.subjectCandida albicans
dc.subjectCandida glabrata
dc.subjectmicrobial interactions
dc.titleInteractions between Candida albicans and Candida glabrata in biofilms: Influence of the strain type, culture medium and glucose supplementationen
dc.typeArtigo
unesp.author.lattes0421571920582994[5]
unesp.author.orcid0000-0003-0317-4877[3]
unesp.author.orcid0000-0002-1550-3933[4]
unesp.author.orcid0000-0002-8159-4853[5]
unesp.author.orcid0000-0001-5229-5259[6]

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