Ultrastructure of last larval instar fat body cells of Pachycondyla (= Neoponera) villosa (Formicidae: Ponerinae): cytochemical and chemical analysis

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dc.contributor.authorJosé Zara, Fernando
dc.contributor.authorHenrique Caetano, Flávio [UNESP]
dc.contributor.authorCabrera, Aivlé Cecilia G.
dc.contributor.authorJaffé, Klaus
dc.contributor.institutionUNIMEP
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidad Simón Bolívar (USB)
dc.date.accessioned2023-07-29T13:49:52Z
dc.date.available2023-07-29T13:49:52Z
dc.date.issued2003-01-01
dc.description.abstractThe ultrastructure of the fat body cells (trophocytes) of the last larval instar of Pachycondyla (D Neoponera) villosa is presented. The cytoplasm is restricted to the cell periphery and to the smaller strips among the vacuoles, protein granules, lipid droplets, and around the nucleus. Cytochemically, the presence of basic amino acids in the protein granules and in the nuclei was observed by using the ethanolic phosphotungstic acid technique (EPTA). The lipid droplets stained for unsaturated lipids. This result was further confi rmed by gas chromatography and mass spectrometry, where the unsaturated fatty acids were identifi ed as oleic and linoleic acids together with saturated fatty acids such as palmitic and stearic acid. Carbohydrates (glycogen) were also detected in the fat body. The glycogen is present as ¯ particles distributed among the lipid droplets and sometimes attached to them.en
dc.description.affiliationFaculdade de Ciências Matemáticas da Natureza e Tecnologia da Informaçao UNIMEP Campus Taqvaral, Rod. do Açúcar km 156, Piracicaba
dc.description.affiliationDepartamento de Biologia Instituto de Biociências de Rio Claro – UNESP, P.O. Box 199, SP
dc.description.affiliationLaboratorio de Comportamento Universidad Simón Bolívar (USB)
dc.description.affiliationUnespDepartamento de Biologia Instituto de Biociências de Rio Claro – UNESP, P.O. Box 199, SP
dc.identifierhttp://dx.doi.org/10.1163/157075603769682521
dc.identifier.citationAnimal Biology, v. 53, n. 1, 2003.
dc.identifier.doi10.1163/157075603769682521
dc.identifier.issn1570-7563
dc.identifier.issn1570-7555
dc.identifier.scopus2-s2.0-85151844738
dc.identifier.urihttp://hdl.handle.net/11449/248650
dc.language.isoeng
dc.relation.ispartofAnimal Biology
dc.sourceScopus
dc.subjectchromatography
dc.subjectcytochemistry
dc.subjectfat body
dc.subjectlarva
dc.subjectPachycondyla villosa
dc.subjectultrastructure
dc.titleUltrastructure of last larval instar fat body cells of Pachycondyla (= Neoponera) villosa (Formicidae: Ponerinae): cytochemical and chemical analysisen
dc.typeArtigo

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