The Genome of “Candidatus Liberibacter asiaticus” Is Highly Transcribed When Infecting the Gut of Diaphorina citri

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dc.contributor.authorDarolt, Josiane Cecília [UNESP]
dc.contributor.authorBento, Flavia de Moura Manoel
dc.contributor.authorMerlin, Bruna Laís
dc.contributor.authorPeña, Leandro
dc.contributor.authorCônsoli, Fernando Luis
dc.contributor.authorWulff, Nelson Arno [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionFundo de Defesa da Citricultura – Fundecitrus
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Politécnica de Valencia
dc.date.accessioned2022-04-29T08:45:37Z
dc.date.available2022-04-29T08:45:37Z
dc.date.issued2021-07-12
dc.description.abstractThe Asian citrus psyllid, Diaphorina citri, is the vector of the bacterium “Candidatus Liberibacter asiaticus” (Las), associated with the devastating, worldwide citrus disease huanglongbing. In order to explore the molecular interactions of this bacterium with D. citri during the vector acquisition process, cDNA libraries were sequenced on an Illumina platform, obtained from the gut of adult psyllids confined in healthy (H) and in Las-infected young shoots (Las) for different periods of times (I = 1/2 days, II = 3/4 days, and III = 5/6 days). In each sampling time, three biological replicates were collected, containing 100 guts each, totaling 18 libraries depleted in ribosomal RNA. Reads were quality-filtered and mapped against the Chinese JXGC Las strain and the Floridian strain UF506 for the analysis of the activity of Las genome and SC1, SC2, and type 3 (P-JXGC-3) prophages of the studied Las strain. Gene activity was considered only if reads of at least two replicates for each acquisition access period mapped against the selected genomes, which resulted in coverages of 44.4, 79.9, and 94.5% of the JXGC predicted coding sequences in Las I, Las II, and Las III, respectively. These genes indicate an active metabolism and increased expression according to the feeding time in the following functional categories: energy production, amino acid metabolism, signal translation, cell wall, and replication and repair of genetic material. Pilins were among the most highly expressed genes regardless of the acquisition time, while only a few genes from cluster I of flagella were not expressed. Furthermore, the prophage region had a greater coverage of reads for SC1 and P-JXGC-3 prophages and low coverage in SC2 and no indication of activity for the lysis cycle. This research presents the first descriptive analysis of Las transcriptome in the initial steps of the D. citri gut colonization, where 95% of Las genes were active.en
dc.description.affiliationInstituto de Química Universidade Estadual Paulista “Julio de Mesquita Filho” – UNESP
dc.description.affiliationDepartamento de Pesquisa Desenvolvimento Fundo de Defesa da Citricultura – Fundecitrus
dc.description.affiliationLaboratório de Interações em Insetos Departamento de Entomologia e Acarologia Escola Superior de Agricultura Luiz de Queiroz Universidade de São Paulo
dc.description.affiliationInstituto de Biologia Molecular y Celular de Plantas – Consejo Superior de Investigaciones Científicas Universidade Politécnica de Valencia
dc.description.affiliationUnespInstituto de Química Universidade Estadual Paulista “Julio de Mesquita Filho” – UNESP
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.identifierhttp://dx.doi.org/10.3389/fmicb.2021.687725
dc.identifier.citationFrontiers in Microbiology, v. 12.
dc.identifier.doi10.3389/fmicb.2021.687725
dc.identifier.issn1664-302X
dc.identifier.scopus2-s2.0-85111349716
dc.identifier.urihttp://hdl.handle.net/11449/231485
dc.language.isoeng
dc.relation.ispartofFrontiers in Microbiology
dc.sourceScopus
dc.subjectACP
dc.subjectgreening
dc.subjectHLB
dc.subjectLas
dc.subjectmetatranscriptomics
dc.subjectprophage
dc.subjectpsyllid
dc.titleThe Genome of “Candidatus Liberibacter asiaticus” Is Highly Transcribed When Infecting the Gut of Diaphorina citrien
dc.typeArtigo
unesp.author.orcid0000-0003-3091-8747[1]

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Rio Claro - CEIS - Centro de Estudos de Insetos Sociais