The influence of cardiopulmonary bypass operation on the biodistribution of 99mTc-HMPAO-labelled granulocytes – Evaluation in pigs by planar scintigraphy and section-analyses

Página do item simplificado
dc.contributor.authorHappel, C.
dc.contributor.authorMargraf, S.
dc.contributor.authorDiener, J.
dc.contributor.authorKranert, W. T.
dc.contributor.authorFrancischetti, I. [UNESP]
dc.contributor.authorBitu-Moreno, J. [UNESP]
dc.contributor.authorAckermann, H.
dc.contributor.authorMiddendorp, M.
dc.contributor.authorTheisen, A.
dc.contributor.authorMoritz, A.
dc.contributor.authorScholz, M.
dc.contributor.authorGruenwald, F.
dc.contributor.institutionKlinikum Johann Wolfgang Goethe Univ Frankfurt Ma
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Dusseldorf
dc.date.accessioned2014-05-20T15:33:44Z
dc.date.available2014-05-20T15:33:44Z
dc.date.issued2012-01-01
dc.description.abstractAim of the study was to evaluate the influence of an extra corporal perfusion (cardiopulmonary bypass operation - cpb) on activation and biodistribution of Tc-99m labelled granulocytes in pigs with and without inhibition of the granulocytes by a leukocyte inhibition module (LIM). The cpb is often related to an activation of granulocytes resulting in an inflammatory answer. The biological mechanisms are unsolved yet. First trials of our group showed that LIM may inhibit the activation of neutrophils and therefore antagonize a cpb-caused impairment of cardiac function. This study is the continuation of these experiments with a higher number of animals and the focus on scintigraphic imaging. Animals, material, methods: 39 German landrace pigs were subdivided into three groups: group A (control) median sternotomy without cpb, group B with cpb, group C with LIM in addition to cpb. After labelling with Tc-99m-HMPAO autologues granulocytes were reinjected. Subsequently to cpb, the animals underwent scintigraphic imaging. Quantification was performed with ROI evaluation and with tissue samples (section analysis) examined in a well counter. Results:A high uptake of Tc-99m-HMPAO was found in the liver. The count rates in brain, heart, lung, spleen and kidneys were far below. The amount of Tc-99m-activity in the organ related to the half life corrected administered activity [%] was for the tissue samples (group A/B/C): brain 0.01/0.02/0.03; lung 12.1/8.3/11.5; heart 0.35/0.54/0.42; kidney 1.24/0.87/1.02; spleen 4.0/4.0/4.5, liver 16.8/20.9/19.6. The count rates determined by ROI-evaluation of the scintigraphic images related to the total count rate in the image [%] were (group A/B/C): brain 1.1/0.9/1.0; lung 15.6/10.4/12.2; heart 4.0/3.5/3.4; kidney 4.0/2.9/3.2; spleen 7.6/7.7/9.5, liver 23.1/36.7/31.4. A significant difference in the tracer uptake between the groups could neither be detected by scintigraphic imaging nor evaluation of tissue samples. Conclusion: Scintigraphic imaging as well as section analysis showed a comparable biodistribution of the tracer. Therefore, the initial results of our group were not confirmed with a considerably higher number of animals. Neither cpb nor the use of the LIM influenced distribution of Tc-99m-labelled granulocytes in pigs significantly.en
dc.description.affiliationKlinikum Johann Wolfgang Goethe Univ Frankfurt Ma, Klin Nukl Med, Frankfurt, Germany
dc.description.affiliationUniv Estadual Paulista, Fac Med Marilia FAMEMA, Manilla, Brazil
dc.description.affiliationKlinikum Johann Wolfgang Goethe Univ Frankfurt Ma, Inst Biostat & Math Modellierung, Frankfurt, Germany
dc.description.affiliationKlinikum Johann Wolfgang Goethe Univ Frankfurt Ma, Zentrale Forsch Einrichtung, Frankfurt, Germany
dc.description.affiliationUniv Dusseldorf, Klin Unfall & Handchirurgie, Dusseldorf, Germany
dc.description.affiliationUnespUniv Estadual Paulista, Fac Med Marilia FAMEMA, Manilla, Brazil
dc.format.extent205-211
dc.identifierhttp://nuk.schattauer.de/en/contents/archivestandard/issue/1589/manuscript/17709/show.html
dc.identifier.citationNuklearmedizin-nuclear Medicine. Stuttgart: Schattauer Gmbh-verlag Medizin Naturwissenschaften, v. 51, n. 5, p. 205-211, 2012.
dc.identifier.issn0029-5566
dc.identifier.urihttp://hdl.handle.net/11449/42280
dc.identifier.wosWOS:000309900900008
dc.language.isodeu
dc.publisherSchattauer Gmbh-verlag Medizin Naturwissenschaften
dc.relation.ispartofNuklearmedizin-nuclear Medicine
dc.relation.ispartofjcr1.352
dc.relation.ispartofsjr0,543
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectTc-99m-HMPAOen
dc.subjectLeukocyte-Inhibition-Modulen
dc.subjectcardiopulmonary bypassen
dc.titleThe influence of cardiopulmonary bypass operation on the biodistribution of 99mTc-HMPAO-labelled granulocytes – Evaluation in pigs by planar scintigraphy and section-analysesen
dc.typeArtigo
dcterms.rightsHolderSchattauer Gmbh-verlag Medizin Naturwissenschaften

Arquivos

Licença do Pacote
Agora exibindo 1 - 2 de 2
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos