Neuroregeneration and immune response after neurorrhaphy are improved with the use of heterologous fibrin biopolymer in addition to suture repair alone

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dc.contributor.authorTibúrcio, Felipe Cantore [UNESP]
dc.contributor.authorMuller, Kevin Silva [UNESP]
dc.contributor.authorLeite, Ana Paula Silveira [UNESP]
dc.contributor.authorde Oliveira, Igor Rodrigues Araújo [UNESP]
dc.contributor.authorBarraviera, Benedito [UNESP]
dc.contributor.authorFerreira, Rui Seabra [UNESP]
dc.contributor.authorPadovani, Carlos Roberto [UNESP]
dc.contributor.authorPinto, Carina Guidi [UNESP]
dc.contributor.authorMatheus, Selma Maria Michelin [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T13:47:21Z
dc.date.available2023-07-29T13:47:21Z
dc.date.issued2023-06-01
dc.description.abstractIntroduction/Aims: Peripheral nerve injuries result in impaired neuromuscular interactions, leading to morphological and functional alterations. Adjuvant suture repair methods have been used to improve nerve regeneration and modulate the immune response. Heterologous fibrin biopolymer (HFB), a scaffold with adhesive properties, plays a critical role in tissue repair. The aim of this study is to evaluate neuroregeneration and immune response focusing on neuromuscular recovery, using suture-associated HFB for sciatic nerve repair. Methods: Forty adult male Wistar rats were distributed into four groups (n = 10): C (control), only sciatic nerve location; D (denervated), neurotmesis and 6-mm gap removal and fixation stumps in subcutaneous tissue; S (suture), neurotmesis followed by suture; and SB (suture + HFB), neurotmesis followed by suture and HFB. Analysis of M2 macrophages (CD206+), as well as the morphology and morphometry of nerves, soleus muscle, and neuromuscular junctions (NMJs), were performed at 7 and 30 days after surgery. Results: The SB group had the highest M2 macrophage area in both periods. After 7 days, SB was the only group similar to the C group regarding the number of axons; furthermore, after 30 days, the SB group was closer to the C group concerning blood vessel and central myonuclear numbers, NMJ angle, and connective tissue volume. After 7 days, increases in nerve area, as well as the number and area of blood vessels, were also observed in SB. Discussion: HFB potentiates the immune response, increases axonal regeneration, induces angiogenesis, prevents severe muscle degeneration, and assists in NMJ recovery. In conclusion, suture-associated HFB has major implications for improved peripheral nerve repair.en
dc.description.affiliationSão Paulo State University (Unesp) Medical School, São Paulo
dc.description.affiliationDivision of Anatomy Department of Structural and Functional Biology São Paulo State University (Unesp), Institute of Biosciences, São Paulo
dc.description.affiliationCenter for the Study of Venoms and Venomous Animals (Cevap) São Paulo State University (Unesp), São Paulo
dc.description.affiliationDivision of Biostatistics Department of Biodiversity and Biostatistics São Paulo State University (Unesp) Institute of Biosciences, São Paulo
dc.description.affiliationUnespSão Paulo State University (Unesp) Medical School, São Paulo
dc.description.affiliationUnespDivision of Anatomy Department of Structural and Functional Biology São Paulo State University (Unesp), Institute of Biosciences, São Paulo
dc.description.affiliationUnespCenter for the Study of Venoms and Venomous Animals (Cevap) São Paulo State University (Unesp), São Paulo
dc.description.affiliationUnespDivision of Biostatistics Department of Biodiversity and Biostatistics São Paulo State University (Unesp) Institute of Biosciences, São Paulo
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFAPESP: 17/06472-2
dc.description.sponsorshipIdCAPES: Finance Code 001
dc.format.extent522-536
dc.identifierhttp://dx.doi.org/10.1002/mus.27815
dc.identifier.citationMuscle and Nerve, v. 67, n. 6, p. 522-536, 2023.
dc.identifier.doi10.1002/mus.27815
dc.identifier.issn1097-4598
dc.identifier.issn0148-639X
dc.identifier.scopus2-s2.0-85150817003
dc.identifier.urihttp://hdl.handle.net/11449/248561
dc.language.isoeng
dc.relation.ispartofMuscle and Nerve
dc.sourceScopus
dc.subjectheterologous fibrin sealant
dc.subjectimmune response
dc.subjectnerve regeneration
dc.subjectneuromuscular junction
dc.subjectperipheral nerve injury
dc.titleNeuroregeneration and immune response after neurorrhaphy are improved with the use of heterologous fibrin biopolymer in addition to suture repair aloneen
dc.typeArtigo
unesp.author.orcid0000-0003-2660-6089[1]
unesp.author.orcid0000-0001-9774-1435[2]
unesp.author.orcid0000-0001-6040-7898[3]
unesp.author.orcid0000-0002-7419-3162[4]
unesp.author.orcid0000-0002-9855-5594[5]
unesp.author.orcid0000-0001-6952-0512[6]
unesp.author.orcid0000-0002-7719-9682[7]
unesp.author.orcid0000-0002-3079-4468[8]
unesp.author.orcid0000-0001-7870-5404[9]

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Botucatu - CEVAP - Centro de Estudos de Venenos e Animais Peçonhentos