High percentages of larval tetraploids in the yellowtail tetra Astyanax altiparanae induced by heat-shock: The first case in Neotropical characins

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dc.contributor.authorNascimento, Nivaldo Ferreira do [UNESP]
dc.contributor.authorPereira-Santos, Matheus [UNESP]
dc.contributor.authorLevy-Pereira, Nycolas
dc.contributor.authorMonzani, Paulo Sergio
dc.contributor.authorNiedzielski, Daiane
dc.contributor.authorFujimoto, Takafumi
dc.contributor.authorSenhorini, Jose Augusto
dc.contributor.authorOkada Nakaghi, Laura Satiko [UNESP]
dc.contributor.authorYasui, George Shigueki
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionChico Mendes Inst Biodivers Conservat
dc.contributor.institutionUniv Fed Rural Rio de Janeiro
dc.contributor.institutionHokkaido Univ
dc.date.accessioned2020-12-10T19:50:25Z
dc.date.available2020-12-10T19:50:25Z
dc.date.issued2020-04-15
dc.description.abstractTetraploid fish are an important source of diploid gametes for polyploid production, and they can be induced by inhibition of the first mitotic cell division. Dispite its potential, very few protocols regarding tetraploidization and subsequent application to mass production of triploid fish for aquaculture exist. In the yellowtail tetra Astyanax altiparanae, triploids present sterility and increased carcass yield, suggesting the generation of tetraploid for large-scale production of such triploids. In this study, the efficacy of heat shock treatments (40 degrees for 2 min) at 16, 18, 20, 22, 24, and 26 min post-fertilization (mpf) at an incubation temperature of 22 degrees C were compared on tetraploid induction of A. altiparanae. Ploidy status was confirmed by flow cytometry, karyotyping, and nuclear diameter of erythrocytes. Afterwards, sperm parameters and fertility capacity of tetraploid males were evaluated. The results showed that heat shock decreased the hatching rate, especially at 22 (19.1 +/- 9.6%), 24 (16.0 +/- 9.1%), and 26 mpf (10.3 +/- 6.2%) in comparison with the control group (62.2 +/- 7.7%). The flow cytometric analysis showed that the control group and the treatments with heat shock at 16 and 18 mpf presented only diploid individuals. However, from 20 mpf onwards, tetraploid individuals were observed, and the highest percentage arose at 26 mpf (94.55%). When mature, tetraploid males are capable of producing diploid spermatozoa with fertilization capacity, generating a 100% triploid offspring. The results indicate that the heat shock treatment at 26 mpf (40 degrees C for 2 min) is optimum for tetraploid induction in the A. altiparanae. Additionally, tetraploid mature males generated diploid spermatozoa and can be used to mass production of triploid fish. The current data are the first report of tetraploids in family Characidae, presenting an application in basic and applied sciences.en
dc.description.affiliationSao Paulo State Univ, Aquaculture Ctr, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, Brazil
dc.description.affiliationChico Mendes Inst Biodivers Conservat, Natl Ctr Res & Conservat Continental Fish, Lab Fish Biotechnol, Rodovia Pref Euberto Nemesio Pereira de Godoy, BR-13630970 Pirassununga, SP, Brazil
dc.description.affiliationUniv Fed Rural Rio de Janeiro, BR-465 Km 7, BR-23897000 Seropedica, RJ, Brazil
dc.description.affiliationHokkaido Univ, Fac Fisheries Sci, 3-1-1 Minato Cho, Hakodate, Hokkaido 0418611, Japan
dc.description.affiliationUnespSao Paulo State Univ, Aquaculture Ctr, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2010/17429-1
dc.description.sponsorshipIdFAPESP: 2011/11664-1
dc.description.sponsorshipIdFAPESP: 2016/12383-0
dc.description.sponsorshipIdCNPq: 471140/2012-0
dc.format.extent6
dc.identifierhttp://dx.doi.org/10.1016/j.aquaculture.2020.734938
dc.identifier.citationAquaculture. Amsterdam: Elsevier, v. 520, 6 p., 2020.
dc.identifier.doi10.1016/j.aquaculture.2020.734938
dc.identifier.issn0044-8486
dc.identifier.urihttp://hdl.handle.net/11449/196611
dc.identifier.wosWOS:000515498200040
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofAquaculture
dc.sourceWeb of Science
dc.subjectFish
dc.subjectChromosome manipulation
dc.subjectHeat-shock
dc.subjectPolyploidy
dc.titleHigh percentages of larval tetraploids in the yellowtail tetra Astyanax altiparanae induced by heat-shock: The first case in Neotropical characinsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.

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