Silk formation mechanisms in the larval salivary glands of Apis mellifera (Hymenoptera : Apidae)
dc.contributor.author | Silva-Zacarin, ECM | |
dc.contributor.author | De Moraes, RLMS | |
dc.contributor.author | Taboga, SR | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2014-05-20T13:55:37Z | |
dc.date.available | 2014-05-20T13:55:37Z | |
dc.date.issued | 2003-12-01 | |
dc.description.abstract | The mechanism of silk formation in Apis mellifera salivary glands, during the 5th instar, was studied. Larval salivary glands were dissected and prepared for light and polarized light microscopy, as well as for scanning and transmission electron microscopy. The results showed that silk formation starts at the middle of the 5th instar and finishes at the end of the same instar. This process begins in the distal secretory portion of the gland, going towards the proximal secretory portion; and from the periphery to the center of the gland lumen. The silk proteins are released from the secretory cells as a homogeneous substance that polymerizes in the lumen to form compact birefringent tactoids. Secondly, the water absorption from the lumen secretion, carried out by secretory and duct cells, promotes aggregation of the tactoids that form a spiral-shape filament with a zigzag pattern. This pattern is also the results of the silk compression in the gland lumen and represents a high concentration of macromolecularly well-oriented silk proteins. | en |
dc.description.affiliation | Univ Estadual Paulista, Depto Biol IB, BR-13506900 Rio Claro, SP, Brazil | |
dc.description.affiliation | IBILCE, Depto Biol, Sao Jose do Rio Preto, SP, Brazil | |
dc.description.affiliationUnesp | Univ Estadual Paulista, Depto Biol IB, BR-13506900 Rio Claro, SP, Brazil | |
dc.description.affiliationUnesp | IBILCE, Depto Biol, Sao Jose do Rio Preto, SP, Brazil | |
dc.format.extent | 753-764 | |
dc.identifier | http://dx.doi.org/10.1007/BF02708436 | |
dc.identifier.citation | Journal of Biosciences. Bangalore: Indian Academy Sciences, v. 28, n. 6, p. 753-764, 2003. | |
dc.identifier.doi | 10.1007/BF02708436 | |
dc.identifier.issn | 0250-5991 | |
dc.identifier.lattes | 1445259468526188 | |
dc.identifier.orcid | 0000-0002-0970-4288 | |
dc.identifier.uri | http://hdl.handle.net/11449/19910 | |
dc.identifier.wos | WOS:000187281000016 | |
dc.language.iso | eng | |
dc.publisher | Indian Academy Sciences | |
dc.relation.ispartof | Journal of Biosciences | |
dc.relation.ispartofjcr | 1.528 | |
dc.relation.ispartofsjr | 0,651 | |
dc.rights.accessRights | Acesso restrito | |
dc.source | Web of Science | |
dc.subject | bee | pt |
dc.subject | electron microscopy | pt |
dc.subject | larval salivary gland | pt |
dc.subject | light microscopy | pt |
dc.subject | polarized light microscopy | pt |
dc.subject | silk gland | pt |
dc.title | Silk formation mechanisms in the larval salivary glands of Apis mellifera (Hymenoptera : Apidae) | en |
dc.type | Artigo | |
dcterms.license | http://www.springer.com/open+access/authors+rights | |
dcterms.rightsHolder | Indian Academy Sciences | |
unesp.author.lattes | 1445259468526188 | |
unesp.author.orcid | 0000-0002-0970-4288[3] | |
unesp.author.orcid | 0000-0002-0970-4288[3] | |
unesp.campus | Universidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claro | pt |
unesp.campus | Universidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Preto | pt |
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