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  • ItemArtigo
    Intra-individual floral variation improves male fitness in a hummingbird-pollinated species
    (2023-05-01) Paglia, Isis; Pinto, Alessandra Ribeiro; Amorim, Felipe W. [UNESP]; Arceo-Gómez, Gerardo; Freitas, Leandro; Jardim Botânico do Rio de Janeiro. Rua Pacheco Leão; Universidade Federal do Rio de Janeiro (UFRJ); Universidade Estadual Paulista (UNESP); East Tennessee State University
    Intra-individual variation in floral traits is widespread but yet poorly understood. Variations in flower size within individuals can have implications for plant reproductive success, hence may be under pollinator-mediated phenotypic selection. Most studies on pollinator-mediated selection only evaluate female reproductive success, although floral traits can be under stronger or opposing selection via male fitness. Here we evaluated selection on intra-individual variation and integration of floral traits via its effects on male reproductive success (pollen removal) on a hummingbird-pollinated species. We sampled flowers of Justicia sebastianopolitanae that were exposed to pollinators for 24 h and counted the number of pollen grains that remained in the anthers. We measured corolla length, corolla tube length, anther, and stigma height, as well as herkogamy, display size, and estimated the coefficient of variation (CV) of traits for each individual. We found that the greatest variation in floral traits occurs within individuals. Although intra-individual variation in anther height positively affected pollen removal, we did not detect significant selection, implying that intra-individual variation is not a target for pollinator-mediated selection through male fitness in this plant population. We also found selection on the mean of anther height, indicating the importance of the spatial separation between male and female structures. The variation in anther height may increase the area of pollen deposition on the pollinator's body. Therefore, intra-individual variation could increase the chance of an individual's pollen reaching more stigmas with different heights. We highlight that intra-individual variation should be more widely studied in the context of pollinator-mediated selection and its importance for the reproductive success of individuals in a population.
  • ItemArtigo
    TNFSF13B rs9514828 gene polymorphism and soluble B cell activating factor levels: Association with apical periodontitis
    (2023-04-01) Cruz, Alvaro; Gascón, Luis Gerardo; Palafox-Sánchez, Claudia Azucena; Flores-García, Christian; Espinoza-García, Noemí; Sagrero-Fabela, Nefertari; Cintra, Luciano Tavares Angelo [UNESP]; Mejía-Flores, Rocío; Salazar-Camarena, Diana Celeste; Universidad de Guadalajara (UDG); Universidade Estadual Paulista (UNESP)
    Aim: The aim of this case-control study was to evaluate the association between the TNFSF13B rs9514828 (−871 C > T) polymorphism and soluble BAFF (sBAFF) in apical periodontitis (AP) patients. Methodology: Two hundred and sixty one healthy subjects (HS) and 158 patients with AP classified as: 46 acute apical abscess (AAA), 81 primary AP (pAP) and 31 secondary AP (sAP) patients were included. Genomic DNA (gDNA) was extracted from peripheral blood cells according to the salting out method. The TNFSF13B rs9514828 (NC_000013.11:g.108269025C > T) were identified using polymerase chain reaction (PCR) followed by restriction fragment length polymorphisms (RFLP). Serum sBAFF levels were measured by ELISA test. The chi-squared or Fisher's exact test was performed. Odds ratios (OR) and 95% confidence intervals (95% CI) were calculated to evaluate the risk of AP associated with the rs9514828. The Mann–Whitney U test and Kruskal–Wallis analysis were used for non-normally distributed data. Differences were considered significant with a p-value <.05. Results: No differences in the genotype/allele frequencies were shown between HS and patients with AAA. However, the TT genotype (OR = 2.68, 95% CI: 1.10–6.53; p =.025) and T allele (OR = 1.46, 95% CI: 1.00–2.12; p =.045) were associated with increased risk of pAP. In contrast, the minor allele T significantly decreased the risk of sAP (OR = 0.49, 95% CI: 0.024–0.99; p =.043). sBAFF serum levels were increased in AAA and pAP compared with HS (p <.01 and p =.021, respectively). The AAA patients had higher sBAFF serum levels than pAP (p =.034) and sAP (p <.01). Conclusions: These results suggest that the TNFSF13B rs9514828 (−871 C > T) polymorphism is associated with pAP susceptibility and that BAFF is a cytokine that might be involved in acute and chronic AP. The future exploration of the rs9514828 polymorphism in other AP cohorts is recommended.
  • ItemArtigo
    Influence of supplement administration of omega-3 on the subcutaneous tissue response of endodontic sealers in Wistar rats
    (2022-10-01) de Oliveira, Pedro Henrique Chaves [UNESP]; Gomes Filho, João Eduardo [UNESP]; Rodrigues, Michael Júnio da Silva [UNESP]; da Silva, Cristiane Cantiga [UNESP]; Cardoso, Carolina da Barros Moraes [UNESP]; Cosme daSilva, Leopoldo [UNESP]; Ervolino, Edilson [UNESP]; Cintra, Luciano Tavares Angelo [UNESP]; Universidade Estadual Paulista (UNESP); Alagoas Federal University (UFAL)
    Aim: Natural substances such as omega-3 have been used in the medical field due to their numerous properties and, in particular, modulating effect on the systemic and local inflammatory processes. Thus, this study evaluated the influence of omega-3 supplementation on the subcutaneous tissue response of endodontic sealers in Wistar Rats. Methodology: Polyethylene tubes were implanted in the subcutaneous tissue of 48 animals (one empty for control and three filled with Sealapex, AH Plus or Endofill). The animals were treated with omega-3 (TO) or water (TW). Treatments started 15 days before implantation until euthanasia. After 5, 15 and 30 days (n = 8), animals were euthanized and polyethylene tubes and surrounding tissue were removed and processed for histological analysis. The inflammatory reaction was analysed by Haematoxylin and Eosin stain and immunolabelling for IL-6 and TNF-α. The collagen maturity was analysed by picrosirius red stain and calcium deposition by von Kossa stain and polarized light. Results were statistically analysed (p <.05). Results: Amongst TW sealer groups, Endofill evoked a more intense inflammatory infiltrate compared with AH Plus and control in the 30-day period (p =.009). However, in TO sealer groups, there was no difference amongst the sealers and control in all periods (p >.05). Comparing each sealer as a function of the supplementation with water or omega-3, there are differences for Endofill (p =.001) and Sealapex (p =.005) in the 30-day period, presenting lower inflammatory infiltrate in the animals treated with omega-3. A higher percentage of immature fibres was observed at 15 and 30 days in the TO group, compared with the TW group (p <.05). The deposition of calcium particles was observed only by Sealapex in all periods, despite the supplementation procedure. Conclusions: Omega-3 supplementation influence the tissue reactions of endodontic sealers, modulating inflammation, the immunolabelling of IL-6 and TNF-α, the repair process and it does not interfere with calcium deposition.
  • ItemArtigo
    Interleukin-6, tumor necrosis factor-α, and CD5 immunolabeling of new experimental endodontic sealer and repair material
    (2022-01-01) Benetti, Francine [UNESP]; Ferreira, Luciana Louzada [UNESP]; Dos Reis-Prado, Alexandre Henrique; Faria, Flávio Duarte [UNESP]; Ervolino, Edilson [UNESP]; Berbert, Fabio Luiz Camargo Vellela [UNESP]; Leonardo, Renato de Toledo [UNESP]; Dias, João; Gomes-Filho, João Eduardo [UNESP]; Cintra, Luciano Tavares Angelo [UNESP]; Universidade Federal de Minas Gerais (UFMG); Universidade Estadual Paulista (UNESP); Universidade Estadual de Maringá (UEM)
    The aim of this study was to evaluate the biocompatibility and immunoinflammatory response of the Sealepox and Sealepox-RP, based on interleukin (IL)-6, tumor necrosis factor (TNF)-α, and CD5 immunolabelling. The ProRoot MTA (PRMTA) was used for comparison. Polyethylene tubes (1.0-mm internal, 1.6-mm external diameter, and 10.0-mm length; ISO 10993) with or without (control) materials were randomly implanted in the dorsum of 35 rats (4 per rat). After 7, 15, 30, 60, and 90 days (n = 7), the tubes were removed for histological and immunohistochemical analysis. The Kruskal–Wallis and Dunn’s test for non-parametric data and, ANOVA and Tukey test for parametric data were used (P < 0.05). Hematoxylin and eosin staining revealed that the concentration of inflammatory cells decreased over time with no differences between groups in all periods (P > 0.05). Regarding IL-6 immunostaining, there was no difference at 7 days (P > 0.05); all groups decreased over time, being faster for the PRMTA group and also, with no differences between groups in the last period (P > 0.05). For TNF-α, at 7 days there was no difference between groups (P > 0.05); there was an increase at 15 days for PRMTA and, at 30 and 60 days, for PRMTA and Sealepox compared to the control (P < 0.05). At 90 days, Sealepox RP showed the lowest immunostaining being similar to the control (P > 0.05). Regarding CD5 cells, at 7 days, there was high immunostaining for PRMTA compared to the control (P < 0.05); and significant reduction over time with difference for all groups at 30 and 60 days. (P < 0.05); Sealepox was similar to the control in all periods (P > 0.05). Sealepox RP showed the highest immunostaining at 15 days, being different from the control and PRMTA (P < 0.05); in the other periods it was similar to the control (P > 0.05). It can be concluded that Sealepox and Sealepox-RP were biocompatible and demonstrated similar immunoinflammatory response regarding IL-6, TNF-α, and CD5 compared to PRMTA.
  • ItemArtigo
    Curcumin reduces inflammation in rat apical periodontitis
    (Wiley-Blackwell, 2022-09-05) Justo, Mariana Pagliusi [UNESP]; Cardoso, Carolina de Barros Morais [UNESP]; Cantiga-Silva, Cristiane [UNESP]; Oliveira, Pedro Henrique Chaves de [UNESP]; Sivieri-Araujo, Gustavo [UNESP]; Azuma, Mariane Maffei [UNESP]; Ervolino, Edilson [UNESP]; Cintra, Luciano Tavares Angelo [UNESP]; Universidade Estadual Paulista (UNESP)
    Aim The aim of the study was to evaluate the effect of systemic curcumin administration on the severity of apical periodontitis (AP). Methodology Forty male Wistar rats weighing 250-280 g each, age 2.5 months, were distributed into four groups (n = 10): control untreated rats (C), control rats treated with curcumin (CUR), rats with pulp exposure-induced apical periodontitis (AP) and rats with pulp exposure-induced apical periodontitis treated with curcumin (AP-CUR). Curcumin treatment was administered orally once daily for 15 days before pulp exposure and continued for 30 days after pulp exposure. The rats were sacrificed at 30 days, and the jaws were collected and reconstructed in a programme specific for micro-CT. The jaws were processed for analysis of the inflammatory process using haematoxylin and eosin staining and immunohistochemical assays for interleukin tumour necrosis factor alpha (TNF-alpha), interleukin (Il)-6 and Il-1 beta. Tartrate-resistant acid phosphatase (TRAP) and osteocalcin (OCN) staining were used to analyse the resorptive process on the bone surface of periapical area. Kruskal-Wallis with Dunn's test was performed for nonparametric data and anova with Tukey's test for parametric data, p < .05. Results Micro-CT revealed no statistically significant differences in bone resorption between the AP and AP-CUR groups (p > .05). The levels of inflammatory cell infiltration and immunoreactivity for the proinflammatory cytokines TNF-alpha, Il-6 and Il-1 beta were significantly higher in the periapical lesions of the AP group than in the AP-CUR group (p < .05). The number of TRAP-positive multinucleated cells was higher in the AP group than in the AP-CUR group (p < .05). In OCN-positive cells, no differences were observed between the AP and AP-CUR groups (p > .05). Conclusions Oral supplementation with curcumin had a significant effect on the AP severity in rats, suggesting an anti-inflammatory effect of curcumin on AP development.
  • ItemArtigo
    A universal pharmacy: Possible self-medication using tree balsam by multiple Atlantic Forest mammals
    (2022-01-01) Kaisin, Olivier [UNESP]; Rocha, Fernanda Corrêa; Amaral, Rodrigo Gonçalves [UNESP]; Bufalo, Felipe [UNESP]; Sabino, Gabriel Pavan [UNESP]; Culot, Laurence [UNESP]; University of Liège (Uliège); Universidade Estadual Paulista (UNESP); University of Brasília (UnB)
    We present camera trap evidence of 10 Atlantic Forest mammals fur-rubbing, licking, or biting balsam from cabreúvas (Myroxylon peruiferum, Fabaceae), native trees used in traditional medicine for their prophylactic and therapeutic virtues. Given the antiparasitic properties of cabreúvas, mammals may be using the balsam as topical self-medication to repel ectoparasites. Abstract in Portuguese is available with online material.
  • ItemArtigo
    Method 300: a complementary teaching methodology in Dentistry
    (2022-04-01) Rodrigues, Gladiston Willian Lobo [UNESP]; de Souza Gomide Guimarães, Maria Rosa Felix; Guimarães, Gustav; Rosa, Flávia Gabriela; Samuel, Renata Oliveira; Cantiga-Silva, Cristiane [UNESP]; de Barros Morais Cardoso, Carolina [UNESP]; Cintra, Luciano Tavares Angelo [UNESP]; University Center São Lucas; Universidade Estadual Paulista (UNESP); University of Uberaba
    This study evaluated Method 300, a complementary teaching methodology in Dentistry. Thirty participants were divided into 6 groups according to the scores obtained in a placement test ranging from 0 to 10. Students with scores lower than 6 were classified as having poor academic performance; students with scores equal to or greater than 6 were considered as having good academic performance. Each group included at least one student with good performance. Our methodology consisted of two application cycles, each including one different test; however, only students with low performance could undergo the second testing. Students held meetings twice a week for one hour and thirty minutes. Of the 54 students initially enrolled in the subject, 24 dropped out, leaving 30 students who completed all the proposed activities. In cycle 1, 24 (80%) students showed poor academic performance and 6 (20%) good. Students with poor performance in P1 significantly improved after P1 300 test application (P < 0.001). After cycle 1 activities, all 30 participants showed significant improvement. In cycle 2, the number of students with good performance tripled when compared to cycle 1, resulting in a decrease in the number of students with poor performance (P = 0.205). Results show that the Method 300 is an important teaching–learning resource in Dentistry, to be used as a complementary methodology to lectures.
  • ItemArtigo
    Accuracy of radiographic pixel linear analysis in detecting bone loss in periodontal disease: Study in diabetic rats
    (2021-01-01) Bueno, Carlos Roberto Emerenciano [UNESP]; Sumida, Doris Hissako [UNESP]; Duarte, Marco Antônio Hungaro; Ordinola-Zapata, Ronald; Azuma, Mariane Maffei [UNESP]; Guimarães, Gustav; Pinheiro, Tiago Novaes; Cintra, Luciano Tavares Angelo [UNESP]; Universidade Estadual Paulista (UNESP); Universidade de São Paulo (USP); School of Dentistry; University of Michigan; Dental School of Amazonas State University
    Introduction: Periodontitis, a complex infectious disease that may lead to irreversible loss of periodontium, is considered a predisposing agent for developing insulin resistance due to the release of inflammatory mediators, showing a bilateral relationship with diabetes mellitus. The investigation of periodontal disease requires a clinical approach and complete intraoral radiographs, even with increasing concerns about radiation exposure. Thus, this study assesses pixel linear analysis accuracy using digital radiography via Digora® in detecting alveolar bone destruction in diabetic rats with periodontal disease. Methodology: 40 rats were divided into groups (n = 10): control (C), rats with periodontal disease (PD), experimental diabetic rats (ED), experimental diabetic rats with periodontal disease (ED-PD). Diabetes was induced by streptozotocin and periodontal disease by periodontal ligature. After 30 days, maxillae bone destruction was obtained by linear analysis of vertical bone loss using digital radiography and then assessed by micro-CT and histology. Data were analyzed by ANOVA and Tukey's test (p < 0.05). Results: Radiographic, micro-CT and histological analysis presented accurate and similar results. PD and ED-PD groups showed higher bone destruction than C and ED groups (p < 0.05). Moreover, the ED-PD group had higher bone loss than the PD group (p < 0.05). Conclusion: The pixel linear analysis via digital radiography was an accurate, low-cost alternative in detecting alveolar bone loss in this rat model. Micro-CT and histological analysis may also be used to obtain linear measures to assess and compare periodontal bone destruction in diabetic rats.
  • ItemResenha
    Evolution of endodontic medicine: a critical narrative review of the interrelationship between endodontics and systemic pathological conditions
    (2021-10-01) Cintra, Luciano Tavares Angelo [UNESP]; Gomes, Maximiliano Schünke; da Silva, Cristiane Cantiga [UNESP]; Faria, Flávio Duarte [UNESP]; Benetti, Francine; Cosme-Silva, Leopoldo; Samuel, Renata Oliveira; Pinheiro, Tiago Novaes; Estrela, Carlos; González, Alvaro Cruz; Segura-Egea, Juan José; Universidade Estadual Paulista (UNESP); Pontifical Catholic University of Rio Grande do Sul; Universidade Federal de Minas Gerais (UFMG); Federal University of Alfenas (Unifal); Universidade de Uberaba; Dental School of Amazonas State University; Universidade Federal de Goiás (UFG); University of Guadalajara; University of Sevilla
    Endodontics has gained emphasis in the scientific community in recent years due to the increase in clinical and in animal models studies focused on endodontic medicine, which aims to evaluate the interrelationship between systemic and periapical tissues pathological conditions. These studies have shown that systemic changes can boost the pathogenesis of endodontic infection, favoring its development and progression. A contrary relationship is reported in numerous studies that affirm the potential of endodontic infection to trigger systemic damage and may lead to the worsening of pre-existing pathologies. Recently, the potential of filling materials to develop systemic changes such as neurological alterations had been evaluated, also showing that systemic diseases can negatively influence tissue responses to filling materials after endodontic treatment. Despite advances in endodontic medicine studies, there are still gaps in knowledge on the mechanisms of interactions between apical periodontitis (AP) and systemic diseases and much research to be done. In this sense, this critical narrative literature review aimed to show the evolution of studies in endodontic medicine to help the endodontist to know the role of systemic diseases in the pathogenesis of AP and the possible interference in the repair of periapical tissues after endodontic treatment, as well as to evidence the systemic complications that can be triggered or aggravated in the presence of endodontic infection.
  • ItemArtigo
    Inflammatory profile of apical periodontitis associated with liver fibrosis in rats: histological and immunohistochemical analysis
    (2021-01-01) Cantiga-Silva, C. [UNESP]; Estrela, C.; Segura-Egea, J. J.; Azevedo, J. P. [UNESP]; de Oliveira, P. H.C. [UNESP]; Cardoso, C. B.M. [UNESP]; Pinheiro, T. N.; Ervolino, E. [UNESP]; Sivieri-Araújo, G. [UNESP]; Cintra, L. T.A. [UNESP]; Universidade Estadual Paulista (Unesp); Universidade Federal de Goiás (UFG); University of Sevilla; Dental School of Amazonas State University
    Aim: To investigate the effects of liver fibrosis (LF) on the pro-inflammatory mediators and periapical bone resorption of apical periodontitis (AP) in rats. Methodology: Forty male Wistar rats were distributed into four groups: C – control, AP – rats with AP, LF – rats with LF, AP + LF – rats with AP and LF. LF was induced by carbon tetrachloride administration for 8 weeks and surgical bile duct ligation for 4 weeks; AP was induced in the teeth of rats by dental pulp exposure to the oral environment for 30 days. Jaws and livers were removed after euthanasia. Haematoxylin and Eosin (H&E) and Picrosirius Red (PSR) staining were used to confirm fibrosis in the livers. The jaws were analysed using H&E staining, immunohistochemical assays of interleukin (IL)-1β, IL-6 and tumour necrosis factor-alpha (TNF-α). Student’s t-test and Mann–Whitney’s U-test were used for statistical analysis (P < 0.05). Results: Inflammatory infiltrate was moderate in the AP group and severe in the AP + LF group (P < 0.05). Periapical bone resorption was significantly larger in the AP + LF group compared with the AP group (P < 0.05). IL-1β, IL-6 and TNF-α levels were significantly higher in AP + LF group when compared to the AP group (P < 0.05). Conclusion: More intense inflammatory infiltrate, greater amounts of pro-inflammatory cytokines and increased periapical bone resorption were observed in the presence of liver fibrosis in rats with exposed pulps.
  • ItemResenha
    Prevalence of gingival hyperplasia induced by anticonvulsants: A systematic review
    (2021-01-01) Cláudio, Marina Módolo [UNESP]; Rodrigues, João Victor Soares [UNESP]; Garcia, Valdir Gouveia; Theodoro, Leticia Helena [UNESP]; Universidade Estadual Paulista (Unesp); Latin American Institute of Dental Research and Education (ILAPEO)
    Objective: Gingival hyperplasia (GH) is one of the side effects of anticonvulsant drugs. The aim of this study was to verify the prevalence of GH associated with the use of anticonvulsant, through a systematic review. Material and Methods: Systematic search was done at databases Pubmed and Embase between January 1984 and March of 2020 for identification of articles addressing the prevalence of GH associated with the use of anticonvulsant drugs. The methodological index for non-randomized studies (MINORS) was independently assessed for quality in the selected papers. Results: The search identified 4.471 references. Nine articles were selected and evaluated 632 participants. All of the studies included in the systematic review showed a low risk of bias. The anticonvulsants used by patients were carbamazepine, ethosuximide, phenytoin, primidone, phenobarbital, sodium valproate. The studies showed a correlation between different types of anticonvulsants and GH prevalence, with a range from 0% to 73%. Among the anticonvulsants used, phenytoin showed the greatest incidence of GH, varying between 15.61% and 73% in patients. Conclusion: In the analysis of the results obtained in the literature, it is possible to notice that the great majority of studies presented incidence of GH associated with anticonvulsant use. However, further studies are necessary to understand the anticonvulsant action mechanism inducing GH, as well as the prevention forms, given that GH is a significant side effect.
  • ItemResenha
    The use of omega-3 fatty acids in the treatment of oral diseases
    (2020-01-01) Azuma, Mariane Maffei; Cardoso, Carolina de Barros Morais [UNESP]; da Silva, Cristiane Cantiga [UNESP]; de Oliveira, Pedro Henrique Chaves [UNESP]; Jacinto, Rogério de Castilho [UNESP]; Andrada, Ana Cristina; Cintra, Luciano Tavares Angelo [UNESP]; University of Michigan; Universidade Estadual Paulista (Unesp); University of Detroit Mercy School of Dentistry
    Omega-3 polyunsaturated fatty acids (ω-3 PUFAs) have been reported to exert important roles in the inflammatory response. There are many inflammatory diseases in dentistry which support the administration of ω-3 PUFAs as an adjunct therapy during the treatment of these diseases. The aim of this review was to evaluate the use of ω-3 PUFAs as an adjuvant therapy during the treatment of buccal diseases. The review showed that supplementation with ω-3 PUFAs was used for treatment of gingivitis, periodontal diseases, apical periodontitis, stomatitis, and orthodontic tooth movement. The results indicate that ω-3 PUFAs decreased the number of pro-inflammatory mediators in the gingival tissues of individuals with gingivitis and periodontitis. In apical periodontitis, the supplementation suppressed bone resorption and promoted bone formation in the periapical area of rats. During orthodontic movement, the supplementation showed a decrease of bone resorption in rats. It also showed that painful symptoms of recurrent aphthous stomatitis were alleviated in supplemented patients. In conclusion, the ω-3 PUFAs may be used as an adjuvant therapy in the treatment of inflammatory diseases that affect the oral cavity. However, more studies are required to elucidate the role of ω-3 PUFAs in decreasing oral cavity inflammatory processes.
  • ItemArtigo
    Prevalence of Cryptosporidium infection in domestic cats from an urban area in Brazil
    (2020-12-01) da Silveira Neto, Luiz; Widmer, Giovanni; de Lima, Valéria Marçal Felix [UNESP]; Meireles, Marcelo Vasconcelos [UNESP]; Inácio, Sandra Valéria [UNESP]; Marques, Marcel Gambin; Marques, Ana Elisa Gregui Watanabe [UNESP]; de Matos, Lucas Vinícius Shigaki; da Cruz Panegossi, Mariele Fernanda; Bresciani, Katia Denise Saraiva [UNESP]; UFT; Tufts University; Universidade Estadual Paulista (Unesp); UNIFIO; Ipanema Indústria de Produtos Veterinários; Centro Universitário Max Planck
    We investigated the occurrence of Cryptosporidium oocysts shedding by domestic cats in an urban setting. The calculation of minimum sample size was based on an estimated prevalence of 10%, 5% absolute sampling error and a 5% significance level, resulting in 138 cats. A total of 612 owners of 2,290 cats had to be contacted for achieving the minimal sample size. In the end, only 55 owners accepted to participate in this investigation. Stool samples collected from 138 dogs were examined by microscopy using modified Kinyoun acid-fast staining, capture ELISA and nested-PCR followed by sequencing. Samples were considered positive when Cryptosporidium were detected by at least two diagnostic methods. Thirteen samples were positive (9.4%; 95% CI: 4.5 - 14.3). Cryptosporidium amplicons from seven out of the 13 samples were successfully sequenced and shared 99% genetic similarity to Cryptosporidium felis, GenBank access AF112575.1 was found. We concluded that Cryptosporidium infection is common in domestic cats from urban area and veterinary practitioners should guide cat owners to adopt preventive measures against the parasite to reduce the chance of infection in cats and householders.
  • ItemArtigo
    Climbing plants of a semideciduous seasonal forest remnant in southeastern Brazil
    (2019-01-01) Scudeler, Ana Laura; Castello, Ana Carolina Devides [UNESP]; Rezende, Andréia Alves [UNESP]; Koch, Ingrid [UNESP]; Universidade Estadual de Campinas (UNICAMP); Universidade Estadual Paulista (Unesp)
    Climbing plants play an important role on the dynamics of forest communities and are important as a source of food for wildlife. In this study we characterized these plants in a semideciduous seasonal forest (FES) and produce identification key and photographic plates to help in the identification of the climbing plants. In addition, we evaluate the floristic similarity of the study area with studies in other areas. The floristic survey was carried out during one year, with monthly collections of material at the reproductive stage, through walks at the edges and inside the remnant. We sampled 65 species belonging to 19 families, and six represent 67.7% of the total species sampled: Fabaceae (12), Bignoniaceae (eight), Convolvulaceae (seven), Sapindaceae (six), Asteraceae (six) and Apocynaceae (five). There was predominance of herbaceous climbers, on the edge of the remnant or in the open areas. The most common climbing mechanisms were the voluble and the prehensile (with tendrils). The low similarity found with other areas of FES may be related to small size of the study area and its intermediate succession stage. Nevertheless, 64% of the species are new records for the municipality of Sorocaba, many of them with few records for the state of São Paulo and two of them classified as threatened in the list of threatened species of the state.
  • ItemArtigo
    Soluble yerba mate (Ilex Paraguariensis) extract enhances in vitro osteoblastic differentiation of bone marrow-derived mesenchymal stromal cells
    (2019-11-15) Balera Brito, Victor Gustavo; Chaves-Neto, Antonio Hernandes; Landim de Barros, Thamine; Penha Oliveira, Sandra Helena; Universidade Estadual Paulista (Unesp)
    ETHNOPHARMACOLOGICAL RELEVANCE: Yerba mate (Ilex paraguariensis) consumption has been associated with beneficial effects on bone health. AIM OF THE STUDY: The purpose of this study was to evaluate the mechanism by which soluble yerba mate (SYM) stimulates osteoblast differentiation of bone marrow-derived mesenchymal stromal cells (BM-MSCs). MATERIALS AND METHODS: BM-MSCs from male Wistar rats were induced towards osteoblastic differentiation with different concentrations of SYM (10, 20, and 50 μg/mL). Osteoblastic differentiation was evaluated by measuring proliferation rates, alkaline phosphatase activity, MMP-2 activity, mineralization, and gene expression of Runx2, Osterix, β-catenin (Catnb), collagen type I (Col1a1), osteopontin (Opn), osteocalcin (Ocn), bone sialoprotein (Bsp), bone morphogenetic protein-2 (Bmp2), osteoprotegerin (Opg), and Rankl. We also analyzed cytokine production and MAP kinase pathways. RESULTS: SYM (10 μg/mL) did not show a cytotoxic effect and induced a slight increase in ALP activity; however, a great increase in mineralization was observed. SYM was also able to reduce TNF-α and IL-10 production; increase the expression of transcription factors Runx2, Osterix, and Catnb; and increase matrix proteins Opn, Bsp, Ocn, and Bmp2. We also observed a decrease in intracellular signaling of ERK, JNK, and p38 MAPK, which seemed to be related to the SYM response. CONCLUSIONS: Together, these results help to explain the promoting effect on osteoblast differentiation produced by a low SYM concentration. However, a higher SYM concentration presented deleterious effects, including cytotoxicity, decreased ALP activity, increased cytokine production, decreased bone marker gene expression, increased MAPK signaling, and significant mineralization reduction. In conclusion, our results suggest a concentration-specific direct stimulatory effect of SYM on osteoblastic differentiation in vitro.
  • ItemArtigo
    Prevalence of dental anomalies in permanent dentition of Brazilian individuals with down syndrome
    (2016-08-01) Cuogh, Osmar Aparecido [UNESP]; Topolski, Francielle [UNESP]; de Faria, Lorraine Perciliano [UNESP]; Occhiena, Carla Machado [UNESP]; Pinto Ferreira, Nancy Dos Santos [UNESP]; Ferlin, Camila Ribeiro [UNESP]; de Mendonça, Marcos Rogério [UNESP]; Universidade Estadual Paulista (Unesp)
    Objective: The aim of this study was to evaluate the incidence of dental anomalies in the permanent dentition of individuals with Down Syndrome (DS) to increase the knowledge on the dental issues in this syndrome. Method: One hundred and five panoramic X-rays of patients with DS (61 males and 44 females), aged 7 to 42 years were used. The data were statistically analyzed using bivariate analyses test (p <0.05). Results: Dental anomalies were observed in 50.47% of the sample. More than one anomaly was observed in 9.52% of the individuals. The most frequent dental anomalies were hypodontia and microdontia (16.19%), followed by retained tooth (10.47%), taurodontism (9.52%), supernumerary teeth (5.71%), macrodontia (2.85%) and root dilaceration (0.95%). There was no statistically significant difference between genders for any of the anomalies. Conclusion: A high prevalence of dental anomalies was observed in individuals with DS. The results of the present study reinforce the importance of good dental care, offering a greater basis for professionals who provide dental service to these patients.
  • ItemResumo
    Centro de Assistência Odontológica a Excepcionais - CAOE
    (Universidade Estadual Paulista (Unesp), 2001) Callestini, Edmur Aparecido [UNESP]; Universidade Estadual Paulista (Unesp)
    O objetivo maior do CAOE é garantir a maximização do equilíbrio biopsicossocial de deficientes mentais, portadores ou não de outras deficiências, e para isto desenvolve suas atividades estruturado em uma sólida equipe "técnica" multidisciplinar. Esta equipe trabalha integrada diretamente à equipe odontológica, apoiando, individualizando e complementando a assistência. Afora este, são objetivos não menos importantes: 1. orientar os familiares dos excepcionais relativamente às suas interrelações pessoais e aos seus problemas de saúde, higiene, habitação e hábitos; 2. orientar alunos, especialistas, pós-graduandos, docentes e a comunidade científica para a prevenção, promoção da saúde e tratamento de pacientes especiais; 3. esclarecer a comunidade leiga sobre os esforços para integrar o paciente especial em seu meio; 4. promover, socialmente, o excepcional através de reuniões de congrassamento entre os vários segmentos da comunidade, envolvendo seus familiares. O Centro foi criado através da Resolução UNESP no. 45 de 10 de setembro de 1.984, tendo iniciado suas atividades em julho de 1.985. Atualmente, assiste mais de 4.500 pacientes procedentes de 280 cidades da Federação. Os profissionais contratados envolvidos são: Supervisor, Vice-Supervisora, Supervisora de Seção de Administração, Analista de Informática, Oficial Administrativo, Recepcionista, Secretária, Programador, Técnico em Aparelho de Precisão, Clínico Geral, Cardiologista, Neurologista, Anestesiologista, Cirurgiões Dentistas, Assistente Social, Psicóloga, Fisioterapeuta, Fonoaudiólogas, Terapeuta Ocupacional, Enfermeira, Auxiliares de Enfermagem, Auxiliares Hospitalares e Auxiliares de Odontologia. Contamos com voluntários nas seguintes especialidades: Cirurgiões Dentistas, Ortopedistas, Pediatra, Biofísico, Otorrinolaringologista e Cirurgião Plástico. Duas funcionárias que realizam a limpeza do Centro, foram contratadas por uma empresa privada, como doação para o Centro e, dois auxiliares de serviços gerais são contratados pela Sociedade Pró-Estudo, Pesquisa e Assistência de Pacientes Especiais - SEPAE, que apoia os trabalhos do Centro. Uma das primeiras medidas adotadas no Centro foi a de informatizar cerca de 400 (quatrocentas) informações de cada um dos pacientes assistidos, visando suas posteriores utilizações em atividades de divulgação leiga e científica. Convém lembrar que, com esse objetivo, o Centro foi um dos primeiros setores da Universidade e o primeiro do país a implantar seu setor de informática com esse objetivo. A filosofia de trabalho relativa às assistências foi a de que os pacientes não deveriam ser "assistidos só por assistí-los", mas assistidos objetivando sua divulgação falada e escrita, a melhoria de sua saúde bucal e geral, da qualidade de vida, e o resgate da cidadania e de seus direitos. Esta filosofia foi reforçada, dia a dia, quando pacientes oriundos de cidades a mais de 1.000 a 2.000 quilômetros do Centro, delas se deslocavam para serem ali assistidos, tornando evidente, de forma gritante, a total marginalização dos portadores de deficiências no campo da assistência médico-odontológica a esses pacientes. A evidência desta situação, levou os técnicos (Cirurgiões Dentistas, Médicos e Profissionais da saúde) do Centro a realizar conferências, palestras, seminários, mesas redondas, simpósios, sobre portadores de deficiências em todo o país. Softwares foram criados pelo Núcleo de Processamento Dedicado do Centro (NPDD) para apoio clínico, bibliográfico e às pesquisas no Centro. A consistência dos dados informatizados permitiram que fossem realizados e orientados pelo Centro, trabalhos de divulgação, de Mestrado, Doutorado e para concurso de Professor Titular. A divulgação dos trabalhos sobre pacientes especiais foi tal que a Coordenadoria de Saúde Bucal do Ministério da Saúde solicitou ao Supervisor do Centro, a preparação de Programa a ser instituído no país para a capacitação de Cirurgiões Dentistas e profissionais de áreas afins na Assistência aos Pacientes Especiais. Em Brasília, o Programa Nacional de Assistência Odontológica Integral ao Paciente Especial foi apresentado e aprovado por representantes dos vários Estados da Federação. Na oportunidade, o CAOE foi indicado como Núcleo de Capacitação no país dos profissionais da Saúde. Paralelamente, Professores Universitários inscreveram-se como alunos do Programa no Curso de Extensão Universitária, em virtude do CAOE, já tido como Centro de Excelência na Assistência e Pesquisa Clínica sobre Pacientes Especiais no país, oferecer as melhores e únicas condições para tal. Os capacitados no CAOE, através do Programa citado, multiplicaram-se pelo país e sentiram a necessidade de implantar pólos de assistência multiprofissional aos Pacientes Especiais. Um dos recursos para apoiá-los foi o de estender a ação do CAOE através de suas Jornadas Brasileiras de Estudos sobre Pacientes Especiais. Seus objetivos têm sido o de alcançar os Governadores, Prefeitos, Deputados, Vereadores, Secretários de Estado e Municípios, além dos profissionais não engajados na problemática da assistência aos Pacientes Especiais. Inúmeros Pólos, Centros e Serviços de atenção a Pacientes Especiais foram, assim, implantados em cidades do país segundo orientações do CAOE. Algumas Disciplinas sobre Pacientes Especiais foram criadas pelos Profissionais, capacitados no Centro, em Faculdades do país. Em 1997, a Faculdade de Odontologia de Araçatuba criou e implantou a primeira disciplina na UNESP sobre "Assistência Odontológica Integrada a Pacientes Especiais", ministrada no último semestre do quarto ano. Neste Curso, os técnicos do Centro, sob a Coordenação do Supervisor, ministraram aulas teóricas e práticas. Não se pode esquecer que os Cursos de Pós-Graduação de Estomatologia e de Odontopediatria em Níveis de Mestrado e Doutorado desta Faculdade, contam com a presença do Centro em suas Disciplinas. Os profissionais do CAOE foram e estão contratados como técnicos, a maioria em tempo parcial, a ser dedicado, exclusivamente, à assistência de seus pacientes. Dessa forma e pelo fato de não serem docentes, não poderiam atuar como docentes e desenvolver as funções inerentes a estes, de ensino e pesquisa. Mas desde 1.986, ou seja, seis meses após o início das atividades deste Centro, a maioria dos profissionais, devido à procura e a necessidade de assistência integral do grande número de pacientes, estão embuidos no desenvolvimento de orientações e esclarecimentos a profissionais, familiares e responsáveis pelos pacientes, através da realização de palestras, conferências, simpósios, seminários, jornadas e programas sobre pacientes especiais, no Centro e por todo o país. Orientam estagiários, alunos e profissionais, coordenam o trabalho de profissionais voluntários, além de ministrarem aulas nos Cursos de Capacitação promovidos pelo Centro, no Curso de Graduação e Pós-Graduação, citados anteriormente. Seus trabalhos apoiam-se nos 400 dados contidos nos prontuários de cada paciente e documentação audio-visual. De sua criação a nossos dias, as assistências tem sido realizadas em elevado nível científico, grangeando o apoio e respeito da classe médico-odontológica e de instituições nacionais e internacionais. Pode ser citado como testemunho deste nível o "Reconocimiento del Instituto Interamericano del Niño (Organização dos Estados Americanos) e Beach Center on Families and Disability (USA), através do prêmio Ëloisa Garcia Etchegoihen de Lorenzo, en San José da Costa Rica, em 1.993, no confronto dos valores didáticos, científicos, assistenciais e filosóficos de 41 instituições de 14 países do continente americano. Os resultados alcançados nesses catorze anos de existência do CAOE abrangem, benefícios relacionados diretamente à comunidade, através da assistência integral contínua de seus pacientes, todos seus familiares ou responsáveis que tem sido amparados pelo Centro ao receberem orientações e esclarecimentos nos vários campos da saúde, relacionamentos familiares e recebimento de auxílios auxílios, através do trabalho sócio-educativo, realizado pelos profissionais, visando maior integração junto à Sociedade e a melhoria da qualidade de vida. Além de ser um Centro de Excelência na Universidade e no país, disponibilizando um dos maiores bancos de dados sobre excepcionalidade, integra alunos e profissionais desta e de outras unidades da UNESP e de outras instituições públicas e privadas através de convênios técnicos - científicos.
  • ItemArtigo
    Omentalização prostática em cãse
    (2006-12-01) Apparício, Maricy [UNESP]; Vicente, Wilter Ricardo Russiano [UNESP]; Pirez, Eliandra Antônia [UNESP]; Mostachio, Giuliano Queiroz [UNESP]; Ribeiro, Ana Paula Coelho [UNESP]; Covizzi, Gabriela Jayme [UNESP]; Gadelha, Carla Renata Figueiredo [UNESP]; Carvalho, Marileda Bonafim [UNESP]; Universidade Estadual Paulista (Unesp)
    The prostate is the only acessory sex gland in the male dog and, although the prostate is found in all mammals, it has a great importance in men and dogs due to the frequency of disorders. Several techniques have been employed for the treatment of prostatic cysts and abscesses and, a few years ago, it was successfully described the use of prostatic omentalisation for this purpose; until now, there are no data about this surgery in Brazil. So, the aim of this study was to evaluate the animal recovery, as well as the post operative period of dogs that underwent prostatic omentalisation, from 2002 to 2004. It was evaluated 11 dogs with prostatic cysts, 4 dogs with prostatic abscesses and 2 with paraprostatic cysts. Fifteen dogs had an uneventful recovery while one dog had a minor incontinence for two days. One dog died due to a previous long term sepsis. The low incidence of post operative complications and brief hospitalization period make omentalisation the surgery of choice for the treatment of prostatic cysts and abscess.
  • ItemArtigo
    Landau and Kolmogoroff type polynomial inequalities II
    (2004-06-01) De Andrade, Eliana X.L. [UNESP]; Dimitrov, Dimitar K. [UNESP]; De Sousa, Lisandra E. [UNESP]; Universidade Estadual Paulista (Unesp)
    Let 0 < j < m ≤ n. Kolmogoroff type inequalities of the form ∥f(j)∥2 ≤ A∥f(m)∥ 2 + B∥f∥2 which hold for algebraic polynomials of degree n are established. Here the norm is defined by ∫ f2(x)dμ(x), where dμ(x) is any distribution associated with the Jacobi, Laguerre or Bessel orthogonal polynomials. In particular we characterize completely the positive constants A and B, for which the Landau weighted polynomial inequalities ∥f′∥ 2 ≤ A∥f″∥2 + B∥f∥ 2 hold. © Dynamic Publishers, Inc.
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    Salivary alpha amylase and cortisol levels in children with global developmental delay and their relation with the expectation of dental care and behavior during the intervention
    (Pergamon-Elsevier B.V. Ltd, 2012-03-01) Santos, Márcio José Possari dos [UNESP]; Bernabé, Daniel Galera [UNESP]; de Melo Stevanato Nakamune, Ana Claudia [UNESP]; Perri, Silvia Helena Venturoli [UNESP]; Coelho Avila de Aguiar, Sandra Maria Herondina [UNESP]; Oliveira, Sandra Helena Penha de [UNESP]; Universidade Estadual Paulista (Unesp)
    The purpose of this study was to analyze the alpha-amylase (sAA) and cortisol levels in children with Global developmental delay (GDD) before and after dental treatment and its association with the children's behavior during treatment. The morning salivary cortisol levels and activity of sAA of 33 children with GDD were evaluated before and after dental treatment and were compared to 19 healthy children. The behavior of children with GDD during dental care was assessed by the Frankl scale. Children with GDD showed lower levels of sAA activity than healthy children, but this result was not significant. The salivary cortisol levels were similar between GOD and healthy children. GDD children showed increased levels of sAA (but not cortisol) prior to the dental treatment as compared to the post-treatment phase. GOD children who showed less favorable behavior during dental care had higher levels of sAA and salivary cortisol than GOD children with more favorable behavior, but only the sAA results were significant. In conclusion, GOD children show hyperactivity of the SNS-axis in anticipation of dental treatment which indicates the need for strategies to reduce their anxiety levels before and during dental care. (C) 2011 Elsevier Ltd. All rights reserved.