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  • ItemArtigo
    Electrospun polylactic acid scaffolds with strontium- and cobalt-doped bioglass for potential use in bone tissue engineering applications
    (2022-01-01) de Souza, Joyce Rodrigues ; Kukulka, Elisa Camargo ; Araújo, Juliani Caroline Ribeiro ; Campos, Tiago Moreira Bastos ; do Prado, Renata Falchete ; de Vasconcellos, Luana Marotta Reis ; Thin, Gilmar Patrocínio ; Borges, Alexandre Luiz Souto ; Universidade Estadual Paulista (UNESP) ; Praça Marechal Eduardo Gomes
    The development of nanoscale biomaterials associated with polymers has been growing over the years, due to their important structural characteristics for applications in biological systems. The present study aimed to produce and test polymeric scaffolds composed of polylactic acid (PLA) fibers associated with a 58S bioglass doped with therapeutic ions for use in tissue engineering. Three 58S Bioglass was obtained by the sol–gel route, pure and doped with 5% strontium and cobalt ions. Solutions of 7% PLA was used as control and added the three different bioglass, 4% of 58S bioglass (PLA-BG), 4% bioglass-doped strontium (PLA-BGSr) and 4% bioglass-doped cobalt (PLA-BGCo). Scaffolds were produced through electrospinning process, and was characterized chemical and morphologically. The in vitro tests were performed using mesenchymal cells cultures from femurs of nine rats, grown in osteogenic supplemented total culture medium. After osteoblastic differentiation induction cell viability, alkaline phosphatase activity, total protein content quantification, and visualization of mineralization nodule tests were performed. Analysis of normal distribution used the Shapiro–Wilk test (nanofibers diameter and biological assay). Data were compared using the Kruskal–Wallis nonparametric test (p = 0.05). The bioglasses produced proved to be free of nitrate, chlorinated and nano-sized, with effective incorporation of therapeutic ions in their structure. All materials showed cell viability (>70%), total protein production, and alkaline phosphatase activity. It was possible to develop polylactic acid scaffolds associated with 58S bioglass doped with therapeutic ions without cytotoxicity. Scaffolds characteristics appear to sustain its application in bone tissue engineering.
  • ItemArtigo
    Single implant placement in the maxillary aesthetic area with or without connective tissue grafting: A 1-year follow-up randomised clinical trial
    (Quintessence Publishing Co Inc, 2022-03-01) Lazzari, Thiago Rodrigues ; Jardini, Maria Aparecida Neves ; Santos, Nidia Castro dos ; Neves, Felipe Lucas da Silva ; Lima, Victoria Clara da Silva ; Melo Filho, Antonio Braulino de ; Santamaria, Mauro Pedrine ; Universidade Estadual Paulista (UNESP) ; Univ Guarulhos
    Purpose: To evaluate the influence of connective tissue graft on the soft tissue thickness and aesthetics around single implants placed in the aesthetic zone of the maxilla. Materials and methods: Forty-two patients with indications for single implant placement in the aesthetic zone were randomly allocated into two groups: the implant group (implant insertion) and the implant + connective tissue graft group (implant insertion and placement of a 1.50-mm-thick connective tissue graft). Clinical evaluations were performed at baseline, 4 months after surgery (prior to prosthetic reconstruction) and 1 year after crown placement to assess tissue thickness at the crestal aspect and the buccal aspect, buccal defects, keratinised tissue width and proximal bone resorption. Aesthetics were assessed using the pink aesthetic score index, and postoperative discomfort and pain tests were also performed. Results: Only the implant + connective tissue graft group presented a significant increase in tissue thickness at the buccal aspect, with 2.36 +/- 0.94 mm at baseline, 3.35 +/- 1.00 mm after 4 months and 3.23 +/- 0.77 mm after 1 year (P < 0.05), whereas no change was observed in the implant group. The difference between the two groups was significant after 4 months and 1 year (P < 0.05). The implant + connective tissue graft group also showed a greater reduction in buccal defects after 1 year compared to the implant group (Delta -0.50 +/- 0.70 mm and Delta -1.80 +/- 1.30 mm, respectively; P < 0.05). After 1 year, a significant intergroup difference in proximal bone resorption was observed, with the implant + connective tissue graft group showing less bone resorption compared to the implant group (0.75 +/- 0.20 mm and 0.92 +/- 0.30 mm, respectively; P < 0.05). Conclusions: Placement of a connective tissue graft simultaneous to single implant insertion in the anterior maxillary region can increase the thickness of the peri-implant mucosa and reduce proximal bone resorption.
  • ItemArtigo
    Miniscrew insertion sites of infrazygomatic crest and mandibular buccal shelf in different vertical craniofacial patterns: A cone-beam computed tomography study
    (2021-11-01) Matias, Murilo ; Flores-Mir, Carlos ; de Almeida, Márcio Rodrigues ; Vieira, Bruno da Silva ; de Freitas, Karina Maria Salvatore ; Nunes, Daniela Calabrese ; Ferreira, Marcos Cezar ; Ursi, Weber ; Guarulhos University ; University of Alberta ; Universidade Norte do Paraná ; Private Practice ; Ingá Dental School ; Universidade Estadual Paulista (UNESP)
    Objective: To identify optimal areas for the insertion of extra-alveolar miniscrews into the infrazygomatic crest (IZC) and mandibular buccal shelf (MBS), using cone beam computed tomography (CBCT) imaging in patients with different craniofacial patterns. Methods: CBCT reconstructions of untreated individuals were used to evaluate the IZC and MBS areas. The participants were divided into three groups, based on the craniofacial pattern, namely, brachyfacial (n = 15; mean age, 23.3 years), mesofacial (n = 15; mean age, 19.24 years), and dolichofacial (n = 15; mean age, 17.79 years). In the IZC, the evaluated areas were at 11, 13, and 15 mm above the buccal cusp tips of the right and left first molars. In the MBS, the evaluated areas were at the projections of the first molars’ distal roots and second molars’ mesial and distal roots, at a 4-and 8-mm distance from the cementoenamel junction. Intergroup comparisons were performed with analysis of variance and the Tukey test. Results: There was no statistically significant difference in the IZC bone thickness among the groups. For MBS bone availability, some comparisons revealed no difference; meanwhile, other comparisons revealed increased MBS bone thickness in the brachyfacial (first molars distal roots) and dolichofacial (second molars mesial and distal roots) patterns. Conclusions: There was no significant difference in the IZC bone thickness among the groups. The facial skeletal pattern may affect the availability of ideal bone thickness for the insertion of extra-alveolar miniscrews in the MBS region; however, this variability is unlikely to be clinically meaningful. [Korean J Orthod 2021;51(6):387-396].
  • ItemArtigo
    Pulpal temperature increase with high-speed handpiece, Er:YAG laser and ultrasound tips
    (2008-01-01) Mollica, Fernanda Brandão ; Camargo, Fernanda Pelogia ; Zamboni, Sandra Costa ; Pereira, Sarina Maciel Braga ; Teixeira, Symone Cristina ; Nogueira Jr., Lafayette ; Universidade Estadual Paulista (UNESP)
    The aim of this study was to compare intrapulpal temperature increase produced by high-speed handpiece, Er:YAG laser and CVDentus ultrasound tips during cavity preparation. Thirty bovine mandibular incisors with an enamel/dentin thickness of 4 mm at buccal surface had their roots amputated and were allocated to the following groups (n=10): Group I- high-speed handpiece; Group II- noncontact Er:YAG laser (250 mJ/4Hz); and Group III- CVDentus ultrasouns tips. All devices were used with water cooling. Class V cavities were prepared to a depth of 3.5 mm, measured with a periodontal probe. A type T thermocouple was placed inside the pulp chamber to determine the temperature increase (°C), which was recorded by a data acquisition system ADS 2000 IP (Lynx Technology) linked to a notebook computer. Data were analyzed statistically by oneway ANOVA and Tukey's test (p=0.05). The mean temperature rises were: 1.10°C (±0.56) for Group I, 0.84°C (±0.55) for Group II, and 3.00°C (± 1.34) for Group III. There were no statistically significant differences (p>0.05) between Groups I and II, but both of them differed significantly from Group III (p<0.05). In conclusion, the use of Er:YAG laser and high-speed handpiece for cavity preparation resulted in similar temperature increase. Although ultrasound tips generated significantly higher intrapulpal temperature increase, it remained below the critical value of 5.5°C and may be considered safe for use.
  • ItemArtigo
    Dentin bond strength: Influence of Er: YAG and Nd: YAG lasers
    (2013-01-01) Ribeiro, Carolina Ferraz ; De Paiva Gonçalves, Sérgio Eduardo ; Yui, Karen Cristina Kazue ; Borges, Alessandra Bühler ; Barcellos, Daphne Câmara ; Brayner, Ricardo ; Universidade Estadual Paulista (UNESP)
    The aim of this study was to investigate the effects of Er:YAG and Nd:YAG lasers on the shear bond strength of composite resin to dentin. The coronal portion of 56 human molars was divided into three parts, and the dentin thickness was standardized at 2 mm. A 3-mm hole was marked in the center of each tooth with sealing tape paper. The specimens (n = 14) were then divided into four groups: (1) acid etching + Single Bond (SB) (control), (2) acid etching + SB + Nd:YAG laser irradiation (before adhesive curing), (3) thermal etching with the Er:YAG laser + SB, and (4) thermal etching with the Er:YAG laser + SB + Nd:YAG laser irradiation (before adhesive curing). A composite resin cylinder was built into the delimited area for conducting the shear bond strength test on the universal testing machine. The means ± standard deviations were: group 1, 17.05 ± 4.15 MPa; group 2, 16.90 ± 3.36 MPa; group 3, 12.12 ± 3.85 MPa; and group 4, 12.92 ± 2.73 MPa. Groups 1 and 2 presented significantly higher values than groups 3 and 4. It was concluded that conventional etching with 37% phosphoric acid yielded significantly higher bond strength values compared to thermal etching with the Er:YAG laser. The Nd:YAG laser did not significantly influence the bond strength. © 2013 by Quintessence Publishing Co Inc.
  • ItemArtigo
    A comparative study of microstrain around three-morse taper implants with machined and plastic copings under axial loading
    (2013-01-01) de Abreu, Celina Wanderley ; de Vasconcellos, Luis Gustavo Oliveira ; Balducci, Ivan ; Nishioka, Renato Sussumu ; Universidade Estadual de Campinas (UNICAMP) ; Universidade Estadual Paulista (UNESP)
    Aim: To evaluate the occurrence of microstrain around morse taper implants in straight configuration under axial load in a cast monoblock framework. Methods: Three implants were inserted in a polyurethane block and microunit abutments were installed on the implants with 20 Ncm torque. Plastic and machined copings were adapted on the preset waxing to fabricate the framework (n=5). Four strain gauges were attached on the upper surface of the block and then each framework was tightened on the abutments and a vertical load of 30 kg was applied to five points of the framework. Results: The data obtained in the strain gauge analysis were subjected to twoway ANOVA and Tukey's test (α=0.05). There was statistically significant difference (p=0.0222) for the factor application point and the mean microstrain values were: application point B 402,04μσ, point A 401.21μσ, point E 390.44μσ , point D 341.76μσ and point C 309.19 μσ. Conclusions: There was no microstrain difference between plastic and machined copings during axial loading. Difference in the application point was observed, but remained within bone physiological limits.
  • ItemArtigo
    Finite element analysis and fracture resistance testing of a new intraradicular post
    (2012-01-01) Yamamoto, Eron Toshio Colauto ; Pagani, Clovis ; da Silva, Eduardo Galera ; Noritomi, Pedro Yoshito ; Uehara, André Yugou ; Kemmoku, Daniel Takanori ; Universidade Estadual Paulista (UNESP) ; Universidade Estadual de Campinas (UNICAMP)
    ObjectiveS: The objective of the present study was to evaluate a prefabricated intraradicular threaded pure titanium post, designed and developed at the São José dos Campos School of Dentistry - UNESP, Brazil. This new post was designed to minimize stresses observed with prefabricated post systems and to improve cost-benefits. Material and Methods: Fracture resistance testing of the post/core/root complex, fracture analysis by microscopy and stress analysis by the finite element method were used for post evaluation. The following four prefabricated metal post systems were analyzed: group 1, experimental post; group 2, modification of the experimental post; group 3, Flexi Post, and group 4, Para Post. For the analysis of fracture resistance, 40 bovine teeth were randomly assigned to the four groups (n=10) and used for the fabrication of test specimens simulating the situation in the mouth. The test specimens were subjected to compressive strength testing until fracture in an EMIC universal testing machine. After fracture of the test specimens, their roots were sectioned and analyzed by microscopy. For the finite element method, specimens of the fracture resistance test were simulated by computer modeling to determine the stress distribution pattern in the post systems studied. Results: The fracture test presented the following averages and standard deviation: G1 (45.63±8.77), G2 (49.98±7.08), G3 (43.84±5.52), G4 (47.61±7.23). Stress was homogenously distributed along the body of the intraradicular post in group 1, whereas high stress concentrations in certain regions were observed in the other groups. These stress concentrations in the body of the post induced the same stress concentration in root dentin. ConclusionS: The experimental post (original and modified versions) presented similar fracture resistance and better results in the stress analysis when compared with the commercial post systems tested (08/2008-PA/CEP).
  • ItemArtigo
    In vitro evaluation of the microhardness of bovine enamel exposed to acid solutions after bleaching
    (2011-11-01) Zanet, Caio Gorgulho ; Fava, Marcelo ; Alves, Levy Anderson César ; Ibirapuera University - UNIB ; Universidade Estadual Paulista (UNESP)
    Acid erosion is a superficial loss of enamel caused by chemical processes that do not involve bacteria. Intrinsic and extrinsic factors, such as the presence of acid substances in the oral cavity, may cause a pH reduction, thus potentially increasing acid erosion. The aim of this study was to evaluate the microhardness of bleached and unbleached bovine enamel after immersion in a soda beverage, artificial powder juice and hydrochloric acid. The results obtained for the variables of exposure time, acid solution and substrate condition (bleached or unbleached enamel) were statistically analyzed by the ANOVA and Tukey tests. It was concluded that a decrease in microhardness renders dental structures more susceptible to erosion and mineral loss, and that teeth left unbleached show higher values of microhardness compared to bleached teeth.
  • ItemArtigo
    Comparative study of oral mucosa micronuclei in smokers and alcoholic smokers
    (2012-02-01) Oliveira, Lidiana Ubiña ; Lima, Celina Faig ; Salgado, Miguel Ângelo Castillo ; Balducci, Ivan ; Almeida, Janete Dias ; Universidade Estadual Paulista (UNESP)
    Objective: To compare the frequency of micronuclei and metanucleated anomalies in the oral mucosa of smokers, alcoholic smokers, and nonsmokers. Study Design: Three groups were studied: group I, 15 smokers; group II, 16 alcoholic smokers; and group III, 20 nonsmokers. Three smears from the lateral left border of the tongue were processed for Feulgen staining. A minimum of 300 cells per participant were examined for the quantification of micronuclei and metanucleated anomalies. Results: The Kruskal-Wallis test showed no significant difference in the frequency of micronuclei (p = 0.602) or karyorrhexis (p = 0.114) among the three groups, but there was a significant difference in the frequency of broken eggs, binucleated cells, and karyolysis (p=0.001). Spearman's correlation indicated an influence of the number of cigarettes per day on micronuclei frequency. Tobacco caused significant alterations in the exfoliative cytology (broken eggs, binucleated cells, and karyolysis) of chronic smokers, but not in the frequency of micronuclei or karyorrhexis, despite the observation of a larger absolute number of micronuclei in group II. Conclusion: The action of genotoxic agents (tobacco and alcohol) causes alterations in the frequency of micronuclei and metanucleated anomalies. © Science Printers and Publishers, Inc.
  • ItemArtigo
    Effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones
    (2011-01-01) Salvia, Ana Carolina Rodrigues Danzi ; Teodoro, Guilherme Rodrigues ; Balducci, Ivan ; Koga-Ito, Cristiane Yumi ; de Oliveira, Simone Helena Gonçalves ; Universidade Estadual Paulista (UNESP)
    The aim of this study was to evaluate the effectiveness of 2% peracetic acid for the disinfection of gutta-percha cones contaminated in vitro with Escherichia coli, Staphylococcus aureus, Streptococcus mutans, Candida albicans and Bacillus subtilus (in spore form). Two hundred and twenty-five gutta-percha cones were contaminated with standardized suspensions of each microorganism and incubated at 37°C for 24 h. The cones were divided into 10 experimental groups (n = 15), according to the microorganism tested and disinfection testing times. The disinfection procedure consisted of immersing each cone in a plastic tube containing the substance. The specimens remained in contact with the substance for 1 or 2.5 minutes. Afterwards, each cone was transferred to a 10% sodium thiosulphate solution (Na2S2O3) to neutralize the disinfectant. Microbial biofilms adhering to the cones were dispersed by agitation. Aliquots of 0.1 ml of the suspensions obtained were plated on Sabouraud dextrose agar, or brain and heart infusion agar, and incubated at 37°C for 24 h. The results were expressed in colony forming units (CFU/ml) and the data were submitted to the Wilcoxon Signed Rank Test (level of significance at 0.05). A significant reduction was observed, after 1 minute of exposure, in the test solution for C. albicans (p = 0.0190), S. aureus (p = 0.0001), S. mutans (p = 0.0001), B. subtilis (p = 0.0001), and E. coli (p = 0.0001). After 2.5 minutes of exposure, 100% of the microbial inocula were eliminated. It was concluded that the 2% peracetic acid solution was effective against the biofilms of the tested microorganisms on gutta-percha cones at 1 minute of exposure.
  • ItemArtigo
    Cytokeratin profile in exfoliative cytology of smokers
    (2011-02-01) Lima, Celina Faig ; Leite, Sabrina Ferreira Pinotti ; Carvalho, Yasmin Rodarte ; Cabral, Luiz Antonio Guimarães ; Balducci, Ivan ; Almeida, Janete Dias ; Universidade Estadual Paulista (UNESP)
    OBJECTIVE: To compare the expression of cytokeratins (CKs) 6, 16, 19 and pan-cytokeratin (PAN) in oral mucosa cells between smokers and nonsmokers to determine the proliferative activity and expression indicative of a potential for malignant transformation. STUDY DESIGN: Smears were obtained from the left lateral border of the tongue with a cytobrush from 25 smokers and 20 nonsmokers seen at the clinics of São José dos Campos Dental School, São Paulo State University, São José dos Campos, São Paulo, Brazil, and processed for immunohistochemistry. Conventional microscopy was used for qualitative analysis. Proportions were compared statistically by the z-test and Fisher's exact test. RESULTS: The expression of CK6 (p = 0.002), CK16 (p=0.003), CK19 (p=0.0001) and PAN (p = 0.008) was higher in oral mucosa smears from smokers compared to nonsmokers. CONCLUSION: The expression of CK6 and CK16 demonstrated increased epithelial proliferation in the oral mucosa of smokers, and expression of CK19 indicated alterations in epithelial maturation. The expression of PAN indicates the need for the investigation of other types of CK in further studies. © Science Printers and Publishers, Inc.
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    The adhesive system and root canal region do not influence the degree of conversion of dual resin cement
    (2010-01-01) Pereira, Priscilla Cristoforides ; de Melo, Renata Marques ; Chaves, Carolina ; Galhano, Graziela A. P. ; Bottino, Marco Antonio ; Balducci, Ivan ; Universidade Estadual Paulista (UNESP)
    Objectives: The aim of this study was to evaluate the influence of two adhesive systems and the post space region on the degree of conversion of dual resin cement and its bond strength to root dentin. Material and Methods: One three-step etch-and-rinse (All-bond 2, Bisco) and another one-step self-etch (Xeno III, Dentsply) adhesive systems were applied on 20 (n=10) crownless bovine incisors, at 12-mm-deep post space preparation, and a fiber post (DT Light Post, Bisco) was cemented using a dual cure resin cement (Duo-Link, Bisco). Three transverse sections (3 mm) were obtained, being one from each study region (cervical, middle and apical). The degree of conversion of the dual cure resin cement was determined by a micro-Raman spectrometer. The data (%) were submitted to repeated-measures analysis of variance and Tukey's test (p<0.05). Results: For both groups, the degree of conversion means (%) (All bond 2cervical = 69.3; All bond 2middle = 55.1; All bond 2apical= 56; Xeno III cervical = 68.7; Xeno IIImiddle = 68.8; Xeno III apical = 54.3) were not significantly different along the post space regions (p<0.05). Conclusions: Neither the adhesive nor the post space region influenced the degree of conversion of the cement layer.
  • ItemArtigo
    Machined and plastic copings in three-element prostheses with different types of implant-abutment joints: A strain gauge comparative analysis
    (2010-01-01) Nishioka, Renato Sussumu ; Nishioka, Lea Nogueira Braulinode de Melo ; Abreu, Celina Wanderley ; de Vasconcellos, Luis Gustavo Oliveirade ; Balducci, Ivan ; Universidade Estadual Paulista (UNESP)
    Objective: Using strain gauge (SG) analysis, the aim of this in vitro study was quantify the strain development during the fixation of three-unit screw implant-supported fixed partial dentures, varying the types of implant-abutment joints and the type of prosthetic coping. The hypotheses were that the type of hexagonal connection would generate different microstrains and the type of copings would produce similar microstrains after prosthetic screws had been tightened onto microunit abutments. Materials and methods: Three dental implants with external (EH) and internal (IH) hexagonal configurations were inserted into two polyurethane blocks. Microunit abutments were screwed onto their respective implant groups, applying a torque of 20 Ncm. Machined Co-Cr copings (M) and plastic prosthetic copings (P) were screwed onto the abutments, which received standard wax patterns. The wax patterns were cast in Co-Cr alloy (n = 5), forming four groups: Gl) EH/M; G2) EH/P; G3) IH/M and G4) IH/P. Four SGs were bonded onto the surface of the block tangentially to the implants, SG 1 mesially to implant 1, SG 2 and SG 3 mesially and distally to implant 2, respectively, and SG 4 distally to implant 3. The superstructure's occlusal screws were tightened onto microunit abutments with 10 Ncm torque using a manual torque driver. The magnitude of microstrain on each SG was recorded in units of microstrain (me)- The data were analyzed statistically by ANOVA and Tukey's test (p<0.05). Results: Microstrain values of each group were: Gl= 338.1±223.0 μe{open}; G2= 363.9±190.9 μe{open}; G3= 415.1±53.5 μe{open}; G4= 363.9± 190.9 μe{open}. No statistically significant difference was found between EH and IH, regardless of the type of copings (p>0.05). The hypotheses were partially accepted. Conclusions: It was concluded that the type of hexagonal connection and coping presented similar mechanical behavior under tightening conditions.
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    Bond strength durability of self-etching adhesives and resin cements to dentin
    (2009-01-01) Lima Chaves, Carolina De Andrade ; De Melo, Renata Marques ; Passos, Sheila Pestana ; Camargo, Fernanda Pelógia ; Bottino, Marco Antonio ; Balducci, Ivan ; Universidade Estadual Paulista (UNESP) ; Dental School of São José Dos Campos ; Dental Materials and Prosthodontics Department
    objectives: To evaluate the microtensile bond strength (μTBS) of one- (Xeno III, Dentsply) and two-step (Tyrian-One Step Plus, Bisco) self-etching adhesive systems bonded to dentin and cemented to chemically cured (C&B Metabond) or light-cured paste of a dual-cure resin cement (Variolink II, Ivoclar) within a short (24 h) and long period of evaluation (90 days). Material and Methods: Forty recently extracted human molars had their roots removed and their occlusal dentin exposed and ground wet with 600-grit SiC paper. After application of one of the adhesives, the resin cement was applied to the bonded surface and a composite resin block was incrementally built up to a height of 5 mm (n=10). The restored teeth were stored in distilled water at 37°C for 7 days. The teeth were then cut along two axes (x and y), producing beam-shaped specimens with 0.8 mm2 cross-sectional area, which were subjected to μTBS testing at a crosshead speed of 0.05 mm/min and stressed to failure after 24 h or 90 days of storage in water. The μTBS data in MPa were subjected to three-way analysis of variance and Tukey's test (a= 0.05). Results: The interaction effect for all three factors was statistically significant (three-way ANOVA, p<0.001). All eight experimental means (MPa) were compared by the Tukey's test (p<0.05) and the following results were obtained: Tyrian-One Step Plus /C&B/24 h (22.4±7.3); Tyrian-One Step Plus /Variolink II/24 h (39.4±11.6); Xeno III/C&B/24 h (40.3±12.9); Xeno III/Variolink II/24 h (25.8±10.5); Tyrian-One Step Plus / C&B/90 d (22.1±12.8) Tyrian-One Step Plus/VariolinkII/90 d (24.2±14.2); Xeno III/C&B/90 d (27.0±13.5); Xeno III/Variolink II/ 90 d (33.0±8.9). Conclusions: Xeno III/Variolink II was the luting agent/adhesive combination that provided the most promising bond strength after 90 days of storage in water.
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    Roughness of human enamel surface submitted to different prophylaxis methods
    (2008-01-01) Castanho, Gisela Muassab ; Arana-Chavez, Victor E. ; Fava, Marcelo ; Universidade de São Paulo (USP) ; Universidade Estadual Paulista (UNESP)
    The purpose of this in vitro study was to evaluate alterations in the surface roughness and micromorphology of human enamel submitted to three prophylaxis methods. Sixty-nine caries-free molars with exposed labial surfaces were divided into three groups. Group I was treated with a rotary instrument set at a low speed, rubber cup and a mixture of water and pumice; group II with a rotary instrument set at a low speed, rubber cup and prophylaxis paste Herjos-F (Vigodent S/A Indústria e Comércio, Rio de Janeiro, Brazil); and group III with sodium bicarbonate spray Profi II Ceramic (Dabi Atlante Indústrias Médico Odontológicas Ltda, Ribeirão Preto, Brazil). All procedures were performed by the same operator for 10 s, and samples were rinsed and stored in distilled water. Pre and post-treatment surface evaluation was completed using a surface profilometer (Perthometer S8P, Mark, Perthen, Germany) in 54 samples. In addition, the other samples were coated with gold and examined in a scanning electron microscope (SEM). The results of this study were statistically analyzed with the paired t-test (Student), the Kruskal-Wallis test and the Dunn (5%) test. The sodium bicarbonate spray led to significantly rougher surfaces than the pumice paste. The use of prophylaxis paste showed no statistically significant difference when compared with the other methods. Based on SEM analysis, the sodium bicarbonate spray presented an irregular surface with granular material and erosions. Based on this study, it can be concluded that there was an increased enamel surface roughness when teeth were treated with sodium bicarbonate spray when compared with teeth treated with pumice paste.
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    Peripheral giant cell granuloma in anterior maxilla: Case report in a child
    (2005-01-01) Martins, Manoela Domingues ; Pires, Flávio ; Daleck, Fernanda ; Myaki, Silvio Issao ; Friggi, Maria Naira Pereira ; Martins, Marco Antonio Trevizani ; Nove de Julho University - UNINOVE ; Universidade Estadual Paulista (UNESP) ; Braz Cubas University
    A case of giant cell granuloma in a 7-year-old boy was reported. The lesion was probably caused by trauma and was interfering with the normal eruption of the permanent maxillary left lateral incisor. Differential diagnosis and treatment of this lesion are discussed.
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    Histopathology of the pulp of primary molars with active and arrested dentinal caries
    (2000-12-01) Nicolo, Rebeca Di ; Universidade Estadual Paulista (UNESP)
    The purpose of this study was to compare the histological appearance of the pulp of human primary molars with active and arrested lesions. The sample consisted of 36 primary molars (18 with active lesions and 18 with arrested lesions) extracted from 35 children between 5 to 9 years of age. The histological diagnosis was classified in normal pulp, transitional stage, partial pulpitis, total pulpitis and total necrosis, and then subdivided in three subgroups: treatable, untreatable and questionable. Results showed that normal pulp or transitional stage (treatable category) was diagnosed in 50% of teeth with arrested lesions, compared to 11.1% of teeth with active lesions. Partial pulpitis (questionable category) was present in 38.8% with arrested lesions compared to 22.2% with active lesions. Total pulpitis and total necrosis (untreatable category) was diagnosed in 11.2% with arrested lesions compared to 66.7% with active lesions. The observed frequencies of histological categories between both groups were statistically significant (P < 0.05). Histologically, pulp reaction under active and arrested lesions in primary molars revealed the formation of a basophilic calcio-traumatic line at the junction of the primary and reparative dentin, formation of reparative dentin and a regular odontoblastic layer in 60% of the cases. Results indicated that the type of lesion (active or arrested) is a good indicator of the histological status of the pulp.
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    An interview with David Normando
    (Dental Press International, 2019-05-20) Normando, David ; Flores-mir, Carlos ; Faber, Jorge ; Capelozza, Leopoldino ; Ursi, Weber ; Federal University of Pará ; University of Alberta ; Brasília University ; Sagrado Coração University ; Universidade Estadual Paulista (Unesp)
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    Possíveis fatores etiológicos para desordens temporomandibulares de origem articular com implicações para diagnóstico e tratamento
    (Dental Press International, 2010-06) Maydana, Aline Vettore ; Tesch, Ricardo De Souza ; Denardin, Odilon Vitor Porto ; Ursi, Weber José Da Silva ; Dworkin, Samuel Franklin ; Faculdade de Medicina de Petrópolis ; Washington University ; Universidade Estadual Paulista (Unesp) ; Hospital Heliópolis
    Os fatores envolvidos na etiologia, diagnóstico e tratamento das desordens temporomandibulares (DTM) de origem articular foram revisados. Critérios específicos de inclusão e exclusão para o diagnóstico de DTM são essenciais, mas apresentam utilidade limitada. Atualmente, os Critérios Diagnósticos de Pesquisa para Desordem Temporomandibular (RDC/TMD) oferecem a melhor classificação baseada em evidências para os subgrupos mais comuns de DTM. O RDC/TMD inclui não apenas métodos para a classificação diagnóstica física, presentes em seu Eixo I, mas ao mesmo tempo métodos para avaliar a intensidade e a severidade da dor crônica e os níveis de sintomas depressivos e físicos não-específicos, presentes em seu Eixo II. Embora historicamente as más oclusões tenham sido consideradas como fatores de risco para o desenvolvimento das DTM, incluindo as predominantemente articulares, em muitos casos a associação estabelecida entre essas variáveis parece ter tomado direção oposta. No que diz respeito aos desarranjos internos da ATM, os resultados de estudos prévios sobre a redução induzida do ramo mandibular, secundária ao deslocamento anterior do disco articular, indicam que o reposicionamento do disco deslocado em crianças ou adolescentes jovens pode fazer mais sentido do que previamente imaginado. O uso terapêutico de suplementos alimentares, como o sulfato de glicosamina, parece uma alternativa segura ao uso dos medicamentos anti-inflamatórios normalmente utilizados para controlar a dor relacionada à osteoartrite da articulação temporomandibular (ATM), embora a evidência em torno de sua eficácia para a maioria dos pacientes de DTM não tenha sido completamente estabelecida.
  • ItemArtigo
    Influence of topical acidulated phosphate fluoride on surface roughness of human enamel and different restorative materials
    (Pontifícia Universidade Católica do Rio Grande do Sul, 2010) Botta, Ana Carolina ; Mollica, Fernanda Brandão ; Ribeiro, Carolina Ferraz ; Araujo, Maria Amélia Máximo De ; Nicoló, Rebeca Di ; Balducci, Ivan ; Universidade Estadual Paulista (Unesp)
    PURPOSE: The aim of this study was to assess the influence of topical acidulated phosphate fluoride on the surface roughness of both human enamel and different restorative materials. METHODS: Resin-modified glass ionomer (Vitremer - V), microhybrid composite resin with fluoride (Tetric Ceram - T), and nanofiller composite resin without fluoride (Z350 - Z) were evaluated. Acidulated phosphate fluoride (APF, 1.23%) was applied to half of the specimens (groups VF, TF, ZF and EF). Ten intact human third molars were used to obtain enamel specimens (E). Six experimental groups (n=10) and two control groups (n=10) were formed. The mean surface roughness (Ra) of both the restorative materials and enamel, with and without fluoride, was evaluated with a profilometer. Data were analyzed with an ANOVA, Tukey's test, Student's t-test, and Dunn-Bonferroni test at the 5% significance level. RESULTS: The mean values of surface roughness, in micrometers, were as follows: Z=0.60; TF=1.00; ZF=1.05; VF=1.18; T=2.10; V=2.70; E=16.99; and EF=21.19. Both E and EF presented significantly higher surface roughness than the other experimental groups. CONCLUSION: It was concluded that APF increased the surface roughness of enamel and decreased the surface roughness of both resin-modified glass-ionomer cement and microhybrid composite resin with fluoride. The surface roughness of the nanofiller composite resin was not modified by APF.