ItemArtigoRecovery of kicking kinematics and performance following repeated high-intensity running bouts in the heat: Can a rapid local cooling intervention help young soccer players?(2023-01-01) Palucci Vieira, Luiz H. [UNESP]; Carling, Christopher; Kalva-Filho, Carlos A. [UNESP]; Santinelli, Felipe B. [UNESP]; Velluto, Lorenzo A. G. [UNESP]; da Silva, João Pedro [UNESP]; Clemente, Filipe M; Kellis, Eleftherios; Barbieri, Fabio A [UNESP]; Universidade Estadual Paulista (UNESP); Clairefontaine National Football Centre; French Institute of Sport (INSEP); Hasselt University; Instituto Politécnico de Viana Do Castelo; Aristotle University of ThessalonikiThe effects of a cooling strategy following repeated high-intensity running (RHIR) on soccer kicking performance in a hot environment (>30ºC) were investigated in youth soccer players. Fifteen academy under-17 players participated. In Experiment 1, players completed an all-out RHIR protocol (10×30 m, with 30s intervals). In Experiment 2 (cross-over design), participants performed this running protocol under two conditions: (1) following RHIR 5 minutes of cooling where ice packs were applied to the quadriceps/hamstrings, (2) a control condition involving passive resting. Perceptual measures [ratings of perceived exertion (RPE), pain and recovery], thigh temperature and kick-derived video three-dimensional kinematics (lower limb) and performance (ball speed and two-dimensional placement indices) were collected at baseline, post-exercise and intervention. In Experiment 1, RHIR led to small-to-large impairments (p < 0.03;d = −0.42–-1.83) across perceptual, kinematic and performance measures. In experiment 2, RPE (p < 0.01; Kendall’s W = 0.30) and mean radial error (p = 0.057; η2 = 0.234) increased only post-control. Significant small declines in ball speed were also observed post-control (p < 0.05; d = 0.35). Post-intervention foot centre-of-mass velocity was moderately faster in the cooling compared to control condition (p = 0.04; d = 0.60). In youth soccer players, a short cooling period was beneficial in counteracting declines in kicking performance, in particular ball placement, following intense running activity in the heat. ItemCartaAquatic exercise in adults with chronic disease: Evidence of benefit for individuals with hypertension(2023-01-01) Ciolac, Emmanuel Gomes [UNESP]; Universidade Estadual Paulista (UNESP) ItemArtigoGait Training with Functional Electrical Stimulation Improves Mobility in People Post-Stroke(2023-05-01) Dantas, Maria Tereza Artero Prado; Fernani, Deborah Cristina Gonçalves Luiz; Silva, Talita Dias da; Assis, Iramaia Salomão Alexandre de [UNESP]; Carvalho, Augusto Cesinando de [UNESP]; Silva, Sidney Benedito; Abreu, Luiz Carlos de; Barbieri, Fabio Augusto [UNESP]; Monteiro, Carlos Bandeira de Mello; ABC Faculty of Medicine; Universidade de São Paulo (USP); University of West Paulista (UNOESTE); University City of Sao Paulo (UNICID); Universidade Estadual Paulista (UNESP); Federal University of Espírito Santo (UFES)(1) Background: Stroke is one of the leading causes of disability. To identify the best treatment strategies for people with stroke (PwS), the aim of the current study was to compare the effects of training on a treadmill with functional electrical stimulation (TT-FES) with training on a treadmill (TT), and to analyze the effects of sequence of training on mobility and the parameters of walking ability. (2) Methods: Prospective, longitudinal, randomized and crossover study, in which 28 PwS were distributed into groups, namely the A-B Group (TT-FES followed by TT) and B-A Group (TT followed by TT-FES), using the foot drop stimulator, and were measured with functional tests. (3) Results: We found improved mobility, balance, non-paretic limb coordination, and endurance only in the group that started with TT-FES. However, sensorimotor function improved regardless of the order of training, and paretic limb coordination only improved in the B-A Group, but after TT-FES. These data indicate that the order of the protocols changed the results. (4) Conclusions: Although biomechanical evaluation methods were not used, which can be considered a limitation, our results showed that TT-FES was superior to isolated training on a treadmill with regard to balance, endurance capacity, and coordination of the non-paretic limb. ItemArtigo(A)symmetry during gait initiation in people with Parkinson’s disease: A motor and cortical activity exploratory study(2023-01-01) Faria, Murilo Henrique [UNESP]; Simieli, Lucas [UNESP]; Rietdyk, Shirley; Penedo, Tiago [UNESP]; Santinelli, Felipe Balistieri [UNESP]; Barbieri, Fabio Augusto [UNESP]; Universidade Estadual Paulista (UNESP); Purdue University; Hasselt UniversityBackground: Gait asymmetry and deficits in gait initiation (GI) are among the most disabling symptoms in people with Parkinson’s disease (PwPD). Understanding if PwPD with reduced asymmetry during GI have higher asymmetry in cortical activity may provide support for an adaptive mechanism to improve GI, particularly in the presence of an obstacle. Objective: This study quantified the asymmetry of anticipatory postural adjustments (APAs), stepping parameters and cortical activity during GI, and tested if the presence of an obstacle regulates asymmetry in PwPD. Methods: Sixteen PwPD and 16 control group (CG) performed 20-trials in two conditions: unobstructed and obstructed GI with right and left limbs. We measured, through symmetry index, (i) motor parameters: APAs and stepping, and (ii) cortical activity: the PSD of the frontal, sensorimotor and occipital areas during APA, STEP-I (moment of heel-off of the leading foot in the GI until the heel contact of the same foot); and STEP-II (moment of the heel-off of the trailing foot in the GI until the heel contact of the same foot) phases. Results: Parkinson’s disease showed higher asymmetry in cortical activity during APA, STEP-I and STEP-II phases and step velocity (STEP-II phase) during unobstructed GI than CG. However, unexpectedly, PwPD reduced the level of asymmetry of anterior–posterior displacement (p < 0.01) and medial-lateral velocity (p < 0.05) of the APAs. Also, when an obstacle was in place, PwPD showed higher APAs asymmetry (medial-lateral velocity: p < 0.002), with reduced and increased asymmetry of the cortical activity during APA and STEP-I phases, respectively. Conclusion: Parkinson’s disease were not motor asymmetric during GI, indicating that higher cortical activity asymmetry can be interpreted as an adaptive behavior to reduce motor asymmetry. In addition, the presence of obstacle did not regulate motor asymmetry during GI in PwPD. ItemArtigoTranslation, Cross-Cultural Adaptation, and Validation of the Physical Activity Scale for Individuals With Physical Disabilities for Brazilian Individuals With Parkinson's Disease(2023-04-01) Golin, Andrea; Costa, Elisa de Carvalho [UNESP]; Assis, Iramaia Salomão Alexandre de [UNESP]; Makhoul, Marina Portugal; Barbieri, Fabio Augusto [UNESP]; Torriani-Pasin, Camila; Universidade de São Paulo (USP); Universidade Estadual Paulista (UNESP)The Physical Activity Scale for Individuals with Physical Disabilities (PASIPD) is not available to Portuguese-Brazil. This study translates, cross-culturally adapts, and validates the PASIPD for Brazilian individuals with Parkinson's disease. The translation process followed guidelines: initial translation, synthesis, back translation, expert committee, and pretest. The validation and reliability processes were conducted with 40 individuals (15 men and 25 women) with Parkinson's disease. Concurrent validity was evaluated between PASIPD to Brazilian Portuguese, International Physical Activity Questionnaire, and Human Activity Profile. PASIPD to Brazilian Portuguese was found to be moderately correlated with International Physical Activity Questionnaire (r = .474, p < .05); however, there was no correlation with Human Activity Profile (r = .271, p < .05). We used the intrarater reliability with intraclass correlation coefficient and test-retest. Intrarater reliability was high (intraclass correlation coefficient = .80). Internal consistency was considered adequate by Cronbach's alpha (α = .70). PASIPD to Brazilian Portuguese is a valid and reliable instrument for evaluating physical activity levels in Brazilian individuals with Parkinson's disease. ItemArtigoMechanical energy on anaerobic capacity during a supramaximal treadmill running in men: Is there influence between runners and active individuals?(2023-03-01) Zagatto, Alessandro Moura [UNESP]; González, Joel Abraham Martínez [UNESP]; de Poli, Rodrigo Araujo Bonetti [UNESP]; Barbieri, Fabio Augusto [UNESP]; Bloedow, Leonardo de los Santos; Peyré-Tartaruga, Leonardo; Universidade Estadual Paulista (UNESP); Universidad Autónoma del Estado de México; Exercise Research LaboratoryThis study verified whether mechanical variables influence the anaerobic capacity outcome on treadmill running and whether these likely influences were dependent of running experience. Seventeen physical active and 18 amateur runners, males, performed a graded exercise test and constant load exhaustive running efforts at 115% of intensity associated to maximal oxygen consumption. During the constant load were determined the metabolic responses (i.e., gas exchange and blood lactate) to estimate the energetic contribution and anaerobic capacity as well as kinematic responses. The runners showed higher anaerobic capacity (16.6%; p = 0.005), but lesser time to exercise failure (−18.8%; p = 0.03) than active subjects. In addition, the stride length (21.4%; p = 0.00001), contact phase duration (−11.3%; p = 0.005), and vertical work (−29.9%; p = 0.015). For actives, the anaerobic capacity did not correlate significantly with any physiologic, kinematic, and mechanical variables and no regression model was fitted using the stepwise multiple regression, while to runners the anaerobic capacity was significantly correlated with phosphagen energetic contribution (r = 0.47; p = 0.047), external power (r = −0.51; p = 0.031), total work (r = −0.54; p = 0.020), external work (r = −0.62; p = 0.006), vertical work (r = −0.63; p = 0.008), and horizontal work (r = −0.61; p = 0.008), and the vertical work and phosphagen energetic contribution presented a coefficient of determination of 62% (p = 0.001). Based on findings, it is possible to assume that for active subjects, the mechanical variables have no influence over the anaerobic capacity, however, for experienced runners, the vertical work and phosphagen energetic contribution have relevant effect over anaerobic capacity output. ItemArtigoComparison between mood states, stress and recovery in CrossFit® competitors and non-competitors(2022-11-01) D’alpino, Isabela Ambrósio; Moterosso, João Paulo Campanhã; Botaro, Weligton Rodrigo; DA SILVA, Alini Oliveira Cassiano; Sant’anna, Paula Grippa; Testa Junior, Ademir; Verardi, Carlos Eduardo Lopes [UNESP]; Zanini, GABRIEL DE SOUZA [UNESP]; Jahu Integrated Faculties (FIJ); Universidade Estadual Paulista (UNESP)Currently, there is an increase in the demand for sports and physical exercises, which are the most effective way to promote health, self-esteem and prevent bad habits. In this context, the practice of CrossFit® attracts new practitioners every day, given the expectation of the challenge linked to the practice due to high intensity training, in this sense still, many feel attracted to competitions in the modality. However, high training loads associated with demands can bring changes in the level of stress associated with abrupt changes in mood. Thus, the present study aims to identify and analyze levels of stress and recovery, and the mood of CrossFit® competitors and non-competitors. The BRUMS instrument was used to measure the mood level and the RESTQ 76 to determine the levels of stress and recovery. The results demonstrate that there was a difference between the pre-competitive and competitive periods for the group of competitors for the variables tension (48.27 ± 36.36%, p = 0.05) and post-competition (79.31 ± 20.45%, p = 0.002), intergroup difference during the competitive period for depression [1380 ± 180.64%, X²(2) = 3.961, p = 0.05]; changes in anger were observed during pre-competitive and competitive periods (247.93 ± 33.14%, p = 0.041) for the competitive group, and during the recovery period (43.38 ± 27.64%, p = 0.048), in addition, difference between the analysis groups [109.45 ± 34.83%, X² (2) = 3.116, p = 0.043], vigor showed a significant reduction for the competitor group, and, intergroup difference during the study [X² (2) = 4,685; p = 0.03], in addition, there was a difference between groups for confusion (406.97 ± 25.98%, f = 4.707, p= 0.041); in the stress analysis, the specific stress showed a difference for the competitor group (31.15 ± 15.78%, p = 0.021), in addition to demonstrating a difference in the analysis between groups (39.94 ± 25.54%, f = 7.321, p = 0.019), the global stress in turn, it showed a difference between the groups (43.77 ± 36%, f = 5.068, p = 0.44). The data therefore demonstrate that CrossFit practitioners submitted to periods of competition have changes in their mood profile and stress levels when compared to non-competitive individuals submitted to the same training routines. ItemArtigoCharacterizing the interindividual postexercise hypotension response for two order groups of concurrent training in patients with morbid obesity(2022-10-10) Álvarez, Cristian; Guede-Rojas, Francisco; Ramírez-Campillo, Rodrigo; Andrade, David C.; Vásquez-Gómez, Jaime; Rodríguez-Rodríguez, Fernando; Ciolac, Emmanuel Gomes [UNESP]; Caamaño-Navarrete, Felipe; Delgado-Floody, Pedro; Universidad Andres Bello; Universidad de Antofagasta; Universidad Católica del Maule; Pontificia Universidad Católica de Valparaiso; Universidade Estadual Paulista (UNESP); Universidad Autónoma de Chile; Universidad de La Frontera; University of GranadaBackground: Postexercise hypotension (PEH) is a common physiological phenomenon occurring immediately after endurance training (ET), resistance training (RT), and ET plus RT, also termed concurrent training (CT); however, there is little knowledge about the interindividual and magnitude response of PEH in morbidly obese patients. Aim: The aims of this study were (1) to investigate the effect of CT order (ET + RT vs. RT + ET) on the blood pressure responses; 2) characterize these responses in responders and nonresponders, and 3) identify potential baseline outcomes for predicting blood pressure decreases as responders. Methods: A quasi-experimental study developed in sedentary morbidly obese men and women (age 43.6 ± 11.3 years; body mass index [BMI] ≥40 kg/m2) was assigned to a CT group of ET plus RT (ET + RT; n = 19; BMI 47.8 ± 16.7) or RT plus ET order group (RT + ET; n = 17; BMI 43.0 ± 8.0). Subjects of both groups received eight exercise sessions over four weeks. Primary outcomes include systolic (SBP), diastolic (DBP), mean arterial pressure [MAP], heart rate at rest [HR], and pulse pressure [PP] measurements before and after 10 min post-exercise. Secondary outcomes were other anthropometric, body composition, metabolic, and physical fitness parameters. Using the delta ∆SBP reduction, quartile categorization (Q) in “high” (Rs: quartile 4), “moderate” (MRs: quartile 3), “low” (LRs: quartile 2), and “nonresponders” (NRs: quartile 1) was reported. Results: Significant pre–post changes were observed in ET + RT in session 2 for SBP (131.6 vs. 123.4 mmHg, p = 0.050) and session 4 (131.1 vs. 125.2 mmHg, p = 0.0002), while the RT + ET group showed significant reductions in session 4 (134.2 vs. 125.3 mmHg, p < 0.001). No significant differences were detected in the sum of the eight sessions for SBP (∑∆SBP) between ET + RT vs. RT + ET (−5.7 vs. −4.3 mmHg, p = 0.552). Interindividual analyses revealed significant differences among frequencies comparing Q1 “NRs” (n = 8; 22.2%), Q2 “LRs” (n = 8; 22.2%), Q3 “MRs” (n = 9; 25.0%), and Q4 “HRs” (n = 11; 30.5%), p < 0.0001. Quartile comparisons showed significant differences in SBP changes (p = 0.035). Linear regression analyses revealed significant association between ∑∆SBP with body fat % (β –3.826, R2 0.211 [21.1%], p = 0.031), skeletal muscle mass [β –2.150, R2 0.125 (12.5%), p = 0.023], fasting glucose [β 1.273, R2 0.078 (7.8%), p = 0.003], triglycerides [β 0.210, R2 0.014 (1.4%), p = 0.008], and the 6-min walking test [β 0.183, R2 0.038 (3.8%), p = 0.044]. Conclusion: The CT order of ET + RT and RT + ET promote a similar ‘magnitude’ in the postexercise hypotensive effects during the eight sessions of both CT orders in 4 weeks of training duration, revealing “nonresponders” and ‘high’ responders that can be predicted from body composition, metabolic, and physical fitness outcomes. ItemArtigoCardiovascular, Respiratory, and Functional Effects of Home-Based Exercise Training after COVID-19 Hospitalization(2022-11-01) Teixeira Do Amaral, Vanessa [UNESP]; Viana, Ariane Aparecida [UNESP]; Heubel, Alessandro Domingues; Linares, Stephanie Nogueira; Martinelli, Bruno; Witzler, Pedro Henrique Camprigher [UNESP]; Orikassa De Oliveira, Gustavo Yudi [UNESP]; Zanini, Gabriel De Souza [UNESP]; Borghi Silva, Audrey; Mendes, Renata Goncąlves; Ciolac, Emmanuel Gomes [UNESP]; Universidade Estadual Paulista (UNESP); Universidade Federal de São Carlos (UFSCar); Sacred Heart University Center (UNISAGRADO)Introduction The present randomized, single-center, and single-blinded clinical trial tested the hypothesis that tele-supervised home-based exercise training (exercise) is an effective strategy for improving cardiovascular, respiratory, and functional capacity parameters in individuals who were hospitalized due to coronavirus disease 2019 (COVID-19). Methods Thirty-two individuals (52 ± 10 yr; 17 were female) randomly assigned to exercise (n = 12) or control groups (n = 20) had their anthropometric (weight, body mass index), hemodynamic (brachial and central blood pressure), vascular (arterial stiffness), ventilatory (pulmonary function and respiratory muscle strength), and functional parameters (handgrip strength, five-time sit to stand, timed up and go test, and 6-min walking test) assessed at baseline (30-45 d of hospital discharged) and after 12 wk of follow-up. Results Both groups similarly increased (P < 0.001) forced vital capacity (absolute and percent of predicted), forced expiratory volume in the first second (absolute and percent of predicted), and handgrip strength during follow-up. However, only the exercise group reduced carotid-femoral pulse wave velocity (-2.0 ± 0.6 m·s-1, P = 0.048) and increased (P < 0.05) resting oxygen saturation (1.9% ± 0.6%), mean inspiratory pressure (24.7 ± 7.1 cm H2O), mean expiratory pressure (20.3 ± 5.8 cm H2O), and percent of predicted mean expiratory pressure (14% ± 22%) during follow-up. No significant changes were found in any other variable during follow-up. Conclusions Present findings suggest that tele-supervised home-based exercise training can be a potential adjunct therapeutic to rehabilitate individuals who were hospitalized due to COVID-19. ItemArtigoPrescribing and Self-Regulating Heated Water-Based Exercise by Rating of Perceived Exertion in Older Individuals With Hypertension(2022-10-01) Marçal, Isabela Roque [UNESP]; Fernandes, Bianca [UNESP]; do Amaral, Vanessa Teixeira [UNESP]; Pelaquim, Renato Lopes; Ciolac, Emmanuel Gomes [UNESP]; Universidade Estadual Paulista (UNESP); Universidade de São Paulo (USP)We aimed to analyze the usefulness of the 6-20 rating of perceived exertion (RPE) scale for prescribing and self-regulating high-intensity interval (HIIE) and moderate-intensity continuous (MICE) aerobic exercise performed in a heated swimming pool (32 °C). Fifteen older individuals (65 ± 3 years) treated for hypertension underwent a symptom-limited maximal graded exercise test to determine their heart rate at anaerobic threshold, and respiratory compensation point. On different days, participants were randomized to HIIE (walking/jogging between 11 and 17 of RPE; 25 min) and MICE (walking at 11-13 of RPE; 30 min). Heart rate during the low-intensity intervals of HIIE and MICE remained below the graded exercise test's heart rate at anaerobic threshold (−7 ± 18 bpm/−16 ± 15 bpm) and respiratory compensation point (−18 ± 18 bpm/−30 ± 16 bpm), respectively, and maintained in the aerobic training zone during the high-intensity intervals of HIIE (+8 ± 18 bpm/−4 ± 19 bpm). The RPE scale at 15-17 is a useful tool for prescribing and self-regulating heated water-based HIIE and may have important implications for water-based exercise in older individuals with hypertension. ItemResenhaAn overview of the complex interaction between obesity and target organ dysfunction: focus on redox-inflammatory state(2023-06-01) Siqueira, Juliana Silva [UNESP]; Palacio, Thiago Luiz Novaga [UNESP]; Vieira, Taynara Aparecida [UNESP]; Nakandakare-Maia, Erika Tiemi [UNESP]; Grandini, Núbia Alves [UNESP]; Ferron, Artur Junio Togneri; Francisqueti-Ferron, Fabiane Valentini; Correa, Camila Renata [UNESP]; Universidade Estadual Paulista (UNESP); Integrated Colleges of Bauru (FIB)The high consumption of simple carbohydrates and saturated fat is associated with a positive energy balance and fat accumulation. This scenario has been contributing to increase obesity rates in the world population in the last decades. Adipose tissue dysfunction has been associated with early stages of obesity-associated comorbidities due to several direct and indirect mechanisms, such as unregulated adipokine secretion, macrophage infiltration, inflammation, reduced antioxidant defense, and oxidative stress. These conditions can result in several metabolic disorders, among them, hypertension, dyslipidemia, insulin resistance besides with tissue and organ dysfunction. Thus, the better understanding of the processes involved in the pathophysiology of obesity and adipose tissue dysfunction can help in preventive and treatment studies in order to reduce obesity consequences. ItemArtigoBergamot (Citrus bergamia) leaf extract improves metabolic, antioxidant and anti-inflammatory activity in skeletal muscles in a metabolic syndrome experimental model(2023-01-01) Palacio, Thiago Luiz Novaga [UNESP]; Siqueira, Juliana Silva [UNESP]; de Paula, Bruno Henrique [UNESP]; Rego, Rebeca Mayara Padilha [UNESP]; Vieira, Taynara Aparecida [UNESP]; Baron, Giovanna; Altomare, Alessandra; Ferron, Artur Junio Togneri [UNESP]; Aldini, Giancarlo; Kano, Hugo Tadashi [UNESP]; Correa, Camila Renata [UNESP]; Universidade Estadual Paulista (UNESP); University of Milan; Integrated Colleges of Bauru (FIB)Metabolic Syndrome (MetS), inflammation and oxidative stress contribute to impairment of skeletal muscle function. Bergamot (Citrus bergamia) leaf extract (BLE) has shown protective effects against comorbidities associated with MetS through its anti-inflammatory and antioxidant effects. The aim of this work was to elucidate the antioxidant and anti-inflammatory activity of BLE in skeletal muscles in an experimental model of MetS. Once metabolic syndrome was diagnosed, animals were divided into groups receiving different treatments for 10 weeks, including control diet (n = 10), control + BLE (n = 10), High Sugar-fat diet (HSF) (n = 10), HSF + BLE (n = 10). Evaluation included nutritional, metabolic and hormonal analyses, along with measurements of inflammatory status and oxidative stress in soleus and extensor digitorum longus (EDL) muscles. BLE showed positive metabolic effects, with a reduction of plasma triglycerides and insulin resistance and an increase in high-density lipoprotein cholesterol, and protective activity against oxidative stress and inflammation in Soleus and EDL muscles in animals with MetS. ItemArtigoMetabolic effect of low fluoride levels in the islets of NOD mice: integrative morphological, immunohistochemical, and proteomic analyses(2023-01-01) Trevizol, Juliana Sanches; Dionizio, Aline; Delgado, Aislan Quintiliano [UNESP]; Ventura, Talita Mendes Oliveira; Ribeiro, Caroline Fernanda da Silva; Ribeiro, Laura; Buzalaf, Nathalia Rabelo; Cestari, Tânia Mary; Magalhães, Ana Carolina; Suzuki, Maiko; Bosqueiro, José Roberto [UNESP]; Buzalaf, Marília Afonso Rabelo; Universidade de São Paulo (USP); Universidade Estadual Paulista (UNESP); Dental College of GeorgiaOBJECTIVES: Fluoride (F) has been widely used to control dental caries, and studies suggest beneficial effects against diabetes when a low dose of F is added to the drinking water (10 mgF/L). This study evaluated metabolic changes in pancreatic islets of NOD mice exposed to low doses of F and the main pathways altered by the treatment. METHODOLOGY: In total, 42 female NOD mice were randomly divided into two groups, considering the concentration of F administered in the drinking water for 14 weeks: 0 or 10 mgF/L. After the experimental period, the pancreas was collected for morphological and immunohistochemical analysis, and the islets for proteomic analysis. RESULTS: In the morphological and immunohistochemical analysis, no significant differences were found in the percentage of cells labelled for insulin, glucagon, and acetylated histone H3, although the treated group had higher percentages than the control group. Moreover, no significant differences were found for the mean percentages of pancreatic areas occupied by islets and for the pancreatic inflammatory infiltrate between the control and treated groups. Proteomic analysis showed large increases in histones H3 and, to a lesser extent, in histone acetyltransferases, concomitant with a decrease in enzymes involved in the formation of acetyl-CoA, besides many changes in proteins involved in several metabolic pathways, especially energy metabolism. The conjunction analysis of these data showed an attempt by the organism to maintain protein synthesis in the islets, even with the dramatic changes in energy metabolism. CONCLUSION: Our data suggests epigenetic alterations in the islets of NOD mice exposed to F levels comparable to those found in public supply water consumed by humans. ItemArtigoMechanical energy on anaerobic capacity during a supramaximal treadmill running in men: Is there influence between runners and active individuals?(Wiley-Blackwell, 2023-03-01) Zagatto, Alessandro Moura [UNESP]; Gonzalez, Joel Abraham Martinez [UNESP]; Poli, Rodrigo Araujo Bonetti de [UNESP]; Barbieri, Fabio Augusto [UNESP]; Bloedow, Leonardo de los Santos; Peyre-Tartaruga, Leonardo; Universidade Estadual Paulista (UNESP); Univ Autonoma Estado Mexico; Univ Fed Rio Grande Do SulThis study verified whether mechanical variables influence the anaerobic capacity outcome on treadmill running and whether these likely influences were dependent of running experience. Seventeen physical active and 18 amateur runners, males, performed a graded exercise test and constant load exhaustive running efforts at 115% of intensity associated to maximal oxygen consumption. During the constant load were determined the metabolic responses (i.e., gas exchange and blood lactate) to estimate the energetic contribution and anaerobic capacity as well as kinematic responses. The runners showed higher anaerobic capacity (16.6%; p = 0.005), but lesser time to exercise failure (-18.8%; p = 0.03) than active subjects. In addition, the stride length (21.4%; p = 0.00001), contact phase duration (-11.3%; p = 0.005), and vertical work (-29.9%; p = 0.015). For actives, the anaerobic capacity did not correlate significantly with any physiologic, kinematic, and mechanical variables and no regression model was fitted using the stepwise multiple regression, while to runners the anaerobic capacity was significantly correlated with phosphagen energetic contribution (r = 0.47; p = 0.047), external power (r = -0.51; p = 0.031), total work (r = -0.54; p = 0.020), external work (r = -0.62; p = 0.006), vertical work (r = -0.63; p = 0.008), and horizontal work (r = -0.61; p = 0.008), and the vertical work and phosphagen energetic contribution presented a coefficient of determination of 62% (p = 0.001). Based on findings, it is possible to assume that for active subjects, the mechanical variables have no influence over the anaerobic capacity, however, for experienced runners, the vertical work and phosphagen energetic contribution have relevant effect over anaerobic capacity output. ItemArtigoIs BDNF related to spatial-temporal gait parameters in people with multiple sclerosis? An observational study(2022-10-01) Santinelli, Felipe Balistieri [UNESP]; Sebastião, Emerson; Simieli, Lucas [UNESP]; Antunes, Barbara Moura [UNESP]; Vieira, Luiz Henrique Palucci [UNESP]; Kalron, Alon; Barbieri, Fabio Augusto [UNESP]; Hasselt University; Universidade Estadual Paulista (UNESP); Northern Illinois University; Universidad Autónoma de Baja California; Sagol School of NeuroscienceBackground: It has been suggested that the protein Brain-derived Neurotrophic Factor (BDNF) plays a neuroprotective role in people with multiple sclerosis (pwMS). Also, BDNF seems to play a role in cognition performance. In the same line, gait in pwMS requires a higher cognitive resource, mainly during complex walking. Thus, maybe BDNF could be related to gait in pwMS. Objective: To investigate the relationship between BDNF and gait spatial-temporal parameters during unobstructed and obstructed conditions and the Timed Up and Go (TUG) in pwMS and healthy controls (HC). Methods: The study included 20 pwMS (11F/9M, 33.1±7.5 years, Expanded Disability Status Scale- EDSS 2.2±1.2) and 18 HC (13F/5M, 35.5±5.9 years). Both groups performed 20 gait attempts in two conditions: unobstructed walking (10 trials) and avoiding an obstacle. The obstacle was 15 cm in height and made of foam material. The BDNF serum concentration was collected with participants in fasting and completed before the clinical, gait, and mobility assessments. Clinical variables included the Symbol Digit Modality Test (SDMT), the Fatigue Severity Scale (FSS), and the International Physical Activity Questionnaire (IPAQ- short version). Associations between BDNF and spatial-temporal gait parameters, clinical variables, and TUG were determined by Pearson/Spearman correlations with Bonferroni's correction being applied (p<0.0013). Gait was compared by a two-way, repeated-measures ANOVA (group and condition) to characterize our cohort. Results: Reduced BDNF was observed for pwMS (41.66±4.45 ng/ml) in comparison with HC (61.67±7.07, p<0.001). However, although some correlations presented a moderate correlation between BDNF with gait variables, the correlations didn't reach a significant p-value after Bonferroni's correction. Lastly, pwMS presented shorter step length and slower step velocity for both gait conditions, with more evidence for obstacle conditions. Only pwMS changed gait behavior from unobstructed walking to obstacle avoidance conditions (i.e., reduced step length and velocity and increased step duration). Conclusion: BDNF is not related to either clinical (i.e., EDSS, SDMT, FSS, or IPAQ) or gait parameters in pwMS and HC, even in a condition involving higher cognitive demand. These results may suggest that BDNF does not play a role in these parameters' performance. ItemArtigoCumulative additional information does not improve the neuromuscular control during postural responses to perturbations in postural instability/gait disorders subtype of Parkinson's disease(2022-09-01) Beretta, Victor Spiandor [UNESP]; Santos, Paulo Cezar Rocha [UNESP]; Orcioli-Silva, Diego [UNESP]; Jaimes, Diego Alejandro Rojas [UNESP]; Pereira, Marcelo Pinto [UNESP]; Barbieri, Fabio Augusto [UNESP]; Gobbi, Lilian Teresa Bucken [UNESP]; Universidade Estadual Paulista (UNESP); Weizmann Institute of Science; Universidade Estadual de Campinas (UNICAMP); Graduate Program in Physical Education and SportsBackground: Postural response impairments in postural instability and gait disorders (PIGD) subtype patients may be attributed to Parkinson's disease (PD)-deterioration in central-set (programing/modulating of central outputs during motor responses). Although additional information improves some PD motor impairments, an unanswered question is whether additional information can benefit postural response in PIGD subtype. Objective: To analyze the effect of cumulative additional information on postural responses after perturbation in PIGD and neurologically healthy older adults (CG). Methods: Perturbations were applied in 16 PIGD and 19 CG by the support-base translation. Participants performed 3 blocks of 5 trials without additional information (B1–B3, Day 1) and 5 trials of each cumulative additional information (C1–C4, Day 2): information about perturbation (C1), visual (C2), verbal (C3), and somatosensory information (C4). Electromyography and center of pressure (CoP) parameters were analyzed by ANOVAs with Group (PIGD × CG) and Block (B1 × B2 × B3) and with Group (PIGD × CG) and Condition (B3 × C1 × C2 × C3 × C4). Results: PIGD decreased the range of CoP in B3 while CG decreased both range of CoP and the integral of antagonist's muscle activity (iEMG) in B2. Also, PIGD decreased the recovery time in C4 while CG increased the iEMG of agonist's muscle in C2 and antagonist's muscle in all conditions except C2. Conclusion: Additional information provided before postural control assessment influences the postural response in PIGD and CG differently. PIGD demonstrated inflexibility of central-set in modulating the neuromuscular control regardless of additional information. CG presents a flexible system evidenced by the increase of agonist muscle iEMG when provided visual information. ItemArtigoEffects of Resonance Tube Voice Therapy on Parkinson's Disease: Clinical Trial(2022-01-01) dos Santos, Ana Paula; Troche, Michelle Shevon; Berretin-Felix, Giédre; Barbieri, Fabio Augusto [UNESP]; Brasolotto, Alcione Ghedini; Silverio, Kelly Cristina Alves; Universidade de São Paulo (USP); Columbia University; Universidade Estadual Paulista (UNESP)Purpose: To verify the effect of resonance tube voice therapy on the vocal aspects of patients with Parkinson's Disease (PD). Method: Intra-subject comparative controlled clinical trial with a single group assignment. Fourteen individuals with PD (10 men, mean age 66.1 years; four women, mean age 73.75 years) received eight 45-minute sessions of voice therapy, twice a week for 4 weeks. The therapy consisted of semi-occluded vocal tract exercises – phonation method in a resonance tube (glass, 27 cm x 9 mm) in water. Tube depth in water ranged from 2 cm to 9 cm, as the difficulty in carrying out the exercises increased (usual pitch, high pitch, low pitch, ascending/descending glissandos), followed by sentence production. The assessments were made three times: at baseline (Time0), after 30 days without intervention (Time1), and 1 day after eight intervention sessions (Time2). The following aspects were assessed: vocal intensity; acoustic parameters (Smoothed Cepstral Peak Prominence – CPPs, alpha ratio, and L1-L0 difference); auditory-perceptual analysis of the overall degree of vocal quality deviation; voice symptoms (Voice Symptom Scale protocol - VoiSS) and voice-related quality of life (Voice-Related Quality of Life Protocol – V-RQOL). The results were compared at the three times of assessment Time0/Time1/Time2 using one-way repeated measures ANOVA test and Tukey test (5% significance). Results: intervention significantly increased: vocal intensity, L1-L0 value of vowel /a/ and counting, CPP value in counting, and decreased: the overall degree of vocal quality deviation in 78% of participants, the total score of VoiSS protocol, the limitation, and emotional subscales. In addition, the intervention increased the score of all the domains of V-RQOL protocol - physical, socio-emotional, and total. Conclusion: Resonance tube phonation in voice therapy was effective in increasing vocal intensity and long-term acoustic parameters, the improved overall degree of vocal quality, reducing voice symptoms, and increasing voice-related quality of life in individuals with PD. ItemArtigoPerception of COVID-19 Pandemic by Brazilian People With Parkinson’s Disease and Multiple Sclerosis(2022-05-19) Simieli, Lucas [UNESP]; Santinelli, Felipe B. [UNESP]; Costa, Elisa C. [UNESP]; Kuroda, Marina H. [UNESP]; Oliveira, Lorena R. [UNESP]; Penedo, Tiago [UNESP]; Pilon, Julia [UNESP]; Silveira, Aline P. B. [UNESP]; Assis, Iramaia S. A. [UNESP]; Tardelli, Erica; Okamoto, Erika; Barbieri, Fabio A. [UNESP]; Universidade Estadual Paulista (UNESP); Hasselt University; Associação Brazil Parkinson (ABP)COVID-19 in Brazil is threatening, and it has forced the government to adopt partial lockdown as a strategy to stop the spread of the virus in the first wave of pandemic (March 2020). These preventive measures during the COVID-19 pandemic may affect the motor and non-motor symptoms in people with Parkinson’s disease (PD) and Multiple sclerosis (MS). Thus, the purpose of this study was to investigate the perception during the first wave of COVID-19 pandemic lockdown on motor and non-motor symptoms, and also measure physical activity level, quality of life, and sleep quality in Brazilian people with PD and MS. One hundred and fifty-three participants (PD-97 and MS-56) answered an online survey to identify the perception of motor and non-motor symptoms, and characterize the physical activity level, and quality of life and sleep in these neurological Brazillian population. During the beginning of pandemic lockdown in Brazil, our results indicated that 69% of people with PD and 55% of people with MS reported worse on motor aspects and lower amount of physical activity performed. Also, 75.2% (PD) and 92.9% (MS) of our cohort were considered inactive or sedentary. Based on the perception and behavior of the population studied, people with PD and MS should be encouraged to perform more physical activity in order to reduce the effects of isolation in motor and non-motor aspects of the diseases. Teleinterventions, such as home-based exercise, should be included in the new routine of people with PD and MS to reduce the impacts of lockdown and to maintain quality of life at a good level. ItemResenhaThe Effects of Overweight and Obesity on Obstacle Crossing During Walking: Protocol for a Systematic Review(2022-05-01) Chardon, Matthias [UNESP]; Barbieri, Fabio Augusto [UNESP]; Penedo, Tiago [UNESP]; Santos, Paulo Cezar Rocha; Vuillerme, Nicolas; Université Grenoble Alpes; Universidade Estadual Paulista (UNESP); Weizmann Institute of Science; Institut Universitaire de FranceBackground: Overweight and obesity are significant global health concerns that involve deficits in gait and balance that affect daily activities. Although much is reported about the effect of overweight and obesity on gait during unobstructed walking, not much is known about how overweight and obesity could impact gait under more challenging conditions, such as environments with obstacles. Objective: The aim of this study is to systematically review and synthesize the available data regarding the effects of overweight and obesity on obstacle crossing during walking. Methods: This review will follow the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-analyses) guidelines. PubMed, Web of Science, Scopus, and SPORTDiscus will be systematically searched with no limitations on publication date. Only full-text English-language articles published in a peer-reviewed journal will be included. Included articles must have compared obstacle crossing during walking in individuals with overweight or obesity to individuals of normal body weight. A total of 2 independent reviewers will select the articles and extract the following 4 sets of data: (1) study characteristics, (2) sample description, (3) obstacle crossing task protocol, and (4) main results obtained. If a considerable number of homogeneous papers are included, a meta-analysis will be conducted. A preliminary search was conducted in November 2021. Results: The results will include the article selection flowchart as well as tables and figures synthesizing the extracted data on the effects of overweight and obesity on obstacle crossing during walking. The preliminary search identified 73 original records, of which 5 articles met the inclusion criteria. Conclusions: This review will present researchers and clinicians with an overview of published studies that have compared the performance of obstacle crossing for individuals with overweight and obesity to those of normal body weight. Gaining insight into the control strategies adopted by individuals with overweight and obesity is critical for safe and successful obstacle crossing in this population. We therefore believe that our findings could be useful for identifying people at risk of falls and developing and implementing fall prevention programs for individuals with overweight and obesity. ItemArtigoCan Previous Levels of Physical Activity Affect Risk Factors for Cardiorespiratory Diseases and Functional Capacity after COVID-19 Hospitalization? A Prospective Cohort Study(2022-01-01) Viana, Ariane Aparecida [UNESP]; Heubel, Alessandro Domingues; Do Amaral, Vanessa Teixeira [UNESP]; Linares, Stephanie Nogueira; De Oliveira, Gustavo Yudi Orikassa [UNESP]; Martinelli, Bruno; Borghi Silva, Audrey; Mendes, Renata Gonçalves; Ciolac, Emmanuel Gomes [UNESP]; Universidade Estadual Paulista (UNESP); Universidade Federal de São Carlos (UFSCar); Centro Universitário Do Sagrado Coração (UNISAGRADO)Purpose. To evaluate the influence of previous levels of physical activity on hemodynamic, vascular, ventilatory, and functional outcomes after coronavirus disease 2019 (COVID-19) hospitalization. Methods. Sixty-three individuals with COVID-19 had their clinical status and previous levels (12 month) of physical activity (Baecke Questionnaire of Habitual Physical Activity) assessed at hospital admission. Individuals were then allocated to lower levels of physical activity (ACTLOWER; N=22), intermediate levels of physical activity (ACTINTERMEDIATE; N=22), or higher levels of physical activity (ACTHIGHER; N=19) groups, according to tertiles of physical activity. Resting hemodynamic (heart rate and brachial/central blood pressures) and vascular (carotid-femoral pulse wave velocity, augmentation index, and brachial artery flow-mediated dilation) variables, pulmonary function (spirometry), respiratory muscle strength (maximal respiratory pressures), and functional capacity (handgrip strength, five-time sit-to-stand, timed-up and go, and six-minute walking tests) were measured at 30 to 45 days after hospital discharge. Results. ACTLOWER showed lower levels (P<0.05) of forced vital capacity, forced expiratory volume in the first second, maximal voluntary ventilation, and maximal expiratory pressure than ACTHIGHER. ACTLOWER also had lower (P=0.023) walking distance (21%,) and lower percentage of predicted walking distance (20%) at six-minute walking test during follow-up than ACTINTERMEDIATE. However, hemodynamic and vascular variables, handgrip strength, five-time sit-to-stand, and timed-up and go were not different among groups. Conclusion. ACTLOWER showed impaired ventilatory parameters and walking performance when compared with ACTHIGHER and ACTINTERMEDIATE, respectively. These results suggest that previous levels of physical activity may impact ventilatory and exercise capacity outcomes 30 to 45 days after COVID-19 hospitalization discharge.