Departamento: Departamento de Ciências Biológicas
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Faculdade de Ciências Farmacêuticas
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Campus: Araraquara
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PublicaçãoArtigo Triatominae survey (Hemiptera: Reduviidae: Triatominae) in the south-central region of the state of Bahia, Brazil between 2008 and 2013(Amer Soc Trop Med &hygiene, 2015-05-01) Mendonca, Vagner José ; Oliveira, Jader de
; Rimoldi, Aline ; Ferreira Filho, Julio César Rente ; Araujo, Renato Freitas de ; Rosa, João Aristeu da ; Universidade de Brasília (UnB) ; Universidade Estadual Paulista (Unesp) ; Universidade Estadual de Campinas (UNICAMP) ; Universidade de São Paulo (USP) ; Secretaria Estadual de Saúde da Bahia (SESAB) Triatomine surveillance in rural areas, artificial ecotypes, and natural ecotopes of the cities of Caturama, Ibipitanga, Macaubas, and Seabra in the south-central region of the Brazilian state of Bahia was carried out between 2008 and 2013. Natural infection by Trypanosoma cruzi was evaluated in the specimens collected to monitor vectors of Chagas disease. A total of 1,357 specimens were collected, and four species were identified: Triatoma sordida (83%), Triatoma lend (16.4%), Triatoma pseudomaculata (0.5%), and Panstrongylus geniculatus (0.1%). Triatoma sordida was found in four cities, only 0.7% in intradomiciliary environments. Triatoma lend was found only in Macaubas; 8.5% were found in intradomiciliary environments, 88.3% in peridomiciliary environments, and 3.1% in sylvatic environments. Natural infection by T. cruzi was 0.5% for T. sordida and 3.1% T. lenti. All of these cases were found in peridomiciliary environments of Macaubas. As the results show, triatomines were found in intradomiciliary environments in three cities that were surveyed in the south-central region of the state of Bahia. Thus, an epidemiologic survey should be performed to avoid the risk of transmission to the population.PublicaçãoArtigo Mutagenic activity promoted by amentoflavone and methanolic extract of Byrsonima crassa Niedenzu(Elsevier B.V., 2006-08-01) Primila Cardoso, Cassia Regina ; Mara de Syllos Colus, Ilce ; Bernardi, Caroline Cristiane ; Sannomiya, Miriam ; Vilegas, Wagner; Varanda, Eliana Aparecida ; Universidade Estadual Paulista (Unesp) Byrsonima crassa is a plant pertaining to the Brazilian central savannah-like belt of vegetation and popularly used for the treatment of gastric dysfunctions and diarrhoea. The methanol extract contains catechin, tannins, terpenes and flavonoids; both mutagenic potential and antioxidant properties have been ascribed to flavonoids. The mutagenicity of some flavonoids is believed to be associated with the formation of reactive oxygen species and seems to depend on the number and position of hydroxyl groups. In the present study the mutagenic activity of the methanol, chloroform and 80% aqueous methanol extracts, as well as acetate and aqueous sub-fractions, of this medicinal plant were evaluated by Salmonella typhimurium assay, using strains 100, TA98, TA102 and TA97a, and in mouse reticulocytes. The results showed mutagenic activity of the methanolic extract in the TA98 strain without S9, but no mutagenicity to mouse cells in any of the extracts. The acetate fraction showed strong signs of mutagenicity without S9, suggesting that in this enriched fraction were concentrated the compounds that induced mutagenic activity. The aqueous fraction showed no mutagenic activity. The TLC and HSCCC analyses of the acetate fraction with some standard compounds permitted the isolation of the quercetin-3-O-beta-D-galactopyranoside, quercetin-3-O-alpha-L-arabinopyranoside, amentoflavone, methyl gallate and (+)-catechin, of which only the amentoflavone exhibited positive mutagenicity to TA98 (+S9, -S9). (c) 2006 Elsevier B.V.. All rights reserved.PublicaçãoArtigo 
Acesso Aberto Leukotrienes are not essential for the efficacy of a heterologous vaccine against Mycobacterium tuberculosis infection(Associação Brasileira de Divulgação Científica (ABRADIC), 2010-07-01) Franco, L. H. ; Paula, M. Oliveira e ; Wowk, P. F. ; Fonseca, D. M. da ; Sérgio, C. A. ; Fedatto, P. F. ; Gembre, A. F. ; Ramos, S. G. ; Silva, C. L. ; Medeiros, Alexandra Ivo de; Faccioli, L. H. ; Bonato, V. L. D. ; Universidade de São Paulo (USP) ; Universidade Estadual Paulista (Unesp) Leukotrienes are reported to be potent proinflammatory mediators that play a role in the development of several inflammatory diseases such as asthma, rheumatoid arthritis and periodontal disease. Leukotrienes have also been associated with protection against infectious diseases. However, the role of leukotrienes in Mycobacterium tuberculosis infection is not understood. To answer this question, we studied the role of leukotrienes in the protective immune response conferred by prime-boost heterologous immunization against tuberculosis. We immunized BALB/c mice (4-11/group) with subcutaneous BCG vaccine (1 x 10(5) M. bovis BCG) (prime) followed by intramuscular DNA-HSP65 vaccine (100 µg) (boost). During the 30 days following the challenge, the animals were treated by gavage daily with MK-886 (5 mg·kg-1·day-1) to inhibit leukotriene synthesis. We showed that MK-886-treated mice were more susceptible to M. tuberculosis infection by counting the number of M. tuberculosis colony-forming units in lungs. The histopathological analysis showed an impaired influx of leukocytes to the lungs of MK-886-treated mice after infection, confirming the involvement of leukotrienes in the protective immune response against experimental tuberculosis. However, prime-boost-immunized mice treated with MK-886 remained protected after challenge with M. tuberculosis, suggesting that leukotrienes are not required for the protective effect elicited by immunization. Protection against M. tuberculosis challenge achieved by prime-boost immunization in the absence of leukotrienes was accompanied by an increase in IL-17 production in the lungs of these animals, as measured by ELISA. Therefore, these data suggest that the production of IL-17 in MK-886-treated, immunized mice could contribute to the generation of a protective immune response after infection with M. tuberculosis.PublicaçãoArtigo Antimycobacterial activity of Indigofera suffruticosa with activation potential of the innate immune system(Taylor & Francis Inc, 2010-08-01) Carli, Camila B. de A.; Quilles, Marcela B. ; Maia, Daniele C. G. ; Lopes, Flavia C. M. ; Santos, Rubens ; Pavan, Fernando R. ; Fujimura Leite, Clarice Q. ; Calvo, Tamara Regina ; Vilegas, Wagner ; Carlos, Iracilda Z. ; Universidade Estadual Paulista (Unesp) Mycobacterium tuberculosis is responsible for over 8 million cases of tuberculosis (TB) annually. Natural products may play important roles in the chemotherapy of TB. The antimycobacterial activity and the innate immune response of methanol (METH) and dichloromethane (DCM) extracts of Indigofera suffruticosa Miller (Fabaceae) were evaluated. We observed that the minimum inhibitory concentrations (MICs) for METH and DCM extracts were 125 and 1000 mu g/mL, respectively. However, they were able to induce the innate immune response through the production of high levels of NO and TNF-alpha (p < 0.001) by peritoneal exudate cells (PECs). These results suggest that I. suffruticosa extracts may have an important immunological role in the control of TB once macrophage activity is induced by them.PublicaçãoArtigo INCIDENCE OF MYCOBACTERIUM-TUBERCULOSIS AND OTHER MYCOBACTERIA ON PULMONARY INFECTIONS IN ARARAQUARA - SP, 1993(Soc Brasileira Microbiologia, 1995-04-01) Leite, CQF ; Viana, BHJ ; Leite, SRA ; Juarez, E. ; Universidade Estadual Paulista (Unesp)The incidence of tuberculosis and other infections by mycobacteria was analyzed in 559 patients admitted to the Tisiology Section of the Special Health Care Unit of Araraquara (SESA). Mycobacteria were isolated from 78 individuals out of this total. Among these patients, 15 were also HIV positive. The occurrence of isolated species was: M. tuberculosis: 69 patients; M. avium-intracellulare: 5 patients; M. fortuitum: 2 patients; M. chelonae: 1 patient; and M. simiae 1 patient. The latter was for the first time isolated from humans in Brazil. In most cases, non tubercular mycobacteria (NTM) were found in the HIV positive patients.PublicaçãoArtigo Acesso Aberto Acesso, segurança e uso de medicamentos por usuários(2012) Andrade, Raul Vasconcelos; Farache Filho, Adalberto ; Galduróz, José Carlos ; Mastroianni, Patricia de Carvalho ; Universidade Estadual Paulista (Unesp) ; Universidade Federal de São Paulo (UNIFESP) The present study aims to identify families who have stock and are making use of medications, as well as assess the conditions of storage, security and use of these drugs. The study was conducted in a city of São Paulo, the interviews were conducted in households enrolled in one of ten units of the Estratégia de Saúde da Família (ESF) that the city provides and the sample was defined by means of stratified random sampling (134households, IC 95%). Data collection was conducted through interviews with a semistructured questionnaire during the first half of 2011. We interviewed 118 (88.0%) households, of which 112 (95.0%) had medications that were stored n insecure or inadequate places in 75.4% of households, non-prescription self-medication was a common practice in 46 (47 4%) households, and lack of identification and security of medications stored was observed in 60 (53.6%) households. Most households had stock of medicines, which were done improperly or unsecure, or have specialties with lack of identification and security, which can lead to poisoning or e ineffective therapy. The Pharmaceutical Assistance under SUS lacks social initiatives, with actions directed for medications users, which can be supplied by the presence of the pharmacist in the ESF, essential for the promotion of racional use of medicines, that, through the Pharmaceutical Care, can identify, correct and prevent possible problems related to drugs.PublicaçãoArtigo Acesso Aberto Using captive sentinels to collect wild triatomines in the region of Marília-SP, Brasil(2013) Martins, Luciamáre Perrinetti Alves ; Castanho, Roberto Esteves Pires ; Tonon, Felipe Frasão ; Unterleider, Carlos Eduardo ; Rosa, João Aristeu da; Universidade Estadual Paulista (Unesp) In order to collect wild triatomines in the municipality of Marilia, two captive sentinel sites were installed in a remnant riparian zone in the Atlantic Forest comprising 34 hectares located on the Nossa Senhora Aparecida farm. One enclosure was built on the margin and another inside the forest, about 200 meters from the edge, using rabbits as bait to attract wild triatomines. In addition to the caged rabbits, active searches for triatomines were performed in tree holes, bird nests and armadillo burrows by spraying Pirisa 1080-L1E1 aerosol dislodger. The sentinels were maintained for one year and four months. During this period six active searches were performed, but without success in attracting or colonizing wild triatomines. One factor that has probably intervened in the triatomine colonization is the periodic application of insecticides formulated with pyrethroid in farms neighboring the forest to fight Diaphorina citri in orange plantations.PublicaçãoArtigo Genetic data of 10 X-chromosomal loci in Vitória population (Espírito Santo State, Brazil)(2009-01-01) Martins, J. A.; Costa, J. C. ; Paneto, G. G. ; Gusmão, L. ; Sánchez-Diz, P. ; Carracedo, A. ; Cicarelli, Regina Maria Barretto ; Universidade Estadual Paulista (Unesp) ; University of Porto ; University of Santiago de Compostela Genetic population data for 10 X-STR (DXS8378, DXS9898, DXS7133, GATA31E08, GATA172D05, DXS7423, DXS6809, DXS7132, DXS9902 and DXS6789) were obtained from Vitória population (Espírito Santo State, Brazil). No deviations from the Hardy-Weinberg equilibrium and linkage disequilibrium were observed. The combined powers of discrimination in males and females were 0.9999995 and 0.99999999996, respectively. These high values show the potential of this system in human identification in Vitória population, Brazil. © 2009 Elsevier B.V. All rights reserved.PublicaçãoArtigo Acesso Aberto Soropositividade para doença de Chagas entre doadores de sangue em Araraquara, Estado de São Paulo, no período de 2004 a 2008(Sociedade Brasileira de Medicina Tropical - SBMT, 2011-02-01) Ferreira Filho, Julio César Rente; Costa, Paulo Inácio da ; Buainain, Arnaldo ; Rosa, João Aristeu da ; Universidade Estadual Paulista (Unesp) ; Laboratório Dr. Arnaldo Buainain INTRODUÇÃO: A transfusão sanguínea é uma fonte potencial importante para transmissão da doença de Chagas. MÉTODOS: Foi verificado, nos arquivos do Hemonúcleo Regional de Araraquara, o resultado dos exames para doença de Chagas entre janeiro de 2004 e dezembro de 2008. RESULTADOS: Foram diagnosticadas com sorologia positiva 0,04% das 49541 doações de sangue realizadas. A idade dos soropositivos situou-se entre 51 e 60 anos. CONCLUSÕES: O baixo índice de doadores soropositivos pode reduzir o risco de transmissão via transfusional da doença de Chagas. A alta ocorrência de resultados inconclusivos indicam que os métodos diagnósticos devem ser melhorados.PublicaçãoArtigo Acesso Aberto Impairment of neutrophil migration to remote inflammatory site during lung histoplasmosis(Hindawi Publishing Corporation, 2015-01-01) Medeiros, Alexandra Ivo de; Secatto, Adriana ; Belanger, Caroline ; Sorgi, Carlos Artério ; Borgeat, Pierre ; Marleau, Sylvie ; Faccioli, Lucia Helena ; Universidade Estadual Paulista (Unesp) ; Universidade de São Paulo (USP) ; Université de Montréal ; Centre Hospitalier Universitaire de Québec (CHUQ) Histoplasma capsulatum (Hc) induces a pulmonary disease in which leukotrienes promote activation and recruitment of effectors cells. It is also well-recognized that leukotriene B 4 (LTB 4) and platelet-activating factor (PAF) induce leukocyte recruitment to inflammatory sites. We investigated the impact of pulmonary Hc infection on PMN migration to a remote inflammatory site. Our results show that pulmonary Hc infection impairs LTB4- or PAF-stimulated PMN recruitment to air pouch. Yet, remote inflammation did not modify PMN numbers in the bronchoalveolar lavage fluid (BALF) of Hc-infected mice. Interestingly, the concomitant administration of PAF and LTB 4 receptor antagonists inhibited PMN recruitment to both BALF and the remote site, demonstrating cooperation between both mediators. Along that line, our results show that PAF-elicited PMN chemotaxis was abrogated in 5-lipoxygenase-deficient animals. These results suggest caution in the indiscriminate use of anti-inflammatory drugs during infectious diseases.