Caveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma
dc.contributor.author | Govoni, Verônica Mollica [UNESP] | |
dc.contributor.author | Pigoli, Claudio | |
dc.contributor.author | Brambilla, Eleonora | |
dc.contributor.author | Ruiz Sueiro, Felipe Augusto | |
dc.contributor.author | Torres Neto, Rafael | |
dc.contributor.author | Laufer-Amorim, Renee [UNESP] | |
dc.contributor.author | Gomes Quitzan, Juliany [UNESP] | |
dc.contributor.author | Grieco, Valeria | |
dc.contributor.author | Fonseca-Alves, Carlos Eduardo [UNESP] | |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | |
dc.contributor.institution | Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER) | |
dc.contributor.institution | Università degli Studi di Milano | |
dc.contributor.institution | VetPat Laboratory | |
dc.contributor.institution | VetMol Laboratory | |
dc.contributor.institution | Paulista University – UNIP | |
dc.date.accessioned | 2023-07-29T13:25:32Z | |
dc.date.available | 2023-07-29T13:25:32Z | |
dc.date.issued | 2022-10-10 | |
dc.description.abstract | The bladder urothelial carcinoma (UC) represents ~2% of malignant neoplasms in dogs and is a therapeutic challenge in veterinary medicine. Although it is considered the most common bladder cancer in dogs, few previous studies have investigated different markers that correlate with clinical and pathological parameters. Therefore, this study aimed to evaluate Caveolin-1, GATA-3, and Ki67 immunostaining in canine UC samples to evaluate their correlations with histopathological variables. Thirty tumor samples were obtained, and Caveolin-1, GATA-3, and Ki67 expression was assessed by immunohistochemistry and associated with pathological factors by univariate and multivariate analyses. Among the histopathological findings, lymphatic invasion was identified in 53.33% of the tumors, and the mean mitotic count (MC) was 31.82 ± 26.26. Caveolin-1 showed mild-to-high cytoplasmic expression in neoplastic cells, whereas GATA-3 showed mild-to-high nuclear expression. The Ki67 expression revealed a mean of 24.14 ± 16.88% positive cells. In the univariate analysis, no association was found between each marker and the pathological findings. On the other hand, in multivariate analysis, we identified a positive correlation between GATA-3 and MC and a negative correlation between Caveolin-1 and MC. Moreover, lymphatic invasion was positively correlated with histological type and grade, and negatively correlated with MC. In addition, the histological type was positively correlated with the histological grade. Overall, our results indicate that Caveolin-1 and GATA-3 expression could be promising markers for bladder UC aggressiveness. | en |
dc.description.affiliation | Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University – UNESP | |
dc.description.affiliation | Laboratorio di Istologia Sede Territoriale di Milano Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER) | |
dc.description.affiliation | Department of Veterinary Medicine and Animal Sciences Università degli Studi di Milano | |
dc.description.affiliation | VetPat Laboratory | |
dc.description.affiliation | VetMol Laboratory | |
dc.description.affiliation | Department of Veterinary Clinic School of Veterinary Medicine and Animal Science São Paulo State University – UNESP | |
dc.description.affiliation | Institute of Health Sciences Paulista University – UNIP | |
dc.description.affiliationUnesp | Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University – UNESP | |
dc.description.affiliationUnesp | Department of Veterinary Clinic School of Veterinary Medicine and Animal Science São Paulo State University – UNESP | |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
dc.description.sponsorshipId | CNPq: 302977/2021-0 | |
dc.description.sponsorshipId | CNPq: 422139/2018-1 | |
dc.identifier | http://dx.doi.org/10.3389/fvets.2022.986269 | |
dc.identifier.citation | Frontiers in Veterinary Science, v. 9. | |
dc.identifier.doi | 10.3389/fvets.2022.986269 | |
dc.identifier.issn | 2297-1769 | |
dc.identifier.scopus | 2-s2.0-85140325211 | |
dc.identifier.uri | http://hdl.handle.net/11449/247777 | |
dc.language.iso | eng | |
dc.relation.ispartof | Frontiers in Veterinary Science | |
dc.source | Scopus | |
dc.subject | biomarker | |
dc.subject | cancer | |
dc.subject | dog | |
dc.subject | immunohistochemistry | |
dc.subject | neoplasia | |
dc.title | Caveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma | en |
dc.type | Artigo | |
dspace.entity.type | Publication | |
unesp.campus | Universidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária e Zootecnia, Botucatu | pt |
unesp.department | Clínica Veterinária - FMVZ | pt |
unesp.department | Reprodução Animal e Radiologia Veterinária - FMVZ | pt |