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Caveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma

dc.contributor.authorGovoni, Verônica Mollica [UNESP]
dc.contributor.authorPigoli, Claudio
dc.contributor.authorBrambilla, Eleonora
dc.contributor.authorRuiz Sueiro, Felipe Augusto
dc.contributor.authorTorres Neto, Rafael
dc.contributor.authorLaufer-Amorim, Renee [UNESP]
dc.contributor.authorGomes Quitzan, Juliany [UNESP]
dc.contributor.authorGrieco, Valeria
dc.contributor.authorFonseca-Alves, Carlos Eduardo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionIstituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER)
dc.contributor.institutionUniversità degli Studi di Milano
dc.contributor.institutionVetPat Laboratory
dc.contributor.institutionVetMol Laboratory
dc.contributor.institutionPaulista University – UNIP
dc.date.accessioned2023-07-29T13:25:32Z
dc.date.available2023-07-29T13:25:32Z
dc.date.issued2022-10-10
dc.description.abstractThe bladder urothelial carcinoma (UC) represents ~2% of malignant neoplasms in dogs and is a therapeutic challenge in veterinary medicine. Although it is considered the most common bladder cancer in dogs, few previous studies have investigated different markers that correlate with clinical and pathological parameters. Therefore, this study aimed to evaluate Caveolin-1, GATA-3, and Ki67 immunostaining in canine UC samples to evaluate their correlations with histopathological variables. Thirty tumor samples were obtained, and Caveolin-1, GATA-3, and Ki67 expression was assessed by immunohistochemistry and associated with pathological factors by univariate and multivariate analyses. Among the histopathological findings, lymphatic invasion was identified in 53.33% of the tumors, and the mean mitotic count (MC) was 31.82 ± 26.26. Caveolin-1 showed mild-to-high cytoplasmic expression in neoplastic cells, whereas GATA-3 showed mild-to-high nuclear expression. The Ki67 expression revealed a mean of 24.14 ± 16.88% positive cells. In the univariate analysis, no association was found between each marker and the pathological findings. On the other hand, in multivariate analysis, we identified a positive correlation between GATA-3 and MC and a negative correlation between Caveolin-1 and MC. Moreover, lymphatic invasion was positively correlated with histological type and grade, and negatively correlated with MC. In addition, the histological type was positively correlated with the histological grade. Overall, our results indicate that Caveolin-1 and GATA-3 expression could be promising markers for bladder UC aggressiveness.en
dc.description.affiliationDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University – UNESP
dc.description.affiliationLaboratorio di Istologia Sede Territoriale di Milano Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER)
dc.description.affiliationDepartment of Veterinary Medicine and Animal Sciences Università degli Studi di Milano
dc.description.affiliationVetPat Laboratory
dc.description.affiliationVetMol Laboratory
dc.description.affiliationDepartment of Veterinary Clinic School of Veterinary Medicine and Animal Science São Paulo State University – UNESP
dc.description.affiliationInstitute of Health Sciences Paulista University – UNIP
dc.description.affiliationUnespDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University – UNESP
dc.description.affiliationUnespDepartment of Veterinary Clinic School of Veterinary Medicine and Animal Science São Paulo State University – UNESP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: 302977/2021-0
dc.description.sponsorshipIdCNPq: 422139/2018-1
dc.identifierhttp://dx.doi.org/10.3389/fvets.2022.986269
dc.identifier.citationFrontiers in Veterinary Science, v. 9.
dc.identifier.doi10.3389/fvets.2022.986269
dc.identifier.issn2297-1769
dc.identifier.scopus2-s2.0-85140325211
dc.identifier.urihttp://hdl.handle.net/11449/247777
dc.language.isoeng
dc.relation.ispartofFrontiers in Veterinary Science
dc.sourceScopus
dc.subjectbiomarker
dc.subjectcancer
dc.subjectdog
dc.subjectimmunohistochemistry
dc.subjectneoplasia
dc.titleCaveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinomaen
dc.typeArtigo
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt
unesp.departmentClínica Veterinária - FMVZpt
unesp.departmentReprodução Animal e Radiologia Veterinária - FMVZpt

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